Tissue factor pathway inhibitor in endothelial cells colocalizes with glycolipid microdomains/caveolae: Regulatory mechanism(s) of the anticoagulant properties of the endothelium

Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...

An endothelial storage granule for tissue-type plasminogen activator

In previous studies we have shown that, after stimulation by a receptor ligand such as thrombin, tissue-type plasminogen activator (tPA) and von Willebrand factor (vW(f)) will be acutely released from human umbilical vein endothelial cells (HUVEC). However, the mechanisms involved in the secretion of these two proteins differ in some respects,...

Enhanced susceptibility of low-density lipoproteins to oxidation in coronary bypass patients with progression of atherosclerosis

Oxidation of low-density lipoprotein (LDL) may play a causal role in atherosclerosis. In this study we analyzed whether the severity of progression of coronary atherosclerosis is related to the susceptibility of LDL to oxidative modification. On the basis of repeated coronary angiography, 28 coronary bypass patients were divided into two groups:...

Changes in the sequence content of albumin mRNA and in its translational activity in the rat liver with age

To investigate the regulation of age-related changes in albumin synthesis in the rat liver, total postnuclear RNA and polyribosomes, both membrane-bound and free, were prepared from livers of rats of different ages. By the use of a specific complementary DNA probe, the albumin mRNA sequence content was quantitated in these RNA fractions. These...

Complications in the determination of HDL2/HDL3 ratios

We investigated different procedures to subfractionate high density lipoprotein (HDL) into its density subfractions (HDL2 and HDL3). When pore-limit as well as zone polyacrylamide gel electrophoresis was used, it proved to be necessary to use freshly prepared and plasma protein-free HDL preparations. For a quantitative subfractionation of HDL in...

The induction of gamma-endonuclease susceptible sites by ??-rays in CHO cells and their cellular repair are not affected by the presence of thiol compounds during irradiation

Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Endonucleases, EC 3.1.-; Sulfhydryl Compounds

Mechanisms of inactivation of bacteriophage phiX174 and its DNA in aerosols by ozone and ozonized cyclohexene

The mechanisms of inactivation of aerosolized bacteriophage phiX174 in atmospheres containing ozone, cyclohexene, or ozonized cyclohexene were studied by using 32P-labelled phage. The inactivation of the aerosolized phage in clean air or in air containing cyclohexene is due to damage of the protein coat since the deoxyribonucleic acid (DNA)...

Isolation and characterization of Kupffer and endothelial cells from the rat liver

Non-parenchymal cell suspensions were prepared from rat livers by three different methods based on a collagenase, a pronase and a combined collagenase-pronase treatment. The highest yield of Kupffer and endothelial cells was obtained with the pronase treatment. Attempts were made for a further purification of these cells by Metrizamide density...

The effect of irradiation with ultraviolet light on the sedimentation behaviour of PM2 DNA

Chemicals/CAS: DNA, Viral

Subcellular localization of newly formed [3H]acetylcholine in rat cerebral cortex in vitro

Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...

Identification of cells in primate bone marrow resembling the hemopoietic stem cell in the mouse

The colony forming unit culture (CFU C) in the thin layer agar colony technique is considered to be representative for hemopoietic stem cells (HSC), according to studies in mouse and monkey bone marrow. Using this in vitro assay as a guide, stem cell concentrates were prepared from monkey and human bone marrow by repeated density gradient...

Repair of X-ray damage in DNA of cultivated cells from patients having xeroderma pigmentosum

Repair replication and rejoining of single-strand breaks after X-irradiation in human-skin fibroblasts from normal donors and several patients with xeroderma pigmentosum have been compared. The xeroderma strains showed different levels of repair replication following UV exposure. Repair replication and rejoining of breaks, which are considered...

On the origin of the 70 S component of bacteriophage ∅X174