A method for determination of acetylcholine by slow pyrolysis combined with mass fragmentography on a packed capillary column

A new procedure is described for the estimation of ACh by pyrolysis-gas chromatography/mass spectrometry. ACh iodide and the iodides of ACh-d16 or propionylcholine are slowly demethylated at 250??C by pyrolysis on the tip of a glass probe after insertion into the heated entrance of a glass tube leading to a packed capillary column. The volatile...