Searched for: subject%3A%22Enzyme%255C%2Bstability%22
(1 - 16 of 16)
document
Kaper, T. (author), Leemhuis, H. (author), Uitdehaag, J.C.M. (author), van der Veen, B.A. (author), Dijkstra, B.W. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
Glycoside hydrolase family 77 (GH77) belongs to the α-amylase superfamily (Clan H) together with GH13 and GH70. GH77 enzymes are amylomaltases or 4-α-glucanotransferases, involved in maltose metabolism in microorganisms and in starch biosynthesis in plants. Here we characterized the amylomaltase from the hyperthermophilic bacterium Thermus...
article 2007
document
de Groot, J. (author), Kosters, H.A. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
To study the influence of the carbohydrate-moiety of ovalbumin on the formation of the heat-stable conformer S-ovalbumin, ovalbumin is deglycosylated with PNGase-F under native conditions. Although the enzymatic deglycosylation procedure resulted in a complete loss of the ability to bind to Concavalin A column-material, only in about 50% the...
article 2007
document
TNO Kwaliteit van Leven (author), Barends, T.R.M. (author), Bultema, J.B. (author), Kaper, T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), Dijkstra, B.W. (author)
Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent...
article 2007
document
Parnham, M.J. (author), Bogaards, J.J.P. (author), Schrander, F. (author), Schut, M.W. (author), Orešković, K. (author), Mildner, B. (author), TNO Kwaliteit van Leven (author)
PLD-118 is a novel, oral antifungal drug, under development for the treatment of Candida infections. Possible metabolism of PLD-118 by rat, dog and human S9 liver homogenates and inhibition of human cytochrome P450 (CYP) enzymes were investigated. PLD-118 (10 and 100 μm) incubated for 0-60 min with S9 fractions and NADPH was determined by HPLC,...
article 2005
document
van der Veen, M. (author), Norde, W. (author), Stuart, M.C. (author)
The influence of succinylation on lysozyme is studied using circular dichroism, fluorescence spectroscopy, and differential scanning calorimetry. The spectroscopic data reveal that at room temperature the structures of succinylated lysozyme and native lysozyme are similar. However, the calorimetric results show that the thermal stability of...
article 2005
document
TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Euverink, G.J.W. (author), Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D structure [Meng, G. and...
article 2005
document
TNO Kwaliteit van Leven TNO Voeding (author), Kaper, T. (author), Talik, B. (author), Ettema, T.J. (author), Bos, H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Amylomaltases are 4-alpha-glucanotransferases (EC 2.4.1.25) of glycoside hydrolase family 77 that transfer alpha-1,4-linked glucans to another acceptor, which can be the 4-OH group of an alpha-1,4-linked glucan or glucose. The amylomaltase-encoding gene (PAE1209) from the hyperthermophilic archaeon Pyrobaculum aerophilum IM2 was cloned and...
article 2005
document
Gaubius Instituut TNO (author), Walker, J.B. (author), Hughes, B. (author), James, I. (author), Haddock, P. (author), Kluft, C. (author), Bajzar, L. (author)
Two competitive inhibitors of TAFIa (activated thrombin-activable fibrinolysis inhibitor), 2-guanidinoethyl-mercaptosuccinic acid and potato tuber carboxypeptidase inhibitor, variably affect fibrinolysis of clotted human plasma. Depending on their concentration, the inhibitors shortened, prolonged, or had no effect on lysis in vitro. The...
article 2003
document
Wolken, W.A.M. (author), van Loo, W.J.V. (author), Tramper, J. (author), van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A novel lyase, combining hydratase and aldolase activity, that converts citral into methylheptenone and acetaldehyde, was purified from spores of Penicillium digitatum. Remarkably, citral lyase activity was induced 118-fold by incubating nongerminating spores with the substrate, citral. This cofactor independent hydratase/aldolase, was purified...
article 2002
document
van der Maarel, M.J.E.C. (author), van der Veen, B. (author), Uitdehaag, J.C.M. (author), Leemhuis, H. (author), Dijkhuizen, L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Starch is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. A large-scale starch processing industry has emerged in the last century. In the past decades, we have seen a shift from the acid hydrolysis of starch to the use of starch-converting enzymes in the production of maltodextrin,...
article 2002
document
Kronenburg, N.A.E. (author), de Bont, J.A.M. (author), Fischer, L. (author), TNO Milieu, Energie en Procesinnovatie (author)
The yeast Rhodotorula glutinis contains an enantioselective, membrane-associated epoxide hydrolase (EH). Partially purified EH was immobilized in a two-step procedure. In the first step, the proteins were derivatized with itaconic anhydride. In the second step, the derivatized proteins were co-polymerized with ethylene glycol dimethacrylate in...
article 2001
document
Renwick, A.B. (author), Watts, P.S. (author), Edwards, R.J. (author), Barton, P.T. (author), Guyonnet, I. (author), Price, R.J. (author), Tredger, J.M. (author), Pelkonen, O. (author), Boobis, A.R. (author), Lake, B.G. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)
The maintenance of the major hepatic cytochrome P450 (CYP) enzymes has been studied in precision-cut human liver slices cultured for up to 72 h in supplemented RPMI 1640 medium. The relative apoprotein levels of 11 CYP enzymes were determined using a panel of antipeptide antibodes. In addition, 7-ethoxyresorufin O-deethylase, tolbutamide...
article 2000
document
Pots, A.M. (author), Gruppen, H. (author), van Diepenbeek, R. (author), van der Lee, J.J. (author), van Boekel, M.A.J.S. (author), Wijngaards, G. (author), Voragen, A.G.J. (author)
The content and biological activity of patatin and the protease inhibitors of molecular size 20-22 kDa present in whole potato tubers were investigated as a function of storage time. The amount of buffer-extractable protein decreased gradually during storage of whole potatoes of the cultivars Bintje and Desiree for 47 weeks whereas, for Elkana,...
article 1999
document
Pots, A.M. (author), de Jongh, H.H.J. (author), Gruppen, H. (author), Hamer, R.J. (author), Voragen, A.G.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
This paper presents a first structural characterization of isolated patatin, the major potato tuber protein, at ambient and elevated temperatures. Isolated patatin at room temperature is a highly structured molecule at both secondary and tertiary levels. It is estimated from far-ultraviolet circular dichroism data that about 33% of the residues...
article 1998
document
Withers, J.M. (author), Swift, R.J. (author), Wiebe, M.G. (author), Robson, G.D. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
When grown on a medium containing 5 g maltodextrin L-1, Aspergillus niger transformant N402[pAB6-10]B1, which has an additional 20 copies of the glucoamylase (glaA) gene, produced 320 ± 8 mg (mean ± S.E.) glucoamylase (GAM) L-1 in batch culture and 373 ± 9 mg GAM L-1 in maltodextrin- limited chemostat culture at a dilution rate of 0.13 h-1....
article 1998
document
Somers, W.A.C. (author), Koenen, P.H.M. (author), Rozie, H.J. (author), Visser, J. (author), Rombouts, F.M. (author), van 't Riet, K. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The isolation and purification of α-amylase from an industrial enzyme sample is described using crosslinked starch powder as an affinity adsorbent. The process was studied with regard to the stability of the adsorbent, the stability of the enzyme, and the capacity of the adsorbent for the enzyme. The adsorbent was used in a repeated adsorption...
article 1995
Searched for: subject%3A%22Enzyme%255C%2Bstability%22
(1 - 16 of 16)