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van Oosten, J.J.M. (author), Bakker, E. (author), TNO Wegtransportmiddelen (author)Tyre behaviour plays an important role in vehicle dynamics studies. The Magic Formula Tyre Model is a semi-empirical tyre model which can describe tyre behaviour quite accurately. A set of Magic Formula parameters constitutes the basis of the model. The determination of these parameters out of tyre measurement data produced by the Delft tyre...article 1991
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Schielen, W.J.G. (author), Adams, H.P.H.M. (author), van Leuven, K. (author), Voskuilen, M. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)Fibrin accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rate-enhancing effect of fibrin is brought about by two sites in the fibrin molecule: one in Aα-(148-160) and one in the γ-chain stretch 311-379 (also known as FCB-5). During...article 1991
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Gaubius instituut TNO (author), Schielen, W.J.G. (author), Adams, H.P.H.M. (author), Voskuilen, M. (author), Tesser, G.J. (author), Nieuwenhuizen, W. (author)The sequence fibrinogen-Aα-(148-160) can mimic part of the fibrin-induced rate enhancement of the activation of plasminogen by tissue-type plasminogen activator. Previously we have reported that the lysine residue at position Aα-157 is crucial. During our further investigations on Aα-157 we found that lysine at position Aα-157 may be replaced by...article 1991
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Rijken, D.C. (author), Groeneveld, E. (author), Gaubius Instituut TNO (author)Recent studies suggest that plasminogen activators not only hydrolyse a specific arginine-valine bond in plasminogen, but may also cleave other proteins such as fibronectin. We studied the substrate specificity, particularly the preference for arginyl over lysyl peptide bonds, of tissue-type plasminogen activator (t-PA) as well as of two-chain...article 1991
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Punt, P.J. (author), Zegers, N.D. (author), Busscher, M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of Aspergillus nidulans. Both the homologous gpdA and the heterologous Escherichia coli β-galactosidase ...article 1991
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Gaubius Institute TNO (author), Koopman, J. (author), Haverkate, F. (author), Briet, E. (author), Lord, S.T. (author)A congenitally abnormal fibrinogen (Vlissingen) was isolated from the blood of a young woman suffering from massive pulmonary embolism. Fibrinogen Vlissingen showed an abnormal clotting time with both thrombin and Reptilase(TM). The release of the fibrino-peptides A and B by thrombin was normal, but fibrin polymerization was impaired both in the...article 1991
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- Nederlands Instituut voor Praeventieve Gezondheidszorg TNO TNO Preventie en Gezondheid (author), Verrips, G.H. (author) report 1991
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Fysisch en Elektronisch Laboratorium TNO (author), van Oosterom, P.J.M. (author)This paper presents the first fully dynamic and reactive data structure. Reactive data structures are vector based structures tailored to the efficient storage and retrieval of geometric objects at different levels of detail. Geometric selections can be interleaved by insertions of new objects and by deletions of existing objects. The core of...conference paper 1991
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- Weening-Verhoeff, E.J.D. (author), Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Rehberg, E.F. (author), Marotti, K.R. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author) article 1990
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Schielen, W.J. (author), Voskuilen, M. (author), Adams, P.J. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)Fibrin, not fibrinogen, enhances the rate of tPA catalysed plasminogen activation. In earlier studies we have shown that a site involved in this rate enhancement is located in a tridecapeptide, i.e. fibrinogen A alpha-(148-160). This sequence comprises a special charge distribution in which a stretch with alternating neutral and acidic amino...article 1990
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Burger, H. (author), van Leen, R.W. (author), Dorssers, L.C.J. (author), Persoon, N.L.M. (author), Lemson, P.J. (author), Wagemaker, G. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)To enable preclinical studies on homologous interleukin-3 (IL-3) in primate species, we isolated the gene encoding Rhesus monkey IL-3 (RhIL-3). The nucleotide sequence of the RhIL-3 gene displayed 92.9% homology with that of the human IL-3 (hIL-3) gene. The isolated RhIL-3 gene encodes a 143-amino acid (aa) precursor polypeptide, nine C-terminal...article 1990
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Medisch Biologisch Laboratorium TNO (author), Punt, P.J. (author), Dingemanse, M.A. (author), Kuyvenhoven, A. (author), Soede, R.D.M. (author), Pouvels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Analysis of the promoter region of the highly expressed Aspergillus nidulans gpdA gene is described. The nucleotide (nt) sequence of a 1.3-kb region upstream from the ATG was determined. Comparison with promoter regions of other Aspergillus and Neurospora genes revealed several regions similar sequence. Both random and site-specific mutations...article 1990
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van Gorcom, R.F.M. (author), Boschloo, J.G. (author), Kuijvenhoven, A. (author), Lange, J. (author), van Vark, A.J. (author), Bos, C.J. (author), van Balken, J.A.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)The gene coding for benzoate-para-hydroxylase (bphA) of Aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. The nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcription start and termination sites were...article 1990
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van der Horst, A.R.A. (author), Godthelp, J. (author), Instituut voor Zintuigfysiologie TNO (author)The limitations of human information processing capabilities are apparent in modern road traffic. Research methods for studying road user behavior range from unobtrusive observations of actual traffic to highly controlled laboratory experiments. Often the incompatibility between controllability and validity makes the choice of a suitable method...conference paper 1989
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Medisch Biologisch Laboratorium TNO (author), van Noort, J.M. (author), van der Drift, A.C.M. (author)The selectivity of cathepsin D, a mammalian intracellular aspartyl proteinase involved in the degradation of endocytosed proteins, was studied. For this purpose, several proteins of known primary structure were subjected to mild proteolysis by the enzyme, and the preferentially cleaved peptide bonds were identified. Comparison of the primary...article 1989
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Fysisch en Elektronisch Laboratorium TNO (author), Joppe, J.L. (author)Door ontwikkelingen als de digitale (high definition) televisie zal de behoefte aan breedbandig datatransport sterk toenemen. Optische communicatie is bij uitstek geschikt om deze functie te vervullen. Het Fysisch en Elektrontsch Laboratorium TNO (FEL-TNO) draagt door onderzoek naar de koppeling van een laser en een optische schakeling bij aan...article 1989
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Fysisch en Elektronisch Laboratorium TNO (author), van Oosterom, P.J.M. (author)We introduce a Reactive Data Structure, that is a spatial data structure with detail levels. The two properties, spatial organization and detail levels, are the basis for a Geographic Information System with a multi-scale database. A reactive data structure is a novel type of data structure catering to S. It is presented here as a modification...conference paper 1989
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Gaubius Instituut TNO (author), Rijken, D.C. (author), Groeneveld, E. (author), Kluft, C. (author), Nieuwenhuis, H.K. (author)α2-Antiplasmin Enschede is a variant of α2-antiplasmin which has lost its ability to inhibit plasmin irreversibly and which is associated with haemorrhagic disorder [Kluft et al. (1987) J. Clin. Invest. 80, 1391-1400]. The abnormal protein was purified from the plasma of a homozygous patient and subjected to one-dimensional peptide mapping using...article 1988
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Gaubius Institute TNO, 2313 AD Leiden, Netherlands (author), Bosma, P.J. (author), van den Berg, E.A. (author), Kooistra, T. (author), Siemieniak, D.R. (author), Slightom, J.L. (author)We have determined the nucleotide sequence of the human plasminogen activator inhibitor-1 (PAI-1) gene and significant stretches of DNA which extend into its 5'- and 3'-flanking DNA regions; a total sequence of 15,867 base pairs (bp) is presented. The sequenced 5'-flanking DNA (1,520 bp) contains the essential eukaryotic cis-type proximal...article 1988
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Medisch Biologisch Laboratorium TNO (author), Kos, T. (author), Kuijvenhoven, A. (author), Hessing, H.G.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Molecular Sequence Numbers: GENBANK: X07071; Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; DNA Restriction Enzymes, EC 3.1.21; Tryptophan, 73-22-3article 1988