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Tonk, E.C.M. (author), de Groot, D.M.G. (author), Wolterbeek, A.P.M. (author), Penninks, A.H. (author), Waalkens-Berendsen, I.D.H. (author), Piersma, A.H. (author), van Loveren, H. (author)The susceptibility of developing immune system to chemical disruption warrants the assessment of immune parameters in reproductive and developmental testing protocols. In this study, a wide range of immune endpoints was included in an extended one-generation reproduction toxicity study (EOGRTS) design to determine the relative sensitivity of...article 2013
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de Jong, W.H. (author), Arts, J.H.E. (author), de Klerk, A. (author), Schijf, M.A. (author), Ezendam, J. (author), Kuper, C.F. (author), van Loveren, H. (author), TNO Kwaliteit van Leven (author)There are currently no validated animal models that can identify low molecular weight (LMW) respiratory sensitizers. The Local Lymph Node Assay (LLNA) is a validated animal model developed to detect contact sensitizers using skin exposure, but all LMW respiratory sensitizers tested so far were also positive in this assay. Discrimination between...article 2009
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), van den Bergh, P. (author), Dobber, R. (author), Ramlal, S. (author), Rozing, J. (author), Nagelkerken, L. (author)The presence of the opioid peptides α- and β-endorphin (-End) but not methionine enkephalin (Met-enk) in in vitro cultures of purified CD4+ T cells, stimulated with concanavalin A in the presence of irradiated spleen cells, resulted in a threefold stimulation of IL-2, IL-4, and IFN-γ production. The stimulating effect was dependent on the...article 1994
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), van den Bergh, P. (author), Rozing, J. (author), Nagelkerken, L. (author)In the present study we show that the opioid peptide β-endorphin (β-End) enhances Ia expression on murine B cells in cultures of unseparated spleen cells, mediated by low concentrations of IL4 in the absence of antigenic or mitogenic stimulation. Since this effect was not found with purified B cells and no enhancement of IL-4 receptor expression...article 1993
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Kruisbeek, A.M. (author), Instituut voor experimentele gerontologie TNO (author)In the present review the results obtained so far with thymic epithelial culture supernatants (TES) or cocultivation of potential precursor T cells with TE cells will be discussed and compared with the effects reported for other thymic factors in studies on T cell differentiation in vitro. These studies can be divided into those involved with T...article 1979
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Vos, O. (author), Wilschut, I.J.C. (author), Medisch Biologisch Laboratorium TNO (author)Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after...article 1979
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Instituut voor experimentele gerontologie TNO (author), Kruisbeek, A.M. (author)Age related changes in lipopolysaccharide (LPS) and concanavalin A (ConA) responsiveness of rat spleen lymphocytes as judged by 14C TdR incorporation were studied. It was found that responses to both mitogens decreased with advancing age. This report shows that the reduced 14C TdR incorporation could not be attributed to decreased cell survival...article 1976
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Radiobiologisch Instituut TNO (author), van den Berg, K.J. (author), Betel, I. (author)Chemicals/CAS: 2 amino 2 methylpropionic acid, 62-57-7; amino acid, 65072-01-7; concanavalin A, 11028-71-0; sodium, 7440-23-5; Amino Acids; Aminoisobutyric Acids; Concanavalin A, 11028-71-0; Cyclopentanes; Sodium, 7440-23-5article 1973