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Nelson, G. (author), Kozlova-Zwinderman, O. (author), Collis, A.J. (author), Knight, M.R. (author), Fincham, J.R.S. (author), Stanger, C.P. (author), Renwick, A. (author), Hessing, J.G.M. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Read, N.D. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and...article 2004
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Jaworska, J. (author), van Genderen-Takken, H. (author), Hanstveit, A. (author), van de Plassche, E. (author), Feijtel, T. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)In the long-term cooperative project Voluntary Plan of Action (1990) between the Dutch Soap and Detergent Association (NVZ) and the Dutch Ministry of Housing, Spatial Planning and the Environment (VROM) environmental risk assessments of several main components of laundry cleaning formulations were completed. As a part of that project the...article 2002
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Schokker, E.P. (author), Dalgleish, D.G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Calcium-induced flocculation of caseinate-stabilized soybean oil-in- water emulsions in conditions of Couette flow was studied. A concentrated emulsion (20% oil, 0.5-2.0% sodium caseinate in 20 mM imidazole, pH 7) was diluted 20 times in buffer containing concentrations of CaCl2 between 9 and 17 mM and sheared at rates between 335 and 1340 s-1....article 2000
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Anderson, D. (author), Yardley-Jones, A. (author), Hambly, R.J. (author), Vives-Bauza, C. (author), Smykatz-Kloss, V. (author), Chua-anusorn, W. (author), Webb, J. (author), TNO BIBRA International (author)Thalassaemia is a group of genetic diseases where haemoglobin synthesis is impaired. This chronic anaemia leads to increased dietary iron absorption, which develops into iron overload pathology. Treatment through regular transfusions increases oxygen capacity but also provides iron through the red cells' haemoglobin. An essential treatment, in...article 2000
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Fidder, A. (author), Noort, D. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)The development of a procedure for retrospective detection and quantitation of exposure to the arsenical dichloro(2-chlorovinyl)arsine (lewisite; L1) has been initiated. Upon incubation of human blood with [14C]L1 (20 nM-0.2 mM) in vitro, more than 90% of the total radioactivity was found in the erythrocytes and 25-50% of the radioactivity...article 2000
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Schowanek, D. (author), McAvoy, D. (author), Versteeg, D. (author), Hanstveit, A. (author), TNO Voeding (author)This study tests the hypothesis that the apparent toxicity of strong chelators in standard algal growth inhibition tests (e.g. method OECD 201, EC C.3., ISO 8692) is related to essential trace metal bioavailability. This hypothesis was investigated for the chelator [S,S]-ethylene diamine disuccinate ([S,S]-EDDS) and the green alga Chlorella...article 1996