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Schuit, H.R.E. (author), Hijmans, W. (author), Instituut voor experimentele gerontologie TNO (author)The presence of surface-associated immunoglobulins and Fc receptors on mononuclear cells from normal human blood waas investigated by the direct immunofluorescence technique combined with phase-contrast microscopy. Formaldehyde-fixed cells were compared to unfixed cells and to cells preincubated at 37??C. In the unfixed samples a separate...article 1980
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Instituut voor experimentele gerontologie TNO (author), Brouwer, A. (author), Wanson, J.C. (author), Mosselmans, R. (author)Adult rat liver sinusoidal cells were isolated by enzymatic perfusion and incubation of the liver, and cultured in Dulbecco's medium supplemented with 17% fetal calf serum. The structure of the cultured cells was studied after various incubation periods by means of phase contrast microscopy and transmission electron microscopy. Cultured sinusoid...article 1980
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Knook, D.L. (author), Sleyster, E.C. (author), Teutsch, H.F. (author), Instituut voor Experimentele Gerontologie TNO (author)Chemicals/CAS: glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; Glucosephosphate Dehydrogenase, EC 1.1.1.49article 1980
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Plooy, A.C.M. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Ten platinum (Pt) coordination complexes with different ligands, comprising both Pt(II) and Pt(IV) complexes of which the cis-compounds all possessed at least some anti-tumour activity and the trans-compounds were inactive, were tested as to their effect on cell survival and the induction and repair of DNA damage in cultured Chinese hamster...article 1980
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Gaubius instituut TNO (author), Kempen, H.J.M. (author)Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1-3 hr) incubation periods. The heaptocytes release very low density (91.10 g/ml). These particles with higher densities have not been positively identified; the latter group is dissimilar to the high density lipoprotein, which occurs in rat liver...article 1980
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- Radiobiologisch Instituut TNO (author), Barendsen, G.W. (author) article 1980
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Radiobiologisch Instituut TNO (author), Kal, H.B. (author), Barendsen, G.W. (author)Chemicals/CAS: vinblastine, 865-21-4; Vinblastine, 865-21-4article 1980
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- Radiobiologisch Instituut TNO (author), Hermens, A.F. (author) article 1980
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Medisch Biologisch Laboratorium TNO (author), Hooisma, J. (author), Groot, D.M.G.de (author), Magchielse, T. (author), Muijser, H. (author)Chick spinal ganglia, chick muscle cells combined with mouse spinal cord explants, C1300 neuroblastoma cells, Chinese hamster ovary cells and newborn rat cerebral cells were exposed to various concentrations of acrylamide in culture. Four morphological and 1 electrophysiological parameter were applied in order to score toxic effects. It appeared...article 1980
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Nederlands Instituut voor Praeventieve Gezondheidszorg TNO (author), Blom, J.L. (author), Barth, P.G. (author), Visser, S.L. (author)With routine techniques the flash-evoked VEPs in 11 consecutive age groups between 0-6 years, each consisting of 8 children, were measured under clinical circumstances. The children were carefully screened to fit within predetermined normal limits. The main results are: (1) The primary part, consisting of 3 components, is well discernible at...article 1980
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Westenbrink, F. (author), Koornstra, W. (author), Creemers, P. (author), Radiobiologisch Instituut TNO (author)Antisera raised in rabbits against purified components gp52, p28 and p12 of murine mammary tumor virus (MuMTV) were first characterized by means of immunodiffusion, immunoelectrophoresis and radioimmunoassay. No significant crossreactivity could be detected. The 3 polypeptides were detected by means of these antisera in the cytoplasm of murine...article 1979
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Russell, J.L. (author), van den Engh, G.J. (author), Radiobiologisch Instituut TNO (author)Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. Of antisera which are specific for subregions of the H-2 complex, only those which contain anti-H-2K or anti-H-2D activity are effective. Specific anti-Ia sera do not react with the stem cell. If...article 1979
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Reimann, J. (author), Burger, H. (author), Radiobiologisch Instituut TNO (author)The culture system, in which a marrow-derived adherent cell population, established in vitro, exerts a long-term promoting influence on proliferation of haemopoietic cells, is reproduced. Essential parameters of the system are investigated; it is confirmed that the system is critically dependent on horse serum, and on the in vitro age of the...article 1979
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Radl, J. (author), de Glopper, E. (author), Schuit, H.R.E. (author), Zurcher, C. (author), Instituut voor experimentele gerontologie TNO (author)Transplantation experiments in the C57BL/KaLwRij mouse model of idiopathic paraproteinemia (IP) showed that an IP-producing clone can be further propagated in young, lethally irradiated mice and also equally as well in nonirradiated recipients by a bone marrow and/or spleen cell transfer. The latency period before the original paraprotein was...article 1979
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Reynolds, R.J. (author), Natarajan, A.T. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: endonuclease, 9055-11-2; Endonucleases, EC 3.1.article 1979
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Kruisbeek, A.M. (author), Instituut voor experimentele gerontologie TNO (author)In the present review the results obtained so far with thymic epithelial culture supernatants (TES) or cocultivation of potential precursor T cells with TE cells will be discussed and compared with the effects reported for other thymic factors in studies on T cell differentiation in vitro. These studies can be divided into those involved with T...article 1979
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Gaillard, A.W.K. (author), Varey, C.A. (author), Instituut voor Zintuigfysiologie TNO (author)The effects (on human beings) of the ACTH analog Org 2766 were investigated for a range of performance tests: complicated serial reaction task, running memory span, verbal learning and non-verbal mental ability tests (closure flexibility and non-verbal abstraction). Subjective ratings on feeling were taken and heart rate was measured. Only the...article 1979
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van Bekkum, D.W. (author), van den Engh, G.J. (author), Wagemaker, G. (author), Radiobiologisch Instituut TNO (author)A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. This entity was subsequently discovered in concentrated HSC preparations from bone marrow of rats, monkeys, and humans. In the mouse, a set of physical...article 1979
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Perdok, J. (author), Gaillard, A.W.K. (author), Instituut voor Zintuigfysiologie TNO (author)Slow EEG potentials were recorded during the foreperiod of a signal detection task which involved different levels of discrimination difficulty. The subject was requried to react to S2 as quickly as possible or to delay his responses by 1 s. It was found that the contingent negative variation (CNV) was attenuated when no speeded response was...article 1979
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van der Schans, G.P. (author), Centen, H.B. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Endonucleases, EC 3.1.-; Sulfhydryl Compoundsarticle 1979