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Hollestelle, S.C.G. (author), de Vries, M.R. (author), van Keulen, J.K. (author), Schoneveld, A.H. (author), Vink, A. (author), Strijder, C.F. (author), van Middelaar, B.J. (author), Pasterkamp, G. (author), Q́uax, P.H.A. (author), de Kleijn, D.P.V. (author), TNO Preventie en Gezondheid (author)Background-Toll-like receptor 4 (Tlr4) is the receptor for exogenous lipopolysaccharides (LPS). Expression of endogenous Tlr4 ligands, heat shock protein 60 (Hsp60) and extra domain A of fibronectin, has been observed in arthritic and oncological specimens in which matrix turnover is an important feature. In atherosclerosis, outward remodeling...article 2004
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Klaasse, E. (author), de Ligt, R.A.F. (author), Roerink, S.F. (author), Lorenzen, A. (author), Milligan, G. (author), Leurs, R. (author), IJzerman, A.P. (author), TNO Voeding (author)We studied fusion proteins between the human adenosine A1 receptor and different 351Cys-mutated Gi1 α-subunits (A1-Giα) with respect to two important concepts in receptor pharmacology, i.e. allosteric modulation and constitutive activity/inverse agonism. The aim of our study was twofold. We first analysed whether such fusion products are still...article 2004
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Wijnands, M.V.W. (author), van Erk, M.J. (author), Doornbos, R.P. (author), Krul, C.A.M. (author), Woutersen, R.A. (author)The effects of different dietary compounds on the formation of aberrant crypt foci (ACF) and colorectal tumours and on the expression of a selection of genes were studied in rats. Azoxymethane-treated male F344 rats were fed either a control diet or a diet containing 10% wheat bran (WB), 0.2% curcumin (CUR), 4% rutin (RUT) or 0.04% benzyl...article 2004
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van Nuenen, M.H.M.C. (author), Venema, K. (author), van der Woude, J.C.J. (author), Kuipers, E.J. (author), TNO Voeding (author)The hypothesis was studied that intestinal microbial metabolites play a role in the pathogenesis of inflammatory bowel disease. For that purpose, an in vitro model of the colon was inoculated with fresh feces of six healthy individuals and eight inflammatory bowel disease patients. Samples were taken from the model over time to analyze...article 2004
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O'Kane, F.E. (author), Happe, R.P. (author), Vereijken, J.M. (author), Gruppen, H. (author), van Boekel, M.A.J.S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)The gelling characteristics of two vicilin fractions from pea (Pisum sativum L.) were compared over a range of pH and salt conditions after preliminary results showed that despite having equal opportunity to unfold, and expose hydrophobic residues, they had different minimum gelling concentrations (at pH 7.6). Furthermore, at this pH one...article 2004
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de Ridder, H.A.J. (author), Vrijhoef, R. (author)The building and construction industry has a large contribution and impact on society, e.g. economical and environmental, involving a vast spectrum of stakeholders. However, the value delivering performance of the industry has often been criticized. The predictability of the value, price and costs of built objects has proven to be difficult,...conference paper 2004
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TNO Preventie en Gezondheid (author), Mandard, S. (author), Zandbergen, F. (author), Nguan, S.T. (author), Escher, P. (author), Patsouris, D. (author), Koenig, W. (author), Kleemann, R. (author), Bakker, A. (author), Veenman, F. (author), Wahli, W. (author), Müller, M. (author), Kersten, S. (author)The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was previously identified as a novel adipocytokine that was up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. To further characterize FIAF, we studied regulation of FIAF mRNA and protein in liver and adipose cell lines as well as in...article 2004
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TNO Preventie en Gezondheid (author), Ha-Vinh, R. (author), Alanay, Y. (author), Bank, R.A. (author), Campos-Xavier, A.B. (author), Zankl, A. (author), Superti-Furga, A. (author), Bonafé, L. (author)Bruck syndrome (BS) is a recessively-inherited phenotypic disorder featuring the unusual combination of skeletal changes resembling osteogenesis imperfects (OI) with congenital contractures of the large joints. Clinical heterogeneity is apparent in cases reported thus far. While the genes coding for collagen 1 chains are unaffected in BS, there...article 2004
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TNO Zeist (author), van den Broek, L.A.M. (author), Pouvreau, L. (author), Lommerse, G. (author), Schipper, B. (author), van Koningsveld, G.A. (author), Gruppen, H. (author)In the present study the structural properties of potato protease inhibitor 1 (PI-1) were studied as a function of temperature to elucidate its precipitation mechanism upon heating. A cDNA coding for PI-1 from cv. Bintje was cloned and expressed in Pichia pastoris. Using the recombinant PI-1 it was suggested that PI-1 behaves as a hexameric...article 2004
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Joosten, V. (author), Lokman, C. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Voeding (author)In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of...article 2003
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Martin, A.M. (author), Hammond, E. (author), Nolan, D. (author), Pace, C. (author), Den Boer, M. (author), Taylor, L. (author), Moore, H. (author), Martinez, O.P. (author), Christiansen, F.T. (author), Mallal, S. (author), TNO Preventie en Gezondheid (author)Nucleoside reverse-transcriptase inhibitor (NRTI) therapy for human immunodeficiency virus (HIV) infection has been associated with mitochondrial DNA (mtDNA) polymerase-γ inhibition and subsequent mtDNA depletion. Effects on mtDNA mutation, although suggested by critical involvement of polymerase-γ in DNA-repair reactions, are unknown. In the...article 2003
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Kralj, S. (author), Geel van - Schutten, G.H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus. Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in α-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known...article 2003
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Beauchamp, N.J. (author), van Achterberg, T.A.E. (author), Engelse, M.A. (author), Pannekoek, H. (author), de Vries, C.J.M. (author), Gaubius Instituut TNO (author)Migration and proliferation of vascular smooth muscle cells (SMCs) are key events in atherosclerosis. However, little is known about alterations in gene expression upon transition of the quiescent, contractile SMC to the proliferative SMC. We performed serial analysis of gene expression (SAGE) of cultured, human SMCs, either grown under resting...article 2003
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Brink, M. (author), de Goeij, A.F.P.M. (author), Weijenberg, M.P. (author), Roemen, G.M.J.M. (author), Lentjes, M.H.F.M. (author), Pachen, M.M.M. (author), Smits, K.M. (author), de Bruïne, A.P. (author), Goldbohm, R.A. (author), van den Brandt, P.A. (author)Activation of K-ras oncogene has been implicated in colorectal carcinogenesis, being mutated in 30-60% of the adenocarcinomas. In this study, 737 incident colorectal cancer (CRC) patients, originating from 120 852 men and women (55-69 years at baseline) participating in the Netherlands Cohort Study (NLCS), were studied in order to evaluate...article 2003
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Venema, K. (author), Maathuis, A.J.H. (author), TNO Voeding (author)A polymerase chain reaction (PCR)-based method was developed for the identification of isolates of Bifidobacterium at the species level. Using two Bifidobacterium-specific primers directed against the 16S ribosomal gene (Bif164 and Bif662), a PCR product was obtained from the type strains of 12 different bifidobacterial species that have been...article 2003
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Kievit, O. (author), Busker, R.W. (author), Kientz, C.E. (author), Marijnissen, J.C.M. (author)This paper describes the latest achievements in the development of a new rapid detector for biological warfare agents (BWA). The system uses aerodynamic particle sizing and matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry (MALDI ATOFMS) to analyse individual aerosol particles sampled directly from the...article 2003
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TNO BIBRA (author), Edwards, C.A. (author), Rumney, C. (author), Davies, M. (author), Parrett, A.M. (author), Dore, J. (author), Martin, F. (author), Schmitt, J. (author), Stahl, B. (author), Norin, E. (author), Midtvedt, T. (author), Rowland, I.R. (author), Heavey, P. (author), Köhler, H. (author), Stocks, B. (author), Schroten, H. (author)Objectives: Bacterial colonization of the infant gut may have important influences on the development of gastrointestinal, respiratory, and allergic disease. Early diet is a major determinant of the gut microflora. It is very difficult to carry out studies in human infants that can investigate the interaction of diet, flora, and mucosa. In this...article 2003
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TNO Voeding (author), Venema, K. (author), van Nuenen, M.H.M.C. (author), van den Heuvel, E.G. (author), Pool, W. (author), van der Vossen, J.M.B.M. (author)The objective of this study was to compare the in vivo effect of lactulose on faecal parameters with the effect in a dynamic, computer-controlled in vitro model of the proximal large intestine (TIM-2). Faecal samples from 10 human volunteers collected before (non-adapted) and after 1 week of treatment (10 g/day) with lactulose (lactulose-adapted...article 2003
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TNO Preventie en Gezondheid (author), van der Slot, A.J. (author), Zuurmond, A.M. (author), Bardoel, A.F.J. (author), Wijmenga, C. (author), Pruijs, H.E.H. (author), Sillence, D.O. (author), Brinckmann, J. (author), Abraham, D.J. (author), Black, C.M. (author), Verzijl, N. (author), de Groot, J. (author), Hanemaaijer, R. (author), te Koppele, J.M. (author), Huizinga, T.W.J. (author), Bank, R.A. (author)The hallmark of fibrotic processes is an excessive accumulation of collagen. The deposited collagen shows an increase in pyridinoline cross-links, which are derived from hydroxylated lysine residues within the telopeptides. This change in cross-linking is related to irreversible accumulation of collagen in fibrotic tissues. The increase in...article 2003
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Prins Maurits Laboratorium TNO (author), Broekhuijsen, M.P. (author), Larsson, P. (author), Johansson, A. (author), Byström, M. (author), Eriksson, U. (author), Larsson, E. (author), Prior, R.G. (author), Sjöstedt, A. (author), Titball, R.W. (author), Forsman, M. (author)Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and...article 2003