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COST Action 'ImpARAS': What have we learnt to improve food allergy risk assessment. A summary of a 4 year networking consortium

Verhoeckx, K. (author), lindholm Bøgh, K. (author), Constable, A. (author), Epstein, M.M. (author), hoffmann Sommergruber, K. (author), Holzhauser, T. (author), Houben, G. (author), Kuehn, A. (author), Roggen, E. (author), O'Mahony, L. (author), Remington, B. (author), Crevel, R. (author)

The growing world population and increased pressure on agricultural resources are driving a shortage of dietary protein sources. As a result, industry is developing more sustainable novel food protein sources such as insects, algae and duckweed and using new processing techniques. Consumer exposure to these novel or processed proteins, could...

article 2020

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Risk of shared equipment in restaurants for consumers with peanut allergy: a simulation for preparing Asian foods: A simulation for preparing Asian foods

Remington, B.C. (author), Blom, W.M. (author), Bassa, B. (author), Koppelman, S.J. (author)

Allergic reactions to meals consumed outside the home are common and can be severe and sometimes fatal. Objective. To quantify the risk reduction potentially achieved by increasing an individual’s threshold sensitivity to peanut (such as by means of immunotherapy) in scenarios of peanut exposure through shared kitchen materials in a restaurant...

article 2020

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Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins

Remington, B. (author), Broekman, H.C.H. (author), Blom, W.M. (author), Capt, A. (author), Crevel, R.W.R. (author), Dimitrov, I. (author), Faeste, C.K. (author), Fernandez-Canton, R. (author), Giavi, S. (author), Houben, G.F. (author), Glenn, K.C. (author), Madsen, C.B. (author), Kruizinga, A.K. (author), Constable, A. (author)

The development and introduction of new dietary protein sources has the potential to improve food supply sustainability. Understanding the potential allergenicity of these new or modified proteins is crucial to ensure protection of public health. Exposure to new proteins may result in de novo sensitization, with or without clinical allergy, or...

article 2018

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Majority of shrimp-allergic patients are allergic to mealworm

Broekman, H. (author), Verhoeckx, K.C. (author), den Hartog Jager, C.F. (author), Kruizinga, A.G. (author), Pronk-Kleinjan, M. (author), Remington, B.C. (author), Bruijnzeel-Koomen, C.A. (author), Houben, G.F. (author), Knulst, A.C. (author)

The growing world population motivates the exploration of new sustainable protein sources to ensure food security. Insects such as mealworm (Tenebrio molitor) are promising candidates, with active ongoing marketing efforts within America and Europe. This warrants assessment of the potential risks. Toxicologic and microbiological risks were...

article 2016

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Allergenicity assessment strategy for novel food proteins and protein sources

Verhoeckx, K. (author), Broekman, H. (author), Knulst, A. (author), Houben, G. (author)

To solve the future food insecurity problem, alternative and sustainable protein sources (e.g. insects, rapeseed, fava bean and algae) are now being explored for the production of food and feed. To approve these novel protein sources for future food a comprehensive risk assessment is needed according to the European food legislation....

article 2016

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Clinical relevance of sensitization to lupine in peanut-sensitized adults

Peeters, K.A.B.M. (author), Koppelman, S.J. (author), Penninks, A.H. (author), Lebens, A. (author), Bruijnzeel-Koomen, C.A.F.M. (author), Hefle, S.L. (author), Taylor, S.L. (author), van Hoffen, E. (author), Knulst, A.C. (author), TNO Kwaliteit van Leven (author)

Background: The use of lupine in food has been increasing during the last decade and allergic reactions to lupine have been reported, especially in peanut-allergic patients. The frequency and the degree of cross-reactivity to other legumes are not known. The aim of the study was to investigate the frequency of sensitization to lupine, and in...

article 2009

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Lupine allergy: Not simply cross-reactivity with peanut or soy

Peeters, K.A.B.M. (author), Nordlee, J.A. (author), Penninks, A.H. (author), Chen, L. (author), Goodman, R.E. (author), Bruijnzeel-Koomen, C.A.F.M. (author), Hefle, S.L. (author), Taylor, S.L. (author), Knulst, A.C. (author), TNO Kwaliteit van Leven (author)

Background: Reports of lupine allergy are increasing as its use in food products increases. Lupine allergy might be the consequence of cross-reactivity after sensitization to peanut or other legumes or de novo sensitization. Lupine allergens have not been completely characterized. Objectives: We sought to identify allergens associated with...

article 2007

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Development of an enzyme-linked immunosorbent assay method to detect mustard protein in mustard seed oil

Koppelman, S.J. (author), Vlooswijk, R. (author), Bottger, G. (author), van Duijn, G. (author), van der Schaft, P. (author), Dekker, J. (author), van Bemgen, H. (author), TNO Kwaliteit van Leven (author)

An enzyme-linked immunosorbent assay for the detection of mustard protein was developed. The assay is based on a polyclonal antiserum directed against a mixture of mustard proteins raised in rabbits. The assay has a detection limit of 1.5 ppm (milligrams per kilogram) and is suitable for the detection of traces of mustard protein in mustard seed...

article 2007

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Detection of a soluble form of BACE-1 in human cerebrospinal fluid by a sensitive activity assay

TNO Kwaliteit van Leven (author), Verheijen, J.H. (author), Huisman, L.G.M. (author), van Lent, N. (author), Neumann, U. (author), Paganetti, P. (author), Erik Hack, C. (author), Bouwman, F. (author), Lindeman, J. (author), Bollen, E.L.E.M. (author), Hanemaaijer, R. (author)

Background: Formation of deposits of the insoluble amyloid β-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The β-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid formation is the cleavage of APP by...

article 2006

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Generation of a novel replication-incompetent adenoviral vector derived from human adenovirus type 49: Manufacture on PER.C6 cells, tropism and immunogenicity

TNO Kwaliteit van Leven (author), Lemckert, A.A.C. (author), Grimbergen, J. (author), Smits, S. (author), Hartkoorn, E. (author), Holterman, L. (author), Berkhout, B. (author), Barouch, D.H. (author), Vogels, R. (author), Quax, P. (author), Goudsmit, J. (author), Havenga, M.J.E. (author)

Recombinant adenoviral vectors based on type 5 (rAd5) show great promise as a vaccine carrier. However, neutralizing activity against Ad5 is prevalent and high-titred among human populations, and significantly dampens Ad5-based vaccine modalities. The generation of alternative adenoviral vectors with low seroprevalence thus receives much...

article 2006

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Purification and immunoglobulin E-binding properties of peanut allergen Ara h 6: Evidence for cross-reactivity with Ara h 2

Koppelman, S.J. (author), de Jong, G.A.H. (author), de Laaper-Ertmann, M. (author), Peeters, K.A.B.M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Knol, E.F. (author), TNO Kwaliteit van Leven (author)

Background: IgE-binding peanut proteins smaller than 15 kDa were previously identified as potential allergens in the majority of our peanut allergic population. Objective: To characterize the novel allergen in order to determine whether it was similar to one of the thus far identified recombinant peanut allergens (Ara h 1-7). Methods: An IgE...

article 2005

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Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy

Mittag, D. (author), Akkerdaas, J. (author), Ballmer-Weber, B.K. (author), Vogel, L. (author), Wensing, M. (author), Becker, W.M. (author), Koppelman, S.J. (author), Knulst, A.C. (author), Helbling, A. (author), Hefle, S.L. (author), van Ree, R. (author), Vieths, S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)

We recently described patients with soybean allergy mainly mediated by cross-reactivity to birch pollen allergens. A majority of those patients were reported to have peanut allergy. We sought to study the occurrence of peanut allergy in patients allergic to birch pollen and characterized the Bet v 1-homologous peanut allergen Ara h 8....

article 2004

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Patients with anaphylaxis to pea can have peanut allergy caused by cross-reactive lgE to vicilin (Ara h 1)

Wensing, M. (author), Knulst, A.C. (author), Piersma, S. (author), O'Kane, F. (author), Knol, E.F. (author), Koppelman, S.J. (author)

Background: Serologic cross-reactivity among legumes has been described; however, it is rarely clinically significant. In this study 3 patients with a history of anaphylaxis to pea are described who subsequently had symptoms after ingestion of peanut. Objective: We investigated whether the peanut-related symptoms were due to cross-reactivity...

article 2003

document

A neoantigenic determinant in the D-dimer fragment of fibrin

Lugovskoy, E.V. (author), Kolesnikova, I.N. (author), Gritsenko, P.G. (author), Zolotareva, E.N. (author), Gaffney, P. (author), Nieuwenhuizen, W. (author), Komisarenko, S.V. (author), TNO Preventie en Gezondheid (author)

Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III...

article 2002

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Purification and substrate specificity of transglutaminases from blood and Streptoverticillium mobaraense

de Jong, G.A.H. (author), Wijngaards, G. (author), Boumans, H. (author), Koppelman, S. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A procedure for a fast and simple purification of bovine plasma transglutaminase was developed, which resulted in a homogeneous enzyme preparation. Two different procedures were developed for the purification of pig erythrocyte transglutaminase, both of which resulted in partial purification. Both enzymes were used in cross-linking reactions of...

article 2001

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Comparison of different immunochemical methods for the detection and quantification of hazelnut proteins in food products

Koppelman, S. (author), Knulst, A.C. (author), Koers, W.J. (author), Penninks, A.H. (author), Peppelman, H. (author), Vlooswijk, R. (author), Pigmans, I. (author), van Duijn, G. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Hazelnuts are widely used in the food industry owing to their nutritive value and taste. The amount of hazelnut present in a recipe is usually considered as a mark of quality. On the other hand, contamination of foods that normally do not contain hazelnuts is a threat for patients with a hazelnut allergy. For this reason, the availability of a...

article 1999

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Immune responses and side effects of five different oil-based adjuvants in mice

Leenaars, M. (author), Koedam, M.A. (author), Hendriksen, C.F.M. (author), Claassen, E. (author), TNO Preventie en Gezondheid (author)

In this study, five different oil based adjuvants were compared to assess efficacy and side effects. Mice were injected subcutaneously (s.c.) or intraperitoneally (i.p.) with a weak immunogen (synthetic peptide) emulsified in Freund's adjuvant (FA), Specol, RIBI, TiterMax or Montanide ISA50. Efficacy of adjuvants was evaluated based on their...

article 1998

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Thrombus detection using a Tc-99m labelled antifibrin monoclonal antibody (MoAb) experiments in vitro and in animals

Gaubius Instituut TNO (author), Wasser, M.N.J.M. (author), Pauwels, E.K.J. (author), Nieuwenhuizen, W. (author)

This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment by the antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in...

article 1990

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A monoclonal antibody, specific for human fibrinogen, fibrinopeptide A-containing fragments and not reacting with free fibrinopeptide A

Koppert, P.W. (author), Huijsmans, C.M.G. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)

Spleen cells of BALB/c mice, immunized with fragments Y of normal human fibrinogen, were fused with P3 x 63 Ag 8653 myeloma cells. A clone was found which produces monoclonal antibodies (Mab-Y18) of the IgM?? type. Mab-Y18 is immunoreactive with normal human fibrinogen, and its fragments X, Y, N-terminal disulphide knot, A??-chain, and A??...

article 1985

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Alloreactive cytotoxic T lymphocytes from aged mice express increased lysis of autologous and third-party target cells

Kruisbeek, A.M. (author), Steinmeier, F.A. (author), Instituut voor experimentele gerontologie TNO (author)

Much data support the notion that with increasing age a decline in T cell effector function occurs. In the present study, qualitative rather than quantitative age-related changes in vitro alloreactive cytotoxic T lymphocyte (CTL) responses were observed. The level of specific alloreactive CTL responses was not consistently lower in aged mice,...

article 1980