Localization of murine mammary tumor virus polypeptides on the surface of tumor cells

Antisera raised in rabbits against purified components gp52, p28 and p12 of murine mammary tumor virus (MuMTV) were first characterized by means of immunodiffusion, immunoelectrophoresis and radioimmunoassay. No significant crossreactivity could be detected. The 3 polypeptides were detected by means of these antisera in the cytoplasm of murine...

Micrococcus luteus UV-endonuclease-sensitive sites and sister-chromatid exchanges in Chinese hamster ovary cells

Chemicals/CAS: endonuclease, 9055-11-2; Endonucleases, EC 3.1.

The induction of gamma-endonuclease susceptible sites by ??-rays in CHO cells and their cellular repair are not affected by the presence of thiol compounds during irradiation

Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Endonucleases, EC 3.1.-; Sulfhydryl Compounds

Repair of UV-endonuclease-susceptible sites in the 7 complementation groups of xeroderma pigmentosum A through G

Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Pyrimidine Dimers

The role of fibroblastoid cells and macrophages from mouse bone marrow in the in vitro growth promotion of haemopoietic tumour cells

Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. The latter were characterized by means of cell density...

In vitro evaluation of some latent radioprotective compounds

In tissue culture, protection against X irradiation by a number of cysteamine derivatives was studied and the results were compared with data obtained in mice. Compounds with a covered SH group, like WR 638, cysteamine phosphate, WR 2721 and AE AE 48527, showed practically no protection when dissolved in tissue culture medium, but developed a...

Radiosensitization of mammalian cells by diamide

The effect of diamide on the radiosensitivity of T cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose modifying factors of 0.81, 0.60 and 0.55,...

Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity

The PGK activity was assayed in diploid, hyperdiploid, tetraploid, and hybrid cells all originating from the same Chinese hamster cell line (DON line). A relationship between gene multiplicity and enzyme activity was observed. Selective pressure on the HGPRT locus by growth of hybrid cells in the presence of 8-azaguanine resulted in decreased...