Repeated exposure to acrolein vapour: Subacute studies in hamsters, rats and rabbits

The subacute inhalation toxicity of acrolein was examined in 4 groups of 20 hamsters, 12 rats and 4 rabbits each, exposed repeatedly to acrolein vapour at concentrations of 0, 0.4, 1.4 and 4.9 ppm (6 hr/day, 5 days/week) for a 13-week period. The most important effects found at the highest level included mortality in rats, ocular and nasal...

Report of a workshop on bacterial in vitro mutagenicity test systems

Bacterial systems for the screening of chemical substances for mutagenic potential are receiving widespread attention. Now that many laboratories have introduced these test systems and a large number of substances has been tested, discrepancies in the results have become apparent. To discuss this problem, a workshop was organized at Neuherberg ...

Trophic support by neural explants of cultured muscle fibers

The influence of various neural explants on the morphology and the survival of chick muscle fibers was studied. A method was developed to evaluate the condition of the muscle fibers using the following four morphological parameters: cross striation, thickness, number of fibers and absence of vacuoles. Chick as well as mouse spinal cord explants...

Target recognition and synapse formation by ciliary-ganglion neurons in tissue culture

A less complicated source of neurons suitable for this type of studies is the parasympathetic ciliary ganglion. In the pigeon and in the chick this ganglion is known to contain only two classes of neurons, both of which are cholinoceptive and cholinergic and that innervate the muscle fibres of the choroid plexus of the eye, the sphincter iridis...

Multi-locus control of MLC reactions in rhesus monkeys

The genetic basis for MLC reactivity in rhesus monkeys was further investigated. Cells from an intra-RhLA recombinant monkey were MLC reactive with those of an SD-identical sib and with each of two sets of homozygous typing cells carrying either paternal haplotype. Also, cells from a pair of RhLA- identical sibs reacted in MLC with each other,...

Mutagenicity screening: General principles and minimal criteria. Report of a committee of the European Environmental Mutagen Society

A statement of general principles and minimal criteria for the screening of chemicals for potential mutagenicity in man that may be used as guidelines for regulatory agencies and industrial organisations. To make clear the potentialities and current limitations of short-term mutagenicity testing for the prediction of genetic and carcinogenic...

Successful oxime therapy one hour after soman intoxication in the rat

The bisquarternary mono-oxime HI-6, and to a lesser extent HS-6, caused functional recovery of neuromuscular transmission in vivo and in vitro when given 60 min after soman, i.e. when the soman-cholinesterase (AChE) complex is said to be fully 'aged'. Atropinised rats, with the tracheas intubated, received 4 x LD50 soman i.v. and were kept alive...

Breaks induced in the deoxyribonucleic acid of aerosolized Escherichia coli by ozonized cyclohexene

Chemicals/CAS: ozone, 10028-15-6; Aerosols; Cyclohexanes; DNA, Bacterial; Ozone, 10028-15-6

Purification of rat fibrinogen and its constituent chains

Rat fibrinogen was purified from rat plasma by using lysine-Sepharose chromatography, repeated precipitation with 25%-satd. (HN4)2SO4 and gel chromatography on Sepharose 6B. To minimize proteolytic activity, rats were injected intravenously with Trasylol before bleeding and the collected blood was treated with Trasylol and di-isopropyl...

A comparison of the oximes HS-6 and HI-6 in the therapy of soman intoxication in rodents

The bisquaternary mono-oximes HS-6 and HI-6 may both be considered as potential therapeutic agents for soman intoxication. It has been found that HI-6 was superior to an equal dose of HS-6 in the treatment of soman intoxication in mice and rats. Not only did HI-6 protect against higher levels of soman, but also fewer 'delayed deaths' were seen...

Ca binding of rat fibrinogen and fibrin(ogen) degradation products

The authors studied the binding of Ca to rat fibrinogen and plasmic fibrin(ogen) degradation products by means of equilibrium dialysis with special reference to the protective effect of Ca2+ in the plasmic degradation of fibrinogen. Direct binding studies demonstrate that rat fibrinogen and the plasmic degradation products D(cate) and D-dimer...

Extrapulmonary colony formation after intravenous injection of tumour cells into heparin treated animals

Recent data on extrapulmonary colony formation after heparin administration are inconclusive. A systemic study of this topic was undertaken with 4 experimental tumour systems and 2 distinct periods of reduced clotting capacity in rats and mice. I.v. injection of various numbers of tumour cells into i.p. heparinized animals leads to: Significant...