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van der Bom, J.G. (author), de Maat, M.P.M. (author), Bots, M.L. (author), Haverkate, F. (author), de Jong, P.T.V.M. (author), Hofman, A. (author), Kluft, C. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)An association between increased plasma fibrinogen and an increased risk for myocardial infarction (MI) is well established, but the nature of this association is subject to debate. Our aim was to shed light on the potentially causal nature of this association. We examined whether increased plasma fibrinogen, due to a condition that is...article 1998
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- van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author) article 1997
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Arts, J. (author), Herr, I. (author), Lansink, M. (author), Angel, P. (author), Kooistra, T. (author), TNO Preventie en Gezondheid (author)Tissue-type plasminogen activator (t-PA) gene expression in human endothelial cells and HeLa cells is stimulated by the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) at the level of transcription. To study the mechanism of transcriptional regulation, we have characterized a segment of the t-PA gene extending from -135 to +100...article 1997
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- Dell' Acqua, G. (author), Iacoviello, L. (author), D' Orazio, A. (author), Di Bitondo, R. (author), Di Castelnuovo, A. (author), Donati, M.B. (author), Gaubius Instituut TNO (author) article 1997
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TNO Preventie en Gezondheid (author), van Arkel, C. (author), Hopstaken, C.M. (author), Zurcher, C. (author), Bos, N.A. (author), Kroese, F.G.M. (author), Savelkoul, H.F.J. (author), Benner, R. (author), Radl, J. (author)Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor cells of MG are unknown, but...article 1997
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Mientjes, E.J. (author), Hochleitner, K. (author), Luiten-Schuite, A. (author), van Delft, J.H.M. (author), Thomale, J. (author), Berends, F. (author), Rajewsky, M.F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)LacZ transgenic mice are suitable for short-term mutagenicity studies in vivo. Mutagenicity in these mice is determined in the lacZ transgene. Since the lacZ gene is of bacterial origin the question has been raised whether DNA-adduct formation and repair in the transgene are comparable to those in total genomic DNA. Mice were treated with N...article 1996
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van der Vossen, J.M.B.M. (author), Hofstra, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The rapid identification of spoilage microorganisms is of eminent importance to the food industry. It provides the food industry with the opportunity to reduce economical losses by designing adequate intervention measures. The use of identification systems based on biochemical and physiological characteristics resulted often in disappointing...article 1996
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Boot, H.J. (author), Kolen, C.P.A.M. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)The most-dominant surface-exposed protein in many bacterial species is the S-protein. This protein crystallises into a regular monolayer on the outside surface of the bacteria: the S-layer. Lactobacillus acidophilus harbours two S-protein-encoding genes, slpA and slpB, only one of which (slpA) is expressed. In this study, we show by polymerase...article 1996
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van Setten, P.A. (author), Monnens, L.A.H. (author), Verstraten, R.G.G. (author), van den Heuvel, L.P.W.J. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)The epidemic form of the hemolytic uremic syndrome (HUS) has been associated with a verocytotoxin producing Escherichia coli infection. Endothelial cell damage of glomeruli and arterioles of the kidney plays a central role in the pathogenesis of HUS. A number of observations in vivo and in vitro indicate that inflammatory mediators contribute to...article 1996
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Visser, C.J.T. (author), Bruggink, A.H. (author), Korc, M. (author), Kobrin, M.S. (author), de Weger, R.A. (author), Seifert-Bock, I. (author), van Blokland, W.T.M. (author), van Garderen Hoetmer, A. (author), Woutersen, R.A. (author), TNO Voeding (author)Using immunohistochemistry, Northern blotting and a semi-quantitative PCR technique, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and epidermal growth factor receptor (EGFR) expression were studied in the pancreas of N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. After initiation pancreatic carcinogenesis was...article 1996
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Offermans, M.T.C. (author), Struyk, L. (author), de Geus, B. (author), Breedveld, F.C. (author), van den Elsen, P.J. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)In order to define the extent of T cell heterogeneity and clonality, unique DNA sequences in the junctional region in rearranged T cell receptor (TcR) genes can be studied. For this purpose we have adapted a non-denaturing nucleic acid gel electrophoresis procedure to detect TcR junctional diversity. Detection of junctional diversity is based...article 1996
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Slats, P.A. (author), Bhola, B. (author), Evers, J.J.M. (author), Dijkhuizen, G. (author), TNO Fysisch en Elektronisch Laboratorium (author)Logistic chain modelling is very important in improving the overall performance of the total logistic chain. Logistic models provide support for a large range of applications, such as analysing bottlenecks, improving customer service, configuring new logistic chains and adapting existing chains to new products and markets. Modelling the logistic...article 1995
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Offermans, M.T.C. (author), Sonneveld, R.D. (author), Bakker, E. (author), Deutz-Terlouw, P.P. (author), de Geus, B. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)Two nucleic acid gel electrophoresis techniques were tested as a possible tool for analyzing junctional diversity in rearranged T cell receptor (TcR) sequences in order to define the extent of T cell heterogeneity. For this purpose denaturing gradient gel electrophoresis (DGGE) as well as non-denaturing gel electrophoresis (nDGE) techniques have...article 1995
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van Mechelen, J.R. (author), Smits, M. (author), Douma, A.C. (author), Rouster, J. (author), Cameron-Mills, V. (author), Heidekamp, F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its...article 1995
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Le Jeune, C. (author), Lonvaud-Funel, A. (author), ten Brink, B. (author), Hofstra, H. (author), van der Vossen, J.M.B.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All...article 1995
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Baleiras Couto, M.M. (author), Vogels, J.T.W.E. (author), Hofstra, H. (author), Huis in 'tVeld, J.H.J. (author), van der Vossen, J.M.B.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The random amplified polymorphic DNA (RAPD) assay and the restriction enzyme analysis of PCR amplified rDNA are compared for the identification of the common spoilage yeasts Zygosaccharomyces bailii, Z. rouxii, Saccharomyces cerevisiae, Candida valida and C. lipolytica. Both techniques proved to be adequate tools for yeast identification. Since...article 1995
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Otter, M. (author), Kuiper, J. (author), Rijken, D. (author), van Zonneveld, A.-J. (author), TNO Preventie en Gezondheid (author)To characterize the primary structure of rat Vn, a cDNA library constructed from freshly isolated rat hepatocytes, was screened with a human Vn cDNA probe. Comparison of the sequence of the obtained rat cDNA clone with the sequences of human, mouse and rabbit Vn cDNA's showed predominantly consensus in the somatomedin B domain, the RGD-sequence...article 1995
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van den Brink, J.M. (author), van Zeijl, C.M.J. (author), Brons, J.F. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In this paper, we describe the cloning and molecular characterization of the Aspergillus niger cytochrome P450 reductase (CPR) gene, cprA. Attempts to clone the cprA gene by heterologous hybridization techniques were unsuccessful. Using the polymerase chain reaction (PCR) with degenerate primers based on conserved regions found in cpr genes from...article 1995
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Primatencentrum TNO (author), Slierendregt, B.L. (author), Otting, N. (author), Kenter, M. (author), Bontrop, R.E. (author)Allelic diversity at the major histocompatibility complex class II DP locus of rhesus macaques was studied by sequencing exon 2 of Mamu-DPA1 and -DPB1 genes. The Mamu-DPA1 gene is apparently invariant, whereas the Mamu-DPB1 locus displays polymorphism. Here we report the characterization of 1 Mamu-DPA1 and 13 Mamu-DPB1 alleles which were...article 1995
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Primatencentrum TNO (author), Otting, N. (author), Bontrop, R.E. (author)The evolutionary relationships among Mhc-DPA1 alleles of various nonhuman primate species was studied by sequence analysis of exon 2. Here we report the nucleotide sequences of 15 Mhc-DPA1 alleles obtained from several great ape and Old and New World monkey species. Comparison with their human homologues reveals that alleles can be grouped into...article 1995