Searched for: subject:"Culture%5C+Techniques"
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Treijtel, N. (author), Eijkeren, J.C.H.v. (author), Nijmeijer, S. (author), van der Greef de - Sandt, I.C.J. (author), Freidig, A.P. (author), TNO Kwaliteit van Leven (author)
The metabolism and active transport of ritonavir and saquinavir were studied using sandwich-cultured rat hepatoyctes and rat liver microsomes. For ritonavir four comparable metabolites were observed in the sandwich-culture and in microsomes. For saquinavir eight metabolites were observed in sandwich-culture and 14 different metabolites in...
article 2009
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Koudstaal, W. (author), Hartgroves, L. (author), Havenga, M. (author), Legastelois, I. (author), Ophorst, C. (author), Siewerts, M. (author), Zuijdgeest, D. (author), Vogels, R. (author), Custers, J. (author), de Boer-Luijtze, E. (author), de Leeuw, O. (author), Cornelissen, L. (author), Goudsmit, J. (author), Barclay, W. (author), TNO Kwaliteit van Leven (author)
Reverse genetics, the generation of influenza viruses from cDNA, presents a rapid method for creating vaccine strains. The technique necessitates the use of cultured cells. Due to technical and regulatory requirements, the choice of cell lines for production of human influenza vaccines is limited. PER.C6® cells, among the most extensively...
article 2009
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), Jansen, K.M.B. (author), Verhaar, J.A.N. (author), de Groot, J. (author), Vanosch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)
To understand cartilage degenerative diseases and improve repair procedures, we investigate the influence of glycosaminoglycans (GAGs) on cartilage matrix biochemistry and functionality. Bovine articular chondrocytes were cultured in alginate beads with(out) para-nitrophenyl-beta-d-xyloside (PNPX) to inhibit GAG incorporation into newly formed...
article 2009
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Arts, J.H.E. (author), Muijser, H. (author), Jonker, D. (author), van de Sandt, J.J.M. (author), Bos, P.M.J. (author), Feron, V.J. (author), TNO Kwaliteit van Leven (author)
The OECD Health Effects Test Guidelines (TGs) provide guidance concerning the use of methods for the identification and characterization of hazards from chemical substances. These TGs are largely based on tests in routine use for many years and are known to yield information relevant to various types of toxicity. They have proven their value in...
article 2008
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Jansen, N.W.D. (author), Roosendaal, G. (author), Bijlsma, J.W.J. (author), de Groot, J. (author), Theobald, M. (author), Lafeber, F.P.J.G. (author), TNO Kwaliteit van Leven (author)
Background: Joint bleeds have a direct adverse effect on joint cartilage, leading to joint deterioration and, ultimately, to disability. Objective: To examine the hypothesis that because degenerated cartilage has a limited repair capacity, it is more susceptible than healthy cartilage to blood-induced cartilage damage. Methods: Healthy,...
article 2008
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Abildayeva, K. (author), Berbée, J.F.P. (author), Blokland, A. (author), Jansen, P.J. (author), Hoek, F.J. (author), Meijer, O. (author), Lütjohann, D. (author), Gautier, T. (author), Pillot, T. (author), de Vente, J. (author), Havekes, L.M. (author), Ramaekers, F.C.S. (author), Kuipers, F. (author), Rensen, P.C.N. (author), Mulder, M. (author), TNO Kwaliteit van Leven (author)
The H2 allele of APOC1, giving rise to increased gene expression of apolipoprotein C-I (apoC-I), is in genetic disequilibrium with the APOE4 allele and may provide a major risk factor for Alzheimer's disease (AD). We found that apoC-I protein is present in astrocytes and endothelial cells within hippocampal regions in both human control and AD...
article 2008
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Verhoef, S. (author), Ruijssenaars, H.J. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
Pseudomonas putida strain S12palB1 was constructed that produces p-hydroxybenzoate from renewable carbon sources via the central metabolite l-tyrosine. P. putida S12palB1 was based on the platform strain P. putida S12TPL3, which has an optimised carbon flux towards l-tyrosine. Phenylalanine ammonia lyase (Pal) was introduced for the conversion...
article 2007
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Bikker, F.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Fidder, A. (author), van der Schans, G.P. (author), TNO Defensie en Veiligheid (author)
As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming...
article 2007
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Lu, Z.F. (author), Zandieh Doulabi, B. (author), Wuisman, P.I. (author), Bank, R.A. (author), Helder, M.N. (author), TNO Kwaliteit van Leven (author)
Degenerative disc disease (DDD) is a major cause of chronic low back pain. For mild/intermediate DDD, regeneration by injecting adipose stem cells (ASCs) into the nucleus pulposus (NP) may be considered. The goal of this study is to investigate whether NP cells can direct ASCs towards the NP phenotype. Interactions between NP cells and ASCs were...
article 2007
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Meijer, M.J. (author), Mieremet-Ooms, M.A.C. (author), van Duijn, W. (author), van der Zon, A.M. (author), Hanemaaijer, R. (author), Verheijen, J.H. (author), van Hogezand, R.A. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), TNO Kwaliteit van Leven (author)
Background: Previous studies have shown an upregulation of matrix metalloproteinases (MMPs) in intestinal tissue of patients with inflammatory bowel disease (IBD) and significant clinical improvement after administration of the anti-TNF-α antibody infliximab. The aims of our study were to determine expression and secretion of MMP-1, -2, -3, -9,...
article 2007
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Guimarães, A.H.C. (author), Laurens, N. (author), Weijers, E.M. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Rijken, D.C. (author), TNO Kwaliteit van Leven (author)
OBJECTIVE - Besides having a key role in fibrinolysis, the plasminogen system has been implicated in cell migration and angiogenesis. A common mechanism is the binding of plasminogen to carboxy-terminal lysine residues in partially degraded fibrin or on cellular surfaces. Here we examined the involvement of thrombin activatable fibrinolysis...
article 2007
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Kienhuis, A.S. (author), Wortelboer, H.M. (author), Hoflack, J.C. (author), Moonen, E.J. (author), Kleinjans, J.C.S. (author), van Ommen, B. (author), van Delft, J.H.M. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)
Sandwich-cultured primary rat hepatocytes are often used as an in vitro model in toxicology and pharmacology. However, loss of liver-specific functions, in particular, the decline of cytochrome P450 (P450) enzyme activity, limits the value of this model for prediction of in vivo toxicity. In this study, we investigated whether a hepatic in vitro...
article 2006
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Kapiteijn, K. (author), Koolwijk, P. (author), van der Weiden, R.M.F. (author), van Nieuw Amerongen, G. (author), Plaisier, M. (author), van Hinsbergh, V.W.M. (author), Helmerhorst, F.M. (author), TNO Kwaliteit van Leven (author)
Objective: Successful implantation and placentation depend on the interaction between the endometrium and the embryo. Angiogenesis is crucial at this time. In this article we investigate the direct influence of the human embryo on in vitro endometrial angiogenesis. Design: In vitro study. Setting: Human endometrial microvascular endothelial...
article 2006
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Lehé, C.L. (author), Jacobs, J.J.L. (author), Hua, C.M. (author), Courtellemont, P. (author), Elliott, G.R. (author), Das, P.K. (author), TNO Defensie en Veiligheid (author)
The accelerated migration of Langerhans cells (LCs) out of the epidermis and up-regulation of maturation markers, upon treatment with subtoxic concentrations of chemicals, were used as the criteria to determine the potential of allergenic chemicals capable of inducing a hapten-specific delayed-type hypersensitivity reaction. Here we report the...
article 2006
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Jacobs, J.J.L. (author), Lehé, C.L. (author), Hasegawa, H. (author), Elliott, G.R. (author), Das, P.K. (author), TNO Defensie en Veiligheid (author)
Skin irritants and contact allergens reduce the number of Langerhans cells (LCs). It has been assumed that this reduction is due their migration to the draining lymph node (LN) for initiating immune sensitization in a host. Skin irritation, however, as opposed to contact allergy is not considered to be an immunological disease. Nevertheless,...
article 2006
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Schepers, A. (author), Eefting, D. (author), Bonta, P.I. (author), Grimbergen, J.M. (author), de Vries, M.R. (author), van Weel, V. (author), de Vries, C.J. (author), Egashira, K. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)
OBJECTIVE - Because late vein graft failure is caused by intimal hyperplasia (IH) and accelerated atherosclerosis, and these processes are thought to be inflammation driven, influx of monocytes is one of the first phenomena seen in IH, we would like to provide direct evidence for a role of the MCP-1 pathway in the development of vein graft...
article 2006
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Zuang, V. (author), Alonso, M.A. (author), Botham, P.A. (author), Eskes, C. (author), Fentem, J. (author), Liebsch, M. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author)
article 2005
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Barbosa, M.J. (author), Zijffers, J.W. (author), Nisworo, A. (author), Vaes, W. (author), van Schoonhoven, J. (author), Wijffels, R.H. (author), TNO Kwaliteit van Leven (author)
Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and...
article 2005
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Verwei, M. (author), van den Berg, H. (author), Havenaar, R. (author), Groten, J.P. (author), TNO Defensie en Veiligheid TNO Kwaliteit van Leven (author)
Background: Milk products are a potential matrix for fortification with synthetic folic acid or natural 5-methyltetrahydrofolate (5-CH 3-H4folate) to enhance the daily folate intake. In milk, folate occurs bound to folatebinding proteins (FBP). Our previous studies with an in vitro gastrointestinal model showed that 70% of the initial FBP...
article 2005
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van Beek, E.R. (author), Cohen, L.H. (author), Leroy, I.M. (author), Ebetino, F.H. (author), Löwik, C.W.G.M. (author), Papapoulos, S.E. (author), Gaubius Instituut TNO (author)
Bisphosphonates (BPS) inhibit bone resorption and are divided into two classes according to their chemical structure and mechanism of action: nonnitrogen containing BPS such as etidronate and clodronate that are of low potency and inhibit osteoclast function via metabolism into toxic ATP-metabolites and nitrogen-containing BPS (NBPS), such as...
article 2003
Searched for: subject:"Culture%5C+Techniques"
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