Nature and histogenesis of sulfite-induced gastric lesions in rats

A variety of gastric lesions can be induced in rats by sodium metabisulfite, including the formation of scattered hyperplastic glands in the fundic mucosa which are exclusively lined by large uniform cells, laden with acidophilic granules. Light and electron microscopy, as enzyme histochemistry, showed that the cells lining these glands are...

Immunoelectronmicroscopy of the binding of high density lipoprotein apoproteins to normal and hypercholesterolemic human fibroblasts

We visualized the apolipoproteins of high density lipoproteins (HDL) that were bound to cultured human fibroblasts at the ultrastructural level, with an immunoenzymecytochemical method. By using antisera against (a) apolipoprotein AI (a major apolipoprotein of HDL) and (b) apolipoprotein E (an apolipoprotein present in HDL), the binding of HDL...

Ultrastructure of hepatic angiosarcoma in rats induced by vinyl chloride

The hepatic angiosarcoma studied was from a male Wistar rat having been exposed to vinyl chloride monomer (VCM) by the oral route for 120 weeks. Widened sinusoids lined by large electron-lucent, spindle-shaped tumor cells and membrane-bound nuclear-free structures were seen in the transitional zone between the tumor mass and the adjacent liver...

Binding of LP(a) to the low density lipoprotein receptor of human fibroblasts

Chemicals/CAS: Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDL

Onderzocht en toegepast: 14 opstellen over problemen van waarnemen, beslissen en handelen in de dagelijkse praktijk, uitgegeven door het Instituut voor Zintuigfysiologie TNO t.g.v. het afscheid van de direkteur Dr.ir. P.L. Walraven

Ultrastructural changes in the kidneys of rabbits treated with lead acetate

Electron microscopical studies were carried out on the kidneys of rabbits given s.c. injections of 0 (control), 0.25 or 0.50 mg lead acetate/kg b.w. 3 times a week during 14 weeks. At the end of the experimental period the animals had lead blood levels of 60, 500 and 600 ??g/1 whole blood respectively. Treatment-related renal changes were found...

Stereological analysis of liver parenchymal cells from young and old rats

Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of the liver lobule, while the...

Recent developments in permanent magnet focusing assemblies for image intensifiers and camera tubes

Structural identity of the pluripotential hemopoietic stem cell

A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. This entity was subsequently discovered in concentrated HSC preparations from bone marrow of rats, monkeys, and humans. In the mouse, a set of physical...

A low frequency meter with instantaneous response

A practical method is described for instantaneous frequency measurement by computation of the reciprocal of the time interval between two events. Use is made of inexpensive integrated circuits.

The cellular basis of organ ageing

Ageing is associated with declines in the functional capacities of several organs. General causes for the decline can be divided into: 1. intrinsic cellular causes and 2. extracellular causes, e.g., changes in blood circulation and distribution. For the first group of causes, there is evidence for a decrease in the numbers of long-lived cells in...

Mutagenicity of ethidium bromide after metabolic activation in vitro

Subcellular localization of newly formed [3H]acetylcholine in rat cerebral cortex in vitro

Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...

Elektronenmikroskopische Untersuchung von Asbestkörperchen und Asbestfasern in der Lunge eines Mesotheliom-Patienten [Investigation of asbestos bodies and asbestos fibers found in the lungs of a mesothelioma patient by electron microscopy]

Asbestos bodies and asbestos fibers were found in the lungs of a mesothelioma patient and analysed by electron microscopy. The bodies were normal asbestos bodies with amphibole-asbestos fibers as kernels. The free asbestos fibers, present in the lungs, mainly were chrysotile-asbestos. © 1973 Springer-Verlag. Chemicals/CAS: asbestos, 1332-21-4;...

Identification of cells in primate bone marrow resembling the hemopoietic stem cell in the mouse

The colony forming unit culture (CFU C) in the thin layer agar colony technique is considered to be representative for hemopoietic stem cells (HSC), according to studies in mouse and monkey bone marrow. Using this in vitro assay as a guide, stem cell concentrates were prepared from monkey and human bone marrow by repeated density gradient...

Incomplete sedimentation of Rauscher leukaemia virus particles during ultracentrifugation

Micro-elektronica

Ofschoon het publiek gebruik van ruimtevaartuigen op zijn minst nog in het verre verschiet ligt is het wel interessant om zich eens af te vragen wat de kosten daarvan zouden zijn. Zonder enige pretentie van nauwkeurigheid mag het tarief voor het brengen van betalende lading in een baan om de aarde thans op ongeveer 150 gulden per gram gesteld...