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Wang, C. (author), Chou, Y.K. (author), Rich, C.M. (author), Link, J.M. (author), Afentoulis, M.E. (author), van Noort, J.M. (author), Wawrousek, E.F. (author), Offner, H. (author), van den Bark, A.A. (author), TNO Kwaliteit van Leven (author)Alpha B-crystallin (αB) is a small heat shock protein that is strongly up-regulated in multiple sclerosis (MS) brain tissue, and can induce strong T cell responses. Assessing a potential encephalitogenic function for αB protein in MS and experimental autoimmune encephalomyelitis (EAE) has been challenging due to its ubiquitous expression that...article 2006
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van den Akker-van Marle, M.E. (author), Gurwitz, D. (author), Detmar, S.B. (author), Enzing, C.M. (author), Hopkins, M.M. (author), Gutierrez De Mesa, E. (author), Ibarreta, D. (author), TNO Kwaliteit van Leven (author)Only a few studies have addressed the cost-effectiveness of pharmacogenetics interventions in healthcare. Lack of health economics data on aspects of pharmacogenetics is perceived as one of the barriers hindering its implementation for improving drug safety. Thus, a recent Institute for Prospective Technological Studies (IPTS) study, entitled ...article 2006
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Schaafsma, G. (author), TNO Kwaliteit van Leven (author)Loss of muscle mass as a consequence of changes in protein metabolism during periods of catabolic stress is a serious complication in a variety of conditions. These conditions are weight loss programs, sarcopenia in the elderly and several clinical states. It appears from many studies that improved protein nutrition by ingestion of high-quality...article 2006
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TNO Kwaliteit van Leven (author), Uitterlinden, E.J. (author), Jahr, H. (author), Koevoet, J.L.M. (author), Jenniskens, Y.M. (author), Bierma-Zeinstra, S.M.A. (author), de Groot, J. (author), Verhaar, J.A.N. (author), Weinans, H. (author), van Osch, G.J.V.M. (author)Objective: To investigate the effect of glucosamine (GlcN) in a human osteoarthritic explant model on expression of genes involved in anabolic and catabolic activities of chondrocytes. Methods: Human osteoarthritic explants, obtained during knee arthroplasty surgery, were pre-cultured (3 days) and treated with glucosamine-hydrochloride (GlcN-HCl...article 2006
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Delsing, D.J.M. (author), Post, S.M. (author), Groenendijk, M. (author), Solaas, K. (author), van der Boom, H. (author), van Duyvenvoorde, W. (author), de Wit, E.C.M. (author), Bloks, V.W. (author), Kuipers, F. (author), Havekes, L.M. (author), Princen, H.M.G. (author), TNO Kwaliteit van Leven (author)The present study was designed to investigate the lipid-lowering properties and mechanisms of action of a new HMG-CoA reductase inhibitor, rosuvastatin, in female ApoE*3-Leiden transgenic mice. Mice received a high fat/cholesterol (HFC) diet containing either rosuvastatin (O [control], 0.00125%, 0.0025%, or 0.005% [w/w]) or 0.05% (w/w)...article 2005
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Lüchtenborg, M. (author), Weijenberg, M.P. (author), Kampman, E. (author), van Muijen, G.N. (author), Roemen, G.M.J.M. (author), Zeegers, M.P.A. (author), Goldbohm, R.A. (author), van't Veer, P. (author), de Goeij, A.F.P.M. (author), van den Brandt, P.A. (author), TNO Kwaliteit van Leven (author)The contribution of cigarette smoking to sporadic colorectal cancer may differ according to molecular aspects of the tumor or according to glutathione S-transferase M1 (GSTM1) or glutathione S-transferase T1 (GSTT1) genotype. In the prospective Netherlands Cohort Study on Diet and Cancer, adjusted incidence rate ratios for 1986-1993 were...article 2005
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van den Akker-van Marle, M.E. (author), Rijnders, M.E.B. (author), van Dommelen, P. (author), Fekkes, M. (author), van Wouwe, J.P. (author), Amelink-Verburg, M.P. (author), Verkerk, P.H. (author)Objective: To estimate the costs and effects of different treatment strategies with intrapartum antibiotic prophylaxis to prevent early-onset group B streptococcal (GBS) disease in the Netherlands. The treatment strategies include a risk-based strategy, a screening-based strategy, a combined screening/risk-based strategy and the current Dutch...article 2005
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Joosten, V. (author), Roelofs, M.S. (author), van den Dries, N. (author), Goosen, T. (author), Verrips, C.T. (author), van den Hondel, C.A.M.J.J. (author), Lokman, B.C. (author), TNO Kwaliteit van Leven (author)The Arthromyces ramosus peroxidase gene (arp) was genetically fused to either the 5′- or 3′-terminal ends of the gene encoding llama variable heavy chain antibody fragment VHH R9, resulting in the fusion expression cassettes ARP-R9 or R9-ARP. Aspergillus awamori transformants were obtained which produced up to 30 mg l-1 fusion protein in the...article 2005
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Venema, K. (author), Vermunt, S.H.F. (author), Brink, E.J. (author), TNO Kwaliteit van Leven (author)D-Tagatose is partly absorbed in the stomach and small intestine. Most of it is fermented by the large intestinal microbiota. The effect of D-tagatose on the composition of the microbiota and production of short chain fatty acids (SCFAs) was studied in vivo and in vitro. Gastrointestinal (GI) complaints were also studied. The in vivo study was...article 2005
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Monraats, P.S. (author), Pires, N.M.M. (author), Schepers, A. (author), Agema, W.R.P. (author), Boesten, L.S.M. (author), de Vries, M.R. (author), Zwinderman, A.H. (author), de Maat, M.P.M. (author), Doevendans, P.A.F.M. (author), de Winter, R.J. (author), Tio, R.A. (author), Waltenberger, J. (author), 't Hart, L.M. (author), Frants, R.R. (author), Quax, P.H.A. (author), van Vlijmen, B.J.M. (author), Havekes, L.M. (author), van der Laarse, A. (author), van der Wall, E.E. (author), Jukema, J.W. (author), TNO Kwaliteit van Leven (author)Genetic factors appear to be important in the restenotic process after percutaneous coronary intervention (PCI), as well as in inflammation, a pivotal factor in restenosis. TNFα, a key regulator of inflammatory responses, may exert critical influence on the development of restenosis after PCI. The GENetic DEterminants of Restenosis (GENDER)...article 2005
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TNO Kwaliteit van Leven (author), Grefhorst, A. (author), van Dijk, T.H. (author), Hammer, A. (author), van der Sluijs, F.H. (author), Havinga, R. (author), Havekes, L.M. (author), Romijn, J.A. (author), Groot, P.H. (author), Reijngoud, D.J. (author), Kuipers, F. (author)Liver X receptor (LXR) agonists have been proposed to act as anti-diabetic drugs. However, pharmacological LXR activation leads to severe hepatic steatosis, a condition usually associated with insulin resistance and type 2 diabetes mellitus. To address this apparent contradiction, lean and ob/ob mice were treated with the LXR agonist GW-3965 for...article 2005
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Joosten, V. (author), Gouka, R.J. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Lokman, B.C. (author), TNO Kwaliteit van Leven TNO Voeding (author)We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were...article 2005
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Damveld, R.A. (author), van Kuyk, P.A. (author), Arentshorst, M. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven TNO Voeding (author)1,3-α-d-Glucan is an important component of the cell wall of filamentous fungi. We have identified a family of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger. The agsA gene was sequenced and the predicted protein sequence indicated that the overall domain structure of 1,3-α-d-glucan synthases is conserved in fungi. Using RT-PCR...article 2005
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TNO Kwaliteit van Leven (author), Espirito Santo, S.M.S. (author), Rensen, P.C.N. (author), Goudriaan, J.R. (author), Bensadoun, A. (author), Bovenschen, N. (author), Voshol, P.J. (author), Havekes, L.M. (author), van Vlijmen, B.J.M. (author)The very low density lipoprotein receptor (VLDLR), low density lipoprotein receptor (LDLR), and low density lipoprotein receptor-related protein (LRP) are the three main apolipoprotein E-recognizing endocytic receptors involved in the clearance of triglyceride (TG)-rich lipoproteins from plasma. Whereas LDLR deficiency in mice results in the...article 2005
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TNO Kwaliteit van Leven (author), Wierckx, N.J.P. (author), Ballerstedt, H. (author), Bont, J.A.M.de (author), Wery, J. (author)Efficient bioconversion of glucose to phenol via the central metabolite tyrosine was achieved in the solvent-tolerant strain Pseudomonas putida S12. The tpl gene from Pantoea agglomerans, encoding tyrosine phenol lyase, was introduced into P. putida S12 to enable phenol production. Tyrosine availability was a bottleneck for efficient production....article 2005
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Verhoeckx, K.C.M. (author), Bijlsma, S. (author), de Groene, E.M. (author), Witkamp, R.F. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), TNO Voeding (author)The monocyte-like human histiocytic lymphoma cell line U937 can be induced by phorbol 12-myristate 13-acetate (PMA) to undergo differentiation into a macrophage-like phenotype. We have used two-dimensional gel electrophoresis (2-DE), oligonucleotide microarrays and principal component analysis (PCA) to characterize the U937 cell line as a model...article 2004
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Lindeman, J.H.N. (author), Hanemaaijer, R. (author), Mulder, A. (author), Dijkstra, P.D.S. (author), Szuhai, K. (author), Bromme, D. (author), Verheijen, J.H. (author), Hogendoorn, P.C.W. (author)Giant cell tumor (GCT) of bone is a neoplasm of bone characterized by a localized osteolytic lesion. The nature of GCT is an enigma and the cell type(s) and protease(s) responsible for the extensive localized clinicoradiological osteolysis remain unresolved. We evaluated protease expression and cellular distribution of the proteolytic machinery...article 2004
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Majhenič, A.Č. (author), Venema, K. (author), Allison, G.E. (author), Matijašić, B.B. (author), Rogelj, I. (author), Klaenhammer, T.R. (author), TNO Voeding (author)Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing analysis revealed the gene of acidocin LF221 A to...article 2004
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Lüchtenborg, M. (author), Weijenberg, M.P. (author), Roemen, G.M.J.M. (author), de Bruïne, A.P. (author), van den Brandt, P.A. (author), Lentjes, M.H.F.M. (author), Brink, M. (author), van Engeland, M. (author), Goldbohm, R.A. (author), de Goeij, A.F.P.M. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)The adenomatous polyposis coli (APC) gene is considered to be a gatekeeper in colorectal tumourigenesis. Inactivating mutations in APC have been reported in 34-70% of sporadic colorectal cancer patients, the majority of which occur in the mutation cluster region (MCR). In this study, tumour tissue from 665 incident colorectal cancer patients,...article 2004
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Characterization of Pea Vicilin. 1. Denoting Convicilin as the α-Subunit of the Pisum Vicilin FamilyO'Kane, F.E. (author), Happe, R.P. (author), Vereijken, J.M. (author), Gruppen, H. (author), van Boekel, M.A.J.S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Vicilin, a major globulin protein of pea that has been described as "extremely heterogeneous in terms of its polypeptide composition", was extracted from pea flour under alkaline conditions and subsequently fractionated by salt under acid conditions. This procedure induced the separation of vicilin into two fractions, which, after purification,...article 2004