Effect of air drying on bacterial viability: A multiparameter viability assessment

The effect of desiccation on the viability of microorganisms is a question of great interest for a variety of public health questions and industrial applications. Although viability is traditionally assessed by plate counts, cultivation-independent methods are increasingly applied with the aim to gain more insight into why cells might not form...

Changes in Water Content, Collagen Degradation, Collagen Content, and Concentration in Repeated Biopsies of the Cervix of Pregnant Cows

The objective of the present study was to assess if cervical ripeness could be quantified by measuring the percentage of denaturation of the collagen network of the stromal layer. Biopsy specimens from the caudal part of the cervix were obtained from nine pluriparous cows between Days 149 and 157 of gestation (second-trimester biopsy), at...

FLAIR intercomparisons on serum and red cell folate

Chemicals/CAS: 5 methyltetrahydrofolic acid, 134-35-0; folic acid, 59-30-3, 6484-89-5; 5-methyltetrahydrofolate, 134-35-0; Folic Acid, 59-30-3; Reagent Kits, Diagnostic; Tetrahydrofolates

Human diets cooked by microwave or conventionally: Comparative sub-chronic (13-wk) toxicity study in rats

To compare the possible effects of microwave and conventional cooking on a range of common dietary components, mixed human diets containing beef, potatoes and vegetables were fed to groups of 10 male and 10 female Wistar rats for 13 wk. The diet ingredients were cooked by either of the methods in a normal and an abused manner, the latter...

Determination of progesterone by liquid chromatography - mass spectrometry using a moving-belt interface and isotope dilution

Progesterone can be determined rapidly and accurately with the method presented, using isotope dilution LC-MS with a moving-belt interface. Deactivation is very useful in that it minimizes belt memory effects and optimizes belt desorption characteristics. Since the moving-belt properties were found to change during time with respect to the...

The aetiology of caecal enlargement in the rat

The effect of hydroxypropyl starches (degree of substitution (DS) 0.025-0.106), lactose, raw potato starch, polyethylene glycol 1000 or magnesium sulphate on caecal size and some constituents of the caecal contents was studied in male rats of ages varying from 4 wk to 3 mth in experiments lasting from 10 days to 3 mth. All the test compounds...

Influence of the radiosensitizers paranitroacetophenone and triacetoneamine N oxyl on the effects of ionizing radiation on DNA

Paranitroacetophenone (PNAP) and triacetoneamine N oxyl (TAN), which sensitize anoxic cells to ionizing radiation, do not increase the radiosensitivity of bacteriophage DNA irradiated in the dry state, showing that the 'direct action model' of sensitization does not hold in this experimental set up. PNAP and TAN added within 1.5 msec after...

The regulation of cell proliferation and differentiation in intestinal epithelium. II. A quantitative histochemical and autoradiographic study after low doses of X-irradiation

To investigate the biochemical mechanisms involved in the regulation of cell proliferation and differentiation the intestinal epithelium has been chosen because of the regular distribution of proliferative-, non-dividing maturing- and functional cells along the crypt and villus. The effect of low doses of irradiation (50-400 R) which selectively...