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TNO Kwaliteit van Leven (author), Verhoef, S. (author), Wierckx, N. (author), Westerhof, R.G.M. (author), Winde, J.H.de (author), Ruijssenaars, H.J. (author)Two solvent-tolerant Pseudomonas putida S12 strains, originally designed for phenol and p-coumarate production, were engineered for efficient production of p-hydroxystyrene from glucose. This was established by introduction of the genes pal and pdc encoding L-phenylalanine/L-tyrosine ammonia lyase and p-coumaric acid decarboxylase, respectively....article 2009
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van Groenestijn, J.W. (author), Geelhoed, J.S. (author), Goorissen, H.P. (author), Meesters, K.P.M. (author), Stams, A.J.M. (author), Claassen, P.A.M. (author), TNO Kwaliteit van Leven (author)Non-axenic operation of a 400 L trickle bed reactor inoculated with the thermophile Caldicellulosiruptor saccharolyticus, yielded 2.8 molH 2mol hexose converted. The reactor was fed with a complex medium with sucrose as the main substrate, continuously flushed with nitrogen gas, and operated at 73°C. The volumetric productivity was 22 mmolH2(L...article 2009
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Shi, Y. (author), Xu, X. (author), Zhu, Y. (author), TNO Kwaliteit van Leven (author)Verticillium lecanii is an entomopathogen with high potential in biological control of pests. We developed a solid-state fermentation with sugarcane bagasse as carrier absorbing liquid medium to propagate V. lecanii spores. Using statistical experimental design, we optimized the medium composition for spore production. We first used one-factor...article 2009
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Lu, Z.F. (author), Doulabi, B.Z. (author), Huang, C.L. (author), Bank, R.A. (author), Helder, M.N. (author), TNO Kwaliteit van Leven (author)β1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that β1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis....article 2008
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TNO Kwaliteit van Leven (author), Spaan, S. (author), Doekes, G. (author), Heederik, D. (author), Thorne, P.S. (author), Wouters, I.M. (author)The measurement of airborne endotoxins is thus far not standardized. Earlier studies reported higher endotoxin yields when Tween 20 was added to the media used for filter extraction and in the Limulus amebocyte lysate (LAL) assay. This study compared four common media and assessed the effects of Tween during extraction and analysis separately....article 2008
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van der Werf, M.J. (author), Pieterse, B. (author), van Luijk, N. (author), Schuren, F. (author), van der Werff van der - Vat, B. (author), Overkamp, K. (author), Jellema, R.H. (author), TNO Kwaliteit van Leven (author)The value of the multivariate data analysis tools principal component analysis (PCA) and principal component discriminant analysis (PCDA) for prioritizing leads generated by microarrays was evaluated. To this end, Pseudomonas putida S12 was grown in independent triplicate fermentations on four different carbon sources, i.e. fructose, glucose,...article 2006
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Kapiteijn, K. (author), Koolwijk, P. (author), van der Weiden, R.M.F. (author), van Nieuw Amerongen, G. (author), Plaisier, M. (author), van Hinsbergh, V.W.M. (author), Helmerhorst, F.M. (author), TNO Kwaliteit van Leven (author)Objective: Successful implantation and placentation depend on the interaction between the endometrium and the embryo. Angiogenesis is crucial at this time. In this article we investigate the direct influence of the human embryo on in vitro endometrial angiogenesis. Design: In vitro study. Setting: Human endometrial microvascular endothelial...article 2006
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TNO Kwaliteit van Leven (author), van Wezel, G.P. (author), Krabben, P. (author), Traag, B.A. (author), Keijser, B.J.F. (author), Kerste, R. (author), Vijgenboom, E. (author), Heijnen, J.J. (author), Kraal, B. (author)Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the...article 2006
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Werlen, C. (author), Jaspers, M.C.M. (author), van der Meer, J.R. (author)Genetically constructed microbial biosensors for measuring organic pollutants are mostly applied in aqueous samples. Unfortunately, the detection limit of most biosensors is insufficient to detect pollutants at low but environmentally relevant concentrations. However, organic pollutants with low levels of water solubility often have significant...article 2004
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Collen, A. (author), Hanemaaijer, R. (author), Lupu, F. (author), Quax, P.H.A. (author), van Lent, N. (author), Grimbergen, J. (author), Peters, E. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)Adult angiogenesis, associated with pathologic conditions, is often accompanied by the formation of a fibrinous exudate. This temporary matrix consists mainly of fibrin but is intermingled with plasma proteins and collagen fibers. The formation of capillary structures in a fibrinous matrix in vivo was mimicked by an in vitro model, in which...article 2003
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Malakar, P.K. (author), Barker, G.C. (author), Zwietering, M.H. (author), van 't Riet, K. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Microbial interaction can be ignored in predictive microbiology under most conditions. We show that interactions are only important at high population densities, using published data on inhibition of growth of Listeria monocytogenes in broth. Our analysis using growth models from predictive microbiology indicated that interactions only occur at...article 2003
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de Jong, A.E.I. (author), Eijhusen, G.P. (author), Brouwer-Post, E.J.F. (author), Grand, M. (author), Johansson, T. (author), Kärkkäinen, T. (author), Marugg, J. (author), in 't Veld, P.H. (author), Warmerdam, F.H.M. (author), Wörner, G. (author), Zicavo, A. (author), Rombouts, F.M. (author), Beumer, R.R. (author)Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens (SFP) agar, sulfite cycloserine azide (SCA), differential clostridial agar (DCA), and oleandomycin polymyxin sulfadiazine perfringens (OPSP) agar]...article 2003
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Heikkilä, P. (author), Teronen, O. (author), Hirn, M.Y. (author), Sorsa, T. (author), Tervahartiala, T. (author), Salo, T. (author), Konttinen, Y.T. (author), Halttunen, T. (author), Moilanen, M. (author), Hanemaaijer, R. (author), Laitinen, M. (author)Background. Bisphosphonates reduce the bone metastasis formation and angiogenesis but the exact molecular mechanisms involved are unclear. Progelatinase A (proMMP-2; 78 KDa) is activated up during the tumor spread and metastasis by a cell surface-associated matrix metalloproteinase (membrane-type matrix metalloproteinase [MT1-MMP] or MMP-14)....article 2003
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Lamfers, M.L.M. (author), Grimbergen, J.M. (author), Aalders, M.C. (author), Havenga, M.J. (author), de Vries, M.R. (author), Huisman, L.G.M. (author), van Hinsbergh, V.W.M. (author), Quax, P.H.A. (author), Gaubius instituut TNO (author)Proteases of the plasminogen activator (PA) and matrix metalloproteinase (MMP) system play an important role in smooth muscle cell (SMC) migration and neointima formation after vascular injury. Inhibition of either PAs or MMPs has previously been shown to result in decreased neointima formation in vivo. To inhibit both protease systems...article 2002
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van Baar, B.L.M. (author), Hulst, A.G. (author), Roberts, B. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)A method was developed for the liquid chromatographic-mass spectrometric (LC-MS) identification of extremely neurotoxic toxins. The method combines sample treatment in a safety containment and analysis of detoxified material in a common laboratory facility. The method was applied to the characterization of neat tetanus toxin and subsequent...article 2002
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Conesa, A. (author), Jeenes, D. (author), Archer, D.B. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author)Heme-containing peroxidases from white rot basidiomycetes, in contrast to most proteins of fungal origin, are poorly produced in industrial filamentous fungal strains. Factors limiting peroxidase production are believed to operate at the posttranslational level. In particular, insufficient availability of the prosthetic group which is required...article 2002
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Goedde, M.F. (author), Grimbergen, J.M. (author), Toet, K.H. (author), Sitter, T. (author), Quax, P.H. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)Background. The mesothelium has an important role in maintaining an adequate fibrinolytic capacity in the peritoneal cavity and thus in preventing the formation of fibrinous peritoneal adhesions by secreting the fibrinolytic enzyme tissue-type plasminogen activator (t-PA). The fibrinolytic activity of human mesothelial cells (HMCs) is...article 2001
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Oude Elferink, S.J.W.H. (author), Krooneman, J. (author), Gottschal, J.C. (author), Spoelstra, S.F. (author), Faber, F. (author), Driehuis, F. (author)The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without...article 2001
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Swift, R.J. (author), Karandikar, A. (author), Griffen, A.M. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Robson, G.D. (author), Trinci, A.P.J. (author), Wiebe, M.G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Aspergillus niger B1, a recombinant strain carrying 20 extra copies of the native glucoamylase gene, was grown in glucose-limited chemostat cultures supplemented with various organic nitrogen sources (dilution rate 0.12 ± 0.01 h-1, pH 5.4). In cultures supplemented with L-alanine, L-methionine, casamino acids, or peptone, specific glucoamylase ...article 2000
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Renwick, A.B. (author), Watts, P.S. (author), Edwards, R.J. (author), Barton, P.T. (author), Guyonnet, I. (author), Price, R.J. (author), Tredger, J.M. (author), Pelkonen, O. (author), Boobis, A.R. (author), Lake, B.G. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)The maintenance of the major hepatic cytochrome P450 (CYP) enzymes has been studied in precision-cut human liver slices cultured for up to 72 h in supplemented RPMI 1640 medium. The relative apoprotein levels of 11 CYP enzymes were determined using a panel of antipeptide antibodes. In addition, 7-ethoxyresorufin O-deethylase, tolbutamide...article 2000