Reproducibility of food challenge to cow's milk: Systematic review with individual participant data meta-analysis

Background: Cow's milk (CM) is an increasingly common cause of severe allergic reactions, but there is uncertainty with respect to severity of reactions at low-level CM exposure, as well as the reproducibility of reaction thresholds. Objective: We undertook an individual participant data (IPD) meta-analysis of studies reporting double-blind,...

A multi-center assessment to compare residual allergenicity of partial hydrolyzed whey proteins in a murine model for cow’s milk allergy – Comparison to the single parameter guinea pig model

Introduction This 4-center study is part of a project to validate a food allergy murine model for safety testing of hydrolyzed infant formulas. Aim The aim of the current multi-center experiment was to evaluate the residual allergenicity of three partial hydrolyzed whey proteins (pWH) in a multiple-parameter cow’s milk allergy murine model and...

Near real-time large scale (sensor) data provisioning for PLF

Think big, start small. With that thought in mind, Smart Dairy Farming (SDF) developed a platform to make real-time sensor data from different farms available, for model developers to support dairy farmers in Precision Livestock Farming. The data has been made available via a standard interface on an open platform in real-time at the individual...

Allergen reference doses for precautionary labeling (VITAL 2.0): Clinical implications

Background There has been a dramatic proliferation of precautionary labeling by manufacturers to mitigate the perceived risk from low-level contamination from allergens in food. This has resulted in a significant reduction in choice of potentially safe foods for allergic consumers. Objectives We aimed to establish reference doses for 11 commonly...

Interlaboratory evaluation of a cow's milk allergy mouse model to assess the allergenicity of hydrolysed cow's milk based infant formulas

This study describes two phases of a multi-phase project aiming to validate a mouse model for cow's milk allergy to assess the potential allergenicity of hydrolysed cow's milk based infant formulas (claim support EC-directive 2006/141/E). The transferability and the discriminatory power of this model was evaluated in 4 research centers. Mice...

Double-blind placebo-controlled food challenges in children with alleged cow's milk allergy: Prevention of unnecessary elimination diets and determination of eliciting doses

Background: Children with cow's milk allergy (CMA) need a cow's milk protein (CMP) free diet to prevent allergic reactions. For this, reliable allergy-information on the label of food products is essential to avoid products containing the allergen. On the other hand, both overzealous labeling and misdiagnosis that result in unnecessary...

Safety evaluation of an IPP tripeptide-containing milk protein hydrolysate

Tensguard™ is a milk protein hydrolysate containing the lactotripeptide IPP. It is derived from cow's milk, which is present in the human diet and has a safe history of consumption. The final Tensguard™ product, a supplement or a functional food ingredient, is intended for use by people who want to follow a healthy diet and lifestyle in order to...

Dieting in children: A population-based study in children aged between 9 and 12 years

Biochemical and functional modulation of the cartilage collagen network by IGF1, TGFβ2 and FGF2

Objective: Examine effects of insulin-like growth factor 1 (IGF1), transforming growth factor β2 (TGFβ2) and fibroblast growth factor 2 (FGF2) on proteoglycan and collagen network and biomechanical properties of the newly formed cartilage matrix. Methods: Bovine articular chondrocytes were cultured in alginate beads for 3 weeks with or without...

Changes in Water Content, Collagen Degradation, Collagen Content, and Concentration in Repeated Biopsies of the Cervix of Pregnant Cows

The objective of the present study was to assess if cervical ripeness could be quantified by measuring the percentage of denaturation of the collagen network of the stromal layer. Biopsy specimens from the caudal part of the cervix were obtained from nine pluriparous cows between Days 149 and 157 of gestation (second-trimester biopsy), at...

Influence of feeding various phosphorus sources on apparent digestibility of phosphorus in dairy cows

The present investigated differences in the apparent digestibility of phosphorus (ADP) in lactating cows fed various feed materials containing organic forms of phosphorus (P) or a highly available inorganic phosphorus source. Cows milking an average of 36 kg energy corrected milk (ECM) were used in a 4 x 4 Latin square design with 4-week periods...

Comparison of antibody responses to hen's egg and cow's milk proteins in orally sensitized rats and food-allergic patients

Background: No adequate enteral sensitization models are available to study food allergy and the allergenicity of food proteins. To further validate an enteral brown Norway (BN) rat sensitization model under development, we studied specific protein recognition to determine whether a comparable pattern of proteins is recognized by the rat immune...

A novel pyrroleninone cross-link from bovine dentine

The aim was to identify suspect collagen cross-links in dentine, eluting close to known cross-links in ion-exchange HPLC. Bovine tooth roots as source of dentine were powdered, demineralised, reduced, and acid-hydrolysed. Cross-linking amino acids were isolated from the acid hydrolysate by size exclusion, adsorption, and sequential ion exchange...