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Verdoes, J.C. (author), Punt, P.J. (author), Burlingame, R. (author), Bartels, J. (author), van Dijk, R. (author), Slump, E. (author), Meens, M. (author), Joosten, R. (author), Emalfarb, M. (author), TNO Kwaliteit van Leven (author)A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chiysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/ug ofplasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector...article 2007
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Gusakov, A.V. (author), Sinitsyn, A.P. (author), Salanovich, T.N. (author), Bukhtojarov, F.E. (author), Markov, A.V. (author), Ustinov, B.B. (author), Zeijl, C.V. (author), Punt, P. (author), Burlingame, R. (author), TNO Kwaliteit van Leven (author)Two forms of cellobiohydrolase I (CBH I, Cel7A) were purified from the culture ultrafiltrate of a mutant strain of the fungus Chrysosporium lucknowense, an industrial producer of cellulases and hemicellulases. The enzymes had different molecular masses (52 and 65 kDa, SDS-PAGE data) but the same pI (4.5). Peptide sequencing showed that a single...article 2005