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Wade, D.R. (author), Lohman, P.H.M. (author), Mattern, I.E. (author), Berends, F. (author), Medisch Biologisch Laboratorium TNO (author)A commercial sample of the tuberculostatic drug isoniazid (INH) was found to have a weak mutagenic activity towards Salmonella typhimurium strains TA100 and TA1535. The addition of a rat or mouse liver homogenate to the test system decreased the mutagenic effect of INH. Hydrazine, an impurity of the INH sample, was also weakly mutagenic in...article 1981
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Creemers, P. (author), Radiobiologisch Instituut TNO (author)Sera from normal mice and mice bearing murine mammary tumor virus (MTV)-induced tumors showed cytotoxic reactions to the MTV-producing Mm5mt/cl cell line. The reaction could be blocked by the addition of MTV, but not of purified gp52 and p28. In sera from tumor bearers, cytotoxic responses ranged from 15 to 66%; reactivity was generally highest...article 1981
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Vink, G.J. (author), Davis, P.B. (author), Hooftman, R.N. (author), Mattern, I.E. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: aroclor, 12767-79-2; benzo[a]pyrene, 50-32-8; mesylic acid ethyl ester, 62-50-0; mesylic acid methyl ester, 66-27-3; triaziquone, 68-76-8article 1981
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Hooisma, J. (author), de Krijger, J. (author), de Groot, D.M.G. (author), Medisch Biologisch Laboratorium TNO (author)Previously tropic effects of extracts from whole chick embryos and from innervated muscles on cultured muscle cells were described. The present study demonstrated similar effects of extracts from 10-days denervated chick muscles. Extracts from innervated as well as from denervated muscles exsanguinated in vivo with saline prior to dissection...article 1981
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Hagenbeek, A. (author), Martens, A.C.M. (author), Radiobiologisch Instituut TNO (author)Results of three different regimens of extracorporeal irradiation of the blood (ECIB) are compared (1 X 20,2 X 10, and 4 X 5hr) in a rat model for human acute myelocytic leukemia. The D0 for X rays of leukemic cells in the blood was 68 cGy. The transit dose varied from 380 to 660 cGy. Evaluation was performed by follow-up of the number of...article 1981
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Wolthuis, O.L. (author), Benschop, H.P. (author), Berends, F. (author), Medisch Biologisch Laboratorium TNO (author)Anaesthetized, atropinized rats were posioned with 6x LD50 soman (1,2,2-trimethylpropyl methylphosphonofluoridate). Purified acetylcholinesterase, injected i.v. 75 min later, was rapidly inhibited, presumably by soman stored in a 'depot' from which it was gradually released. Existence of a depot is supported by the effect of a soman-simulator (...article 1981
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Wolthuis, O. (author), Vanwersch, R.A.P. (author), van der Wiel, H.J. (author), Medisch Biologisch Laboratorium TNO (author)Previous results had shown that bis-pyridinium oximes, particularly HI-6 are quite effective therapeutically in soman-poisoned rats and mice in vivo and in the rat diaphragm preparation in vitro. The aim of the present study was to investigate the efficacy of bis-pyridinium oximes on soman-inhibited neuromuscular transmission in muscle...article 1981
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Medisch Biologisch Labortorium TNO (author), Polak, R.L. (author), Sellin, L.C. (author), Thesleff, S. (author)The acetylcholine (ACh) content and spontaneous and evoked release of ACh in rat extensor digitorum longus (EDL) muscles were determined by pyrolysis mass fragmentography. The determinations were made on muscles paralysed by local application of botulinum toxin (BoTx) type A, on unpoisoned muscles, surgically denervated or reinnervated muscles....article 1981
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Centraal Instituut voor Voedingsonderzoek TNO (author), Spit, B.J. (author), Wibowo, A.A.E. (author), Ferron, V.J. (author), Zielhuis, R.L. (author)Electron microscopical studies were carried out on the kidneys of rabbits given s.c. injections of 0 (control), 0.25 or 0.50 mg lead acetate/kg b.w. 3 times a week during 14 weeks. At the end of the experimental period the animals had lead blood levels of 60, 500 and 600 ??g/1 whole blood respectively. Treatment-related renal changes were found...article 1981
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Bleichrodt, J.F. (author), Roos-Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)Under conditions where the reversion of an amber mutant of bacteriophage ?? by ??-rays is enhanced by subjecting the irradiated phage to SOS repair, ??-ray-induced reversion of two T7 ambers is not influenced by this error-prone bacterial repair system. The survival of T7 ??-irradiated under anoxic conditions is somewhat enhanced by SOS repair,...article 1980
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Vos, O. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: cysteine, 4371-52-2, 52-89-1, 52-90-4; mercaptamine, 156-57-0, 60-23-1; n (1 adamantylmethyl)thioglycolamidine, 60833-81-0article 1980
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- Grollé, T. (author), Kuiper, J. (author), Hoofdgroep Maatschappelijke Technologie TNO (author) article 1980
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- Bol, J. (author), van Overeem, E.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) article 1980
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Wade, M.H. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)The role of the pyrimidine dimer in cell killing, DNA synthesis and repair has been studied by utilizing the light-requiring DNA-repair mechanism of photo-reactivation in UV-irradiated chicken-embryo fibroblasts. Survival, as measured by colony-forming ability at 41??C, is increased in cells left in the light. The initial inhibition of DNA...article 1980
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Vos, O. (author), Luiten, F. (author), Ploemacher, R.E. (author), Medisch Biologisch Laboratorium TNO (author)Treatment of mice with high doses of endotoxin (ET) affected the CFU(s) population in bone marrow, spleen and liver. By 7 days after injection the number of CFU(s) in bone marrow had decreased and the number in spleen and liver had increased. The experimental data show that if mice were irradiated and reconstituted with bone marrow 7 days after...article 1980
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Knook, D.L. (author), Sleyster, E.C. (author), Teutsch, H.F. (author), Instituut voor Experimentele Gerontologie TNO (author)Chemicals/CAS: glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; Glucosephosphate Dehydrogenase, EC 1.1.1.49article 1980
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Plooy, A.C.M. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Ten platinum (Pt) coordination complexes with different ligands, comprising both Pt(II) and Pt(IV) complexes of which the cis-compounds all possessed at least some anti-tumour activity and the trans-compounds were inactive, were tested as to their effect on cell survival and the induction and repair of DNA damage in cultured Chinese hamster...article 1980
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- Feron, V.J. (author), van den Heuvel, P.D. (author), Koeter, H.B.W.M. (author), Beems, R.B. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) article 1980
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de Koning-Verest, I.F. (author), Medisch Biologisch Laboratorium TNO (author)The metabolism of glutamate, taken as an index of the metabolic state of the brain, was studied in brains of 3-, 12- and 30-month-old rats. Following the injection of a mixture of [3H]acetate and d-[2-14C]glucose, the brain levels of glutamate and aspartate were decreased in 30-month-old rats when compared with those of 3-month-old rats. No...article 1980
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Gaubius instituut TNO (author), Kempen, H.J.M. (author)Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1-3 hr) incubation periods. The heaptocytes release very low density (91.10 g/ml). These particles with higher densities have not been positively identified; the latter group is dissimilar to the high density lipoprotein, which occurs in rat liver...article 1980