Searched for: subject:"Ceramidase"
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document
Houben, E. (author), Uchida, Y. (author), Nieuwenhuizen, W.F. (author), de Paepe, K. (author), Vanhaecke, T. (author), Holleran, W.M. (author), Rogiers, V. (author), TNO Kwaliteit van Leven (author)
It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their...
article 2007
document
Nieuwenhuizen, W.F. (author), van Leeuwen, S. (author), Jack, R.W. (author), Egmond, M.R. (author), Götz, F. (author)
Ceramidase (CDase) hydrolyzes the amide bond in ceramides to yield free fatty acid and sphingosine. From a 3-L Pseudomonas aeruginosa PA01 culture, 70 μg of extracellular alkaline, Ca2+-dependent CDase, was purified to homogeneity, the N-terminal sequence was determined, and the CDase gene was cloned. The CDase gene encodes a 670 amino acid...
article 2003