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Concomitant increase in hepatic triacylglycerol biosynthesis and cytosolic fatty-acid-binding-protein content after feeding rats with a cholestyramine-containing diet

Gaubius instituut TNO (author), Kempen, H.J.M. (author), Glatz, J.F.C. (author), Lange, J.de (author), Veerkamp, J.H. (author)

Cholestyramine feeding of rats increased the rate of palmitate and glycerol incorporation into triacylglycerols of isolated hepatocytes. Concomitantly an increase of fatty-acid binding by hepatic cytosolic proteins was observed, which could be attributed to an elevation of the content of the fatty-acid-binding protein (M(r) 12,000). The...

article 1983

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Age-related changes in albumin elimination in female WAG/Rij rats

Instituut voor experimentele gerontologie TNO (author), Horbach, G.J.M.J. (author), Yap, S.H. (author), van Bezooijen, C.F.A. (author)

Albumin elimination rates were determined in 3-, 12-, 24- and 36-month-old female WAG/Rij rats. No change in elimination half-life was found with age. However, as there was an increase in the whole-body albumin pool, a concomitant increase in albumin clearance was observed at between 12 and 36 months of age. It was concluded that the increase in...

article 1983

document

Bile acids and lipids in isolated rat hepatocytes. II. Source of cholesterol used for bile acid formation, estimated by incorporation of tritium from tritiated water, and by the effect of ML-236B

Gaubius instituut TNO (author), Kempen, H.J. (author), Vos Van Holstein, M. (author), Lange, J.de (author)

Chemicals/CAS: cholesterol, 57-88-5; cholic acid, 32500-01-9, 361-09-1, 81-25-4; colestyramine, 11041-12-6, 58391-37-0; compactin, 73573-88-3; lipid, 66455-18-3; tritium oxide, 14940-65-9; Bile Acids and Salts; Cholesterol, 57-88-5; Cholestyramine, 11041-12-6; compactin, 73573-88-3; Lipids; Naphthalenes; Tritium, 10028-17-8

article 1983

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Meclofenoxate decreases protein and albumin synthesis in hepatocytes isolated from old rats

Instituut voor experimentele gerontologie TNO (author), van Bezooijen, C.F.A. (author), Sakkee, A.N. (author), Knook, D.L. (author)

Protein-synthesizing activity was studied in hepatocytes isolated from rats of various ages with or without treatment with 80 mg meclofenoxate/day per kg body weight for 2 weeks. The substance did not influence protein synthesis in hepatocytes isolated from 3- and 12-month-old rats, but drastically decreased the synthesis of protein by...

article 1983

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A familial hemorrhagic diathesis in a Dutch family: An inherited deficiency of ??2-antiplasmin

Kluft, C. (author), Vellenga, E. (author), Brommer, E.J.P. (author), Wijngaards, G. (author), Gaubius instituut TNO (author)

This study concerns a case of congenital homozygous deficiency in ??2-antiplasmin associated with a severe hemorrhagic diathesis. Heterozygous family members also show a mild bleeding tendency. The propositus is a 17-yr-old male born of white parents and showing a severe hemorrhagic diathesis characterized by spontaneous bleeding in the joints...

article 1982

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The effect of X-rays on the precursors of antibody forming cells (B cells) as measured with the in vitro limiting dilution assay

Lubbe, F.H. (author), Hooijkaas, H. (author), Preesman, A.A. (author), Medisch Biologisch Laboratorium TNO (author)

The effect of X-irradiation upon murine antibody-forming cell precursors (B cells) was established in cultures of spleen cells stimulated with the B cell mitogen lipopolysaccharide (LPS). At day 5 and 7 the numbers of IgM- and IgG2-secreting cells were determined in cultures of irradiated and nonirradiated spleen cells. From these numbers a D(o)...

article 1982

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Molecular dosimetry of DNA damage caused by alkylation. I. Single-strand breaks induced by ethylating agents in cultured mammalian cells in relation to survival

Abbondandolo, A. (author), Dogliotti, E. (author), Lohman, P.H.M. (author), Berends, F. (author), Medisch Biologisch Laboratorium TNO (author)

Cultured Chinese hamster ovary cells were treated with ethylating agents. DNA lesions giving rise to single-strand breaks (ssb) or alkali-labile sites were measured by centrifugation in alkaline sucrose gradients after lysis in alkali. 4 agents with different tendencies to ethylate preferentially either at N or O atoms were compared, namely N...

article 1982

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Reduction of polyneuronal innervation of muscle cells in tissue culture after long-term indirect stimulation

Magchielse, T. (author), Meeter, E. (author), Medisch Biologisch Laboratorium TNO (author)

In combined cultures of chick muscle cells and ciliary ganglia, the effect of indirect, long-term stimulation on the number of neuromuscular contacts on individual muscle cells was studied. Seven days of phasic stimulation resulted in a marked reduction in the number of neuromuscular contacts, the majority of the muscle cells becoming innervated...

article 1982

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Contrast paradox of line target upon grating background: Letter to the Editor

van Meeteren, A. (author), Instituut voor Zintuigfysiologie TNO (author)

article 1982

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Tissue culture and neurotoxicology

Hooisma, J. (author), Medisch Biologisch Laboratorium TNO (author)

Application of tissue culture in neurotoxicology may serve two purposes. First, they may be used to unravel the mechanism of action of neurotoxic compounds and secondly, they may be used for the screening of neurotoxic agents. Studies belonging to the first group can be subdivided into those aiming at the replication of neurotoxic processes in...

article 1982

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Glucagon receptors: Are they present on human blood cells?

van Hinsbergh, V.W.M. (author), Nooijen, W.J. (author), Kempen, H.J.M. (author), Gaubius instituut TNO (author)

Binding of glucagon to and its degradation by human mononuclear leukocytes and erythrocytes was investigated. Labeled glucagon binds to these cells, but can be displaced only by unlabeled glucagon at pharmacologically high concentrations. It is concluded that glucagon binding to human blood cells does not represent receptors of physiological...

article 1982

document

The effect of irradiation on the antibody enhancing helper T cells

Lubbe, F.H. (author), Brocades Zaalberg, O. (author), Medisch Biologisch Laboratorium TNO (author)

Helper T cells specific for sheep erythrocytes were generated in lethally irradiated mice. The helper effect exerted by these cells was determined in vitro in spleen cell cultures. Irradiation of the helper cells in vivo or in vitro with 1-9 Gy resulted in a bimodal relation between radiation dose and helper effect when measured either...

article 1982

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Absence of an effect of lead acetate on sperm morphology, sister chromatid exchanges or on micronuclei formation in rabbits

Willems, M.I. (author), de Schepper, G.G. (author), Wibowo, A.A.E. (author), Immel, H.R. (author), Dietrich, A.J.J. (author), Zielhuis, R.L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

The influence of lead on sperm morphology, sister chromatid exchanges or on micronuclei formation was studied on male rabbits after exposure to doses of 0, 0.25, and 0.50 mg lead acetate/kg body weight subcutaneously injected three times a week during 14 weeks, each on a group of five rabbits. At the end of exposure phase the lead in blood...

article 1982

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Determination of the B2 vitamer flavin-adenine dinucleotide in whole blood by high-performance liquid chromatography with fluorometric detection

Speek, A.J. (author), van Schaik, F. (author), Schrijver, J. (author), Schreurs, W.H.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A reliable high-performance liquid chromatographic (HPLC) method is developed for the analysis of FAD in whole blood of humans. The method is able to separate FAD, FMN and Rb from each other and from interfering compounds. A reliable and sensitive detection of FAD has been obtained by selecting the optimal pH of the mobile phase and optimal...

article 1982

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Influence of adrenoceptor blockade on endotoxin-induced histopathological changes in murine Meth A sarcoma

Kuper, F. (author), Bloksma, N. (author), Hofhuis, F.M. (author), Bruyntjes, J.P. (author), Willers, J.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Histological examination of subcutaneous murine Meth A sarcoma 4 h after administration of endotoxin revealed an overt but superficial vasodilation, which had largely disappeared after 24 h. At this time hemorrhagic necrosis could be observed in that region mainly featured by degenerating tumour cells, diffuse hemorrhage and blood vessels filled...

article 1982

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Nature and histogenesis of sulfite-induced gastric lesions in rats

Beems, R.B. (author), Spit, B.J. (author), Koeter, H.B.W.M. (author), Feron, V.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A variety of gastric lesions can be induced in rats by sodium metabisulfite, including the formation of scattered hyperplastic glands in the fundic mucosa which are exclusively lined by large uniform cells, laden with acidophilic granules. Light and electron microscopy, as enzyme histochemistry, showed that the cells lining these glands are...

article 1982

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Bile acids and lipids in isolated rat hepatocytes: Content, synthesis, and release, as affected by cholestyramine treatment of the donor rats

Gaubius instituut TNO (author), Kempen, H.J.M. (author), van Vos-Holstein, P.M. (author), de Lange, J. (author)

Contents of bile acids and lipids, as well as rates of triglyceride synthesis, were determined in isolated hepatocytes from control or cholestyramine-fed rats (denoted below as "control" or "treated" hepatocytes, respectively). During a 3-hr incubation period, total bile acid production was markedly higher in "treated" cells than in "conrol"...

article 1982

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Quantitative determination of in vivo endocytosis by rat liver Kupffer and endothelial cells facilitated by an improved cell isolation method

Instituut voor experimentele gerontologie TNO (author), Praaning-Van Dalen, D.P. (author), Knook, D.L. (author)

Chemicals/CAS: Serum Albumin, Bovine

article 1982

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Characterisation of mouse prothymocytes

Radiobiologisch instituut TNO Instituut voor experimentele gerontologie TN (author), Boersma, W.J.A. (author)

In this thesis, the study of growth kinetics of thymocytes in lethally irradiated mice as a means to characterise the early T-cell progenitors is described. Normal bone marrow cells were used as the source of hemopoietic cells. The experiments show that it is possible to obtain information on T-cell differentiation from a complex experimental...

doctoral thesis 1982

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N-alkane neurotoxicity in cultured cerebral cells

Hooisma, J. (author), Dam, E.W. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: 2 hexanone, 591-78-6; acetonylacetone, 110-13-4; hexane, 110-54-3

article 1981