Searched for: subject%3A%22Drug%255C%2Bstability%22
(1 - 20 of 23)

Pages

document
Muller, T. (author), Nowak, A. (author), Wiedensohler, A. (author), Sheridan, P. (author), Laborde, M. (author), Covert, D.S. (author), Marinoni, A. (author), Imre, K. (author), Henzing, B. (author), Roger, J.C. (author), Dos Santos, S.M. (author), Wilhelm, R. (author), Wang, Y.Q. (author), de Leeuw, G. (author), TNO Bouw en Ondergrond (author)
Integrating nephelometers are widely used for monitoring and research applications related to air pollution and climate. Several commercial versions of the instrument are available and are in wide use in the community. This article reports on results from a calibration and intercomparison workshop where several units of the three most widely...
article 2009
document
van Boxtel, E.L. (author), van den Broek, L.A.M. (author), Koppelman, S.J. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)
Legumin proteins Ara h 3 from peanuts and glycinin from soybeans are increasingly described as important allergens. The stability of an allergen's IgE binding capacity towards heating and digestion is considered an important characteristic for food allergens. We investigated the effects of heating and digestion on the IgE binding of Ara h 3 and...
article 2008
document
Dihal, A.A. (author), Tilburgs, C. (author), van Erk, M.J. (author), Rietjens, I.M.C.M. (author), Woutersen, R.A. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)
The aim was to investigate mechanisms contributing to quercetin's previously described effects on cell-proliferation and -differentiation, which contradicted its proposed anticarcinogenic potency. In a 10-day experiment, 40 μM quercetin stabilized by 1 mM ascorbate reduced Caco-2 differentiation up to 50% (p < 0.001). Caco-2 RNA from days 5 and...
article 2007
document
Wierenga, P.A. (author), Egmond, M.R. (author), Voragen, A.G.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
Unfolding of proteins has often been mentioned as an important factor during the adsorption process at air-water interfaces and in the increase of surface pressure at later stages of the adsorption process. This work focuses on the question whether the folding state of the adsorbed protein depends on the rate of adsorption to the interface,...
article 2006
document
Dihal, A.A. (author), Woutersen, R.A. (author), Ommen, B.v. (author), Rietjens, I.M.C.M. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)
The effect of the dietary flavonoid quercetin was investigated on proliferation and differentiation of the human colon cancer cell line Caco-2. Confluent Caco-2 monolayers exposed to quercetin showed a biphasic effect on cell proliferation and a decrease in cell differentiation (0.001<P<0.05). During differentiation Caco-2 cells formed 5 phase...
article 2006
document
Liebsch, M. (author), Spielmann, H. (author), Pape, W. (author), Krul, C. (author), Deguercy, A. (author), Eskes, C.A.M. (author), TNO Kwaliteit van Leven (author)
Regulatory requirements: According to the current Notes for Guidance of the Scientific Committee on Cosmetic Products and Non-Food Products (SCCNFP), cosmetic ingredients and mixtures of ingredients absorbing UV light (in particular UV filter chemicals used, for example, to ensure the light stability of cosmetics or used in sun protection...
article 2005
document
Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author), TNO Voeding (author)
The thermal unfolding of potato serine protease inhibitor (PSPI), the most abundant protease inhibitor group in potato tuber, was measured using far UV CD spectroscopy, fluorescence spectroscopy, and DSC. The results indicate that the thermal as well as the guanidinium-induced unfolding of PSPI occurs via a non-two-state mechanism in which at...
article 2005
document
de Visser, P.C. (author), van Hooft, P.A.V. (author), de Vries, A.-M. (author), de Jong, A. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Noort, D. (author), TNO Defensie en Veiligheid (author)
An array of analogues of the cationic antimicrobial peptide drosocin was synthesized containing substitutions of Tyr6 and Ser7 in order to increase the proteolytic stability. Stabilizing the N-terminus with unnatural amino acids increased the serum stability of analogues by almost a factor 30 over an 8 h period. © 2005 Elsevier Ltd. All rights...
article 2005
document
de Visser, P.C. (author), Govaerts, C. (author), van Hooft, P.A.V. (author), Overkleeft, H.S. (author), van Schepdael, A. (author), Hoogmartens, J. (author)
In the course of the synthesis and purification of new polymyxins and analogues, formation of a by-product with identical mass was observed and it was believed that this might be the result of acyl migration from the Nα- to the Nγ-position of residue α,γ-diaminobutyric acid 1 (Dab1) under acidic conditions. Therefore, a LC-MS/MS study was...
article 2004
document
Centraal Instituut voor Voedingsonderzoek TNO (author), Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G.A. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author)
Potato serine protease inhibitor (PSPI) is the most abundant protease inhibitor group in potato tuber. The investigated PSPI isoforms have a highly similar structure at both the secondary and the tertiary level. From the results described, PSPI is classified as a β-II protein based on (1) the presence in the near-UV spectra of sharp peaks,...
article 2004
document
Kosters, H.A. (author), Broersen, K. (author), de Groot, J. (author), Simons, J.W.F.A. (author), Wierenga, P. (author), de Jongh, H.H.J. (author), TNO Voeding (author)
Processing of ovalbumin may result in proteins that differ more than 23°C in denaturation temperature while the structural fold is not significantly affected. This is achieved by 1) conversion of positive residues into negative ones (succinylation); 2) elimination of negative charges (methylation); 3) reducing the proteins hydrophobic exposure ...
article 2003
document
TNO Voeding (author), Lakemond, C.M.M. (author), de Jongh, H.H.J. (author), Gruppen, H. (author), Voragen, A.G.J. (author)
In heat denaturation studies conducted in the past the genetic variants of glycinin have been considered as a homogeneous group of proteins. In this work the validity of this assumption was tested. It was found by calorimetric studies that glycinin denatures heterogeneously at pH 7.6. When the temperature of isothermal treatment is increased...
article 2002
document
Goedde, M.F. (author), Grimbergen, J.M. (author), Toet, K.H. (author), Sitter, T. (author), Quax, P.H. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
Background. The mesothelium has an important role in maintaining an adequate fibrinolytic capacity in the peritoneal cavity and thus in preventing the formation of fibrinous peritoneal adhesions by secreting the fibrinolytic enzyme tissue-type plasminogen activator (t-PA). The fibrinolytic activity of human mesothelial cells (HMCs) is...
article 2001
document
Spruit, W.E.T. (author), Trap, H.C. (author), Langenberg, J.P. (author), Benschop, H.P. (author)
article 2001
document
Rijken, D.C. (author), Groeneveld, E. (author), Barrett-Bergshoeff, M.M. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)
BM 06.022 is a non-glycosylated mutant of human tissue-type plasminogen activator (t-PA) comprising only the kringle-2 and proteinase domains. The in vivo half-life of BM 06.022 antigen is 4- to 5-fold longer than that of t-PA antigen. The in vitro half-life of the activity of BM 06.022 at therapeutic concentrations in plasma is shorter than...
article 1994
document
Centraal Instituut voor Voedingsonderzoek TNO (author), Lina, B.A.R. (author), Dreef-Van Der Meulen, H.C. (author), Leegwater, D.C. (author)
Neohesperidin dehydrochalcone was administered to groups of 20 male and 20 female Wistar rats at dietary levels of 0, 0.2, 1.0 and 5.0% for 91 days. No treatment-related ophthalmoscopical, haematological or histopathological effects were observed. In the high-dose group, a marked caecal enlargement occurred in both sexes, accompanied by soft...
article 1990
document
Speek, A.J. (author), van Agtmaal, E.J. (author), Saowakontha, S. (author), Schreurs, W.H.P. (author), van Haeringen, N.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 ??l human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A...
article 1986
document
Speek, A.J. (author), Wongkham, C. (author), Limratana, N. (author), Saowakontha, S. (author), Schreurs, W.H.P. (author), Instituut CIVO-Toxicologie en voeding TNO (author)
Chemicals/CAS: n,n dimethylacetamide, 127-19-5; retinol, 68-26-8, 82445-97-4; Vitamin A, 11103-57-4
article 1986
document
van Eeden, C.H. (author), de Jong, A.W.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The well-known Kroller method for the determination of cyanide requires the use of the harmful chemicals benzidine (carcinogenic) and bromine (toxic) in the colorimetric part; the analytical result depends strongly on the conduct of the liberation procedure. The substitution of barbituric acid and N-chlorosuccinimide for benzidine and bromine...
article 1985
document
Tas, A.C. (author), Odink, J. (author), Ten Noever De Brauw, M.C. (author), Schrijver, J. (author), Jonk, R.J.G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A method has been developed for the derivatization of both catecholamines (dopamine, noradrenaline and adrenaline) and their 3-O-methylated metabolites (3-methoxytyramine, normetanephrine and metanephrine) in a single run. The compounds were first incubated with methanolic hydrochloric acid to methylate those compounds that contain a benzylic...
article 1984
Searched for: subject%3A%22Drug%255C%2Bstability%22
(1 - 20 of 23)

Pages