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Conesa, A. (author), Punt, P.J. (author), Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Peroxidases are oxidoreductases that utilize hydrogen peroxide to catalyze oxidative reactions. A large number of peroxidases have been identified in fungal species and are being characterized at the molecular level. In this manuscript we review the current knowledge on the molecular aspects of this type of enzymes. We present an overview of the...article 2002
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Conesa, A. (author), Jeenes, D. (author), Archer, D.B. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author)Heme-containing peroxidases from white rot basidiomycetes, in contrast to most proteins of fungal origin, are poorly produced in industrial filamentous fungal strains. Factors limiting peroxidase production are believed to operate at the posttranslational level. In particular, insufficient availability of the prosthetic group which is required...article 2002
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Wolken, W.A.M. (author), van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Spores of Penicillium digitatum ATCC 201167 transform geraniol, nerol, citral, and geranic acid into methylheptenone. Spore extracts of P. digitatum convert geraniol and nerol NAD+-dependently into citral. Spore extract also converts citral NAD+-dependently into geranic acid. Furthermore, a novel enzymatic activity, citral lyase, which cofactor...article 2001
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Conesa, A. (author), Punt, P.J. (author), van Luijk, N. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The high capacity of the secretion machinery of filamentous fungi has been widely exploited for the production of homologous and heterologous proteins; however, our knowledge of the fungal secretion pathway is still at an early stage. Most of the knowledge comes from models developed in yeast and higher eukaryotes, which have served as reference...article 2001
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Punt, P.J. (author), Seiboth, B. (author), Weenink, X.O. (author), van Zeijl, C. (author), Lenders, M. (author), Konetschny, C. (author), Ram, A.F.J. (author), Montijn, R. (author), Kubicek, C.P. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. This isolation and the search for similar sequences in fungal genomic and EST databases showed that, in contrast to Saccharomyces cerevisiae, filamentous...article 2001
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Hoogschagen, M. (author), Zhu, Y. (author), van As, H. (author), Tramper, J. (author), Rinzema, A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The respiration kinetics of Aspergillus oryzae on different varieties of whole wheat kernels were studied. Six wheat varieties were pretreated in two different ways. Five of the six substrates fermented similarly and independently of the pretreatment method. However, pretreatment affected fermentation of one variety of soft wheat (Apollo). T21H...article 2001
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A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutationsTNO Preventie en Gezondheid (author), Raamsdonk, L.M. (author), Teusink, B. (author), Broadhurst, D. (author), Zhang, N. (author), Hayes, A. (author), Walsh, M.C. (author), Berden, J.A. (author), Brindle, K.M. (author), Kell, D.B. (author), Rowland, J.J. (author), Westerhoff, H.V. (author), van Dam, K. (author), Oliver, S.G. (author)A large proportion of the 6,000 genes present in the genome of Saccharomyces cerevisiae, and of those sequenced in other organisms, encode proteins of unknown function. Many of these genes are "silent," that is, they show no overt phenotype, in terms of growth rate or other fluxes, when they are deleted from the genome. We demonstrate how the...article 2001
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- Centraal Instituut voor Voedingsonderzoek TNO (author), Conesa, A. (author) doctoral thesis 2001
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Conesa, A. (author), Weelink, G. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author)The Caldariomyces fumago chloroperoxidase (CPO) is synthesised as a 372-aa precursor which undergoes two proteolytic processing events: removal of a 21-aa N-terminal signal peptide and of a 52-aa C-terminal propeptide. The Aspergillus niger expression system developed for CPO was used to get insight into the function of this C-terminal...article 2001
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Conesa, A. (author), van de Velde, F. (author), van Rantwijk, F. (author), Sheldon, R.A. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author)The Caldariomyces fumago chloroperoxidase was successfully expressed in Aspergillus niger. The recombinant enzyme was produced in the culture medium as an active protein and could be purified by a three-step purification procedure. The catalytic behavior of recombinant chloroperoxidase (rCPO) was studied and compared with that of native CPO. The...article 2001
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Conesa, A. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-deficient A. niger strain and...article 2000
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van den Brink, J.M. (author), Punt, P.J. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Cytochrome P450 enzyme systems are found throughout nature and are involved in many different, often complex, bioconversions. In the endoplasmic reticulum of the filamentous fungus Aspergillus niger a cytochrome P450 enzyme system is present that is capable of the para-hydroxylation of benzoate. The expression of the two genes encoding the...article 2000
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Müller, W.H. (author), Koster, A.J. (author), Humbel, B.M. (author), Ziese, U. (author), Verkleij, A.J. (author), van Aelst, A.C. (author), van der Krift, T.P. (author), Montijn, R.C. (author), Boekhout, T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hypal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the...article 2000
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Hempenius, R.A. (author), Lina, B.A.R. (author), Haggitt, R.C. (author)Arachidonic acid oil (ARA-oil) derived from the fungus Mortierella alpina for use in infant nutrition was tested in a subchronic (13-week) oral toxicity study in rats, preceded by an in utero exposure phase. The ARA-oil was administered as admixture to the rodent diet at dose levels of 3000 ppm, 15,000 ppm and 75,000 ppm. An additional high-dose...article 2000
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TNO Preventie en Gezondheid (author), Renirie, R. (author), Hemrika, W. (author), Piersma, S.R. (author), Wever, R. (author)The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectrum. Substrate-induced...article 2000
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Havermans, J.B.G.A. (author), Marres, P. (author), Defize, P. (author)A model for calculating the assessment or consultability of archives was developed. This model includes an archive sampling procedure, defining damage profiles, damage sub-profiles and the development of a classifier. Research showed that the whole model developed is reliable and can be carried out easily.article 1999
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Santerre Henriksen, A.L. (author), Even, S. (author), Müller, C. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Nielsen, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PglaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PglaA-driven GFP expression by the glucoamylase...article 1999
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Maras, M. (author), van Die, I. (author), Contreras, R. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to increase the production...article 1999
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van Gemeren, I.A. (author), Beijersbergen, A. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Several cutinase variants derived by molecular modelling and site- directed mutagenesis of a cutinase gene from Fusarium solani pisi are poorly secreted by Saccharomyces cerevisiae. The majority of these variants are successfully produced by the filamentous fungus Aspergillus awamori. However, the L51S and T179Y mutations caused reductions in...article 1998
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Schuren, F.H.J. (author), Wessels, J.G.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)GPD regulatory sequences were used to express a phleomycin resistance gene (Sh ble) in Schizophyllum commune, resulting in high numbers of phleomycin-resistant transformants. Attempts to express heterologous genes coding for hygromycin B phosphotransferase (hph), aminoglycoside phosphotransferase (apt), β-glucuronidase (uidA) and β-galactosidase...article 1998