Ileal digestibility and urinary excretion of D-xylose and L-arabinose in ileostomized adult roosters.

An experiment with ileostomized adult roosters was conducted to determine the ileal digestibility and urinary excretion of D-xylose and L-arabinose. As a reference, D-glucose was included in the experiment. The sugars were tested at graded dietary levels of 2.5, 5.0, 7.5, and 10.0%. Mean ileal digestibility of D-glucose and D-xylose was nearly...

Nutritional implications and metabolizable energy value of D-xylose and L-arabinose in chicks.

An experiment was conducted to examine the effects of graded levels (2.5, 5.0, 7.5, 10.0, and 15.0%) of dietary D-xylose or L-arabinose on chick performance. As reference, D-glucose was included in the experiment. A second experiment was performed to determine the AMEn of D-xylose and L-arabinose. Results of Experiment 1 showed a significant...

Effect of variable protein contents in diets containing Phaseolus vulgaris beans on performance, organ weights and blood variables in piglets, rats and chickens

A comparison was made of the effects of antinutritional factors present in Phaseolus vulgaris on piglets, rats and chickens. Also the hypothesis of whether the negative effect on weight gain due to the inclusion of raw Phaseolus vulgaris in the diet can be attributed to an insufficient supply of amino acids was tested. Test diets containing 200...

Reduction of polyneuronal innervation of muscle cells in tissue culture after long-term indirect stimulation

In combined cultures of chick muscle cells and ciliary ganglia, the effect of indirect, long-term stimulation on the number of neuromuscular contacts on individual muscle cells was studied. Seven days of phasic stimulation resulted in a marked reduction in the number of neuromuscular contacts, the majority of the muscle cells becoming innervated...

Competition between neuromuscular contacts in tissue culture, induced by stimulation

Chemicals/CAS: tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7

Effects of extracts of denervated muscles on the morphology of cultured muscle cells

Previously tropic effects of extracts from whole chick embryos and from innervated muscles on cultured muscle cells were described. The present study demonstrated similar effects of extracts from 10-days denervated chick muscles. Extracts from innervated as well as from denervated muscles exsanguinated in vivo with saline prior to dissection...

DNA repair and survival in UV-irradiated chicken-embryo fibroblasts

The role of the pyrimidine dimer in cell killing, DNA synthesis and repair has been studied by utilizing the light-requiring DNA-repair mechanism of photo-reactivation in UV-irradiated chicken-embryo fibroblasts. Survival, as measured by colony-forming ability at 41??C, is increased in cells left in the light. The initial inhibition of DNA...

Sensitivity of several cell systems to acrylamide

Chick spinal ganglia, chick muscle cells combined with mouse spinal cord explants, C1300 neuroblastoma cells, Chinese hamster ovary cells and newborn rat cerebral cells were exposed to various concentrations of acrylamide in culture. Four morphological and 1 electrophysiological parameter were applied in order to score toxic effects. It appeared...

Effects of innervation by ciliary ganglia on developing muscle in vitro

Electrophysiological and pharmacological properties of neuromuscular junctions formed in tissue culture between chick ciliary ganglia and chick skeletal muscle cells have been studied. Functional neuromuscular junctions are formed already within 24h. No functional acetylcholine (ACh) esterase is present at these endplates. The neurites conduct...

Trophic support by neural explants of cultured muscle fibers

The influence of various neural explants on the morphology and the survival of chick muscle fibers was studied. A method was developed to evaluate the condition of the muscle fibers using the following four morphological parameters: cross striation, thickness, number of fibers and absence of vacuoles. Chick as well as mouse spinal cord explants...

Target recognition and synapse formation by ciliary-ganglion neurons in tissue culture

A less complicated source of neurons suitable for this type of studies is the parasympathetic ciliary ganglion. In the pigeon and in the chick this ganglion is known to contain only two classes of neurons, both of which are cholinoceptive and cholinergic and that innervate the muscle fibres of the choroid plexus of the eye, the sphincter iridis...