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Hermens, A.F. (author), Bentvelzen, P.A.J. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)Rat R2k rhabdomyosarcoma cells were transfected with the human H-ras oncogene, which resulted in increased resistance to cell kill in vitro by a single dose of 137Cs γ-rays. A subline carrying one oncogene showed an increase in the quasi-threshold dose (D(q)) from 0.88 to 1.48 Gy. Another subline containing six oncogenes not only had an...article 1992
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van Oers, M.M. (author), Malarme, D. (author), Jore, J.P.M. (author), Vlak, J.M. (author), Medisch Biologisch Laboratorium TNO (author)Two major late proteins, polyhedrin and p10, are synthesized in large quantities in baculovirus infected insect cells. This and the fact that both proteins are dispensable for virus replication, form the basis for the use of these viruses as vector for foreign gene expression. To address the question whether the Autographa californica nuclear...article 1992
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Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...article 1991
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Mulder, M. (author), de Wit, E. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)It has been suggested that besides the LDL-receptor, hepatocytes possess an apo E or remnant receptor. To evaluate which hepatic lipoprotein receptor is involved in VLDL remnant catabolism, we studied the binding of VLDL remnants to HepG2 cells. Native VLDL was obtained from type IIb hyperlipidemic patients and treated with bovine milk...article 1991
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Burger, H. (author), van Leen, R.W. (author), Dorssers, L.C.J. (author), Persoon, N.L.M. (author), Lemson, P.J. (author), Wagemaker, G. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)To enable preclinical studies on homologous interleukin-3 (IL-3) in primate species, we isolated the gene encoding Rhesus monkey IL-3 (RhIL-3). The nucleotide sequence of the RhIL-3 gene displayed 92.9% homology with that of the human IL-3 (hIL-3) gene. The isolated RhIL-3 gene encodes a 143-amino acid (aa) precursor polypeptide, nine C-terminal...article 1990
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Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author), van Beusechem, V.W. (author), Kukler, A. (author), Einerhand, M.P.W. (author), Bakx, T.A. (author), van der Eb, A.J. (author), van Bekkum, D.W. (author), Valerio, D. (author)Amphotropic recombinant retroviruses were generated carrying sequences encoding human adenosine deaminase (ADA). Transcription of the human ADA gene was under control of a hybrid long terminal repeat in which the enhancer from the Moloney murine leukemia virus was replaced by an enhancer from the F101 host-range mutant of polyoma virus....article 1990
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Otter, M. (author), van Berkel, T.J.C. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)In this study, binding and degradation of tissue-type plasminogen activator (t-PA) by the human hepatoma cell line Hep G2 was investigated. Binding at 4°C was time-dependent and reached a maximum after ca. 2 hours. Scatchard analysis of saturation experiments showed about 170,000 high affinity binding sites for t-PA per cell with an apparent Kd...article 1989
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Emeis, J.J. (author), Edgell, C.J.S. (author), Gaubius instituut TNO (author)The fibrinolytic characteristics of the endothelial hybrid cell line EA.hy 926, established by fusing a human umbilical vein endothelial cell with a human carcinoma cell line, were studied. The hybrid cell line produced large amounts of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor type 1, and a small amount of...article 1988
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Gaubius instituut TNO (author), Cohen, L.H. (author), Griffioen, M. (author)Hep G2 cells were incubated under conditions known to influence the HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase activity, e.g. in the presence of compactin (a competitive inhibitor of HMG-CoA reductase itself) and U18666A (a squalene-2,3-epoxide cyclase inhibitor). We studied the effects of these conditions both on the HMG-CoA reductase...article 1988
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Gaubius instituut TNO (author), Boogaard, A. (author), Griffioen, M. (author), Cohen, L.H. (author)Incubating Hep G2 cells for 18 h with triparanol, buthiobate and low concentrations (< 0.5 ??M) of U18666A, inhibitors of desmosterol ??24-reductase, of lanosterol 14??-demethylase and of squalene-2,3-epoxide cyclase (EC 5.4.99.7) respectively, resulted in a decrease of the HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase activity....article 1987
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Gaubius instituut TNO (author), Havekes, L.M. (author), de Wit, E.C. (author), Princen, H.M. (author)We have previously shown that in Hep G2 cells and human hepatocytes, as compared with fibroblasts, the low-density lipoprotein (LDL) receptor activity is only weakly down-regulated after incubation of the cells with LDL, whereas incubation with high-density lipoproteins (HDL) of density 1.16-1.20 g/ml (heavy HDL) strongly increased the LDL...article 1987
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- Rutten, A.A.J.J.L. (author), Wilmer, J.W.G.M. (author), Instituut CIVO-Toxicologie en voeding TNO (author) article 1986
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- Instituut CIVO-Toxicologie en Voeding TNO (author), Willems, M.I. (author), de Raat, W.K. (author) article 1985
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Gaubius instituut TNO (author), Cohen, L.H. (author), Griffioen, M. (author), Havekes, L. (author)Compactin, an inhibitor of HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase, decreased cholesterol synthesis in intact Hep G2 cells. However, after the inhibitor was washed away, the HMG-CoA-reductase activity determined in the cell homogenate was found to be increased. Also the high-affinity association of LDL (low-density lipoprotein) to Hep...article 1984
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Gaubius Instituut TNO (author), Kooistra, T. (author), van Hinsbergh, V. (author), Havekes, L. (author), Kempen, H.J. (author)Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein. No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is present on the surface of...article 1984
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Gaubius instituut TNO (author), Havekes, L. (author), van Hinsbergh, V. (author), Kempen, H.J. (author), Emeis, J. (author)The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a K(D) of 25 nM-LDL and a maximal amount of binding of about 70...article 1983
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Abbondandolo, A. (author), Dogliotti, E. (author), Lohman, P.H.M. (author), Berends, F. (author), Medisch Biologisch Laboratorium TNO (author)Cultured Chinese hamster ovary cells were treated with ethylating agents. DNA lesions giving rise to single-strand breaks (ssb) or alkali-labile sites were measured by centrifugation in alkaline sucrose gradients after lysis in alkali. 4 agents with different tendencies to ethylate preferentially either at N or O atoms were compared, namely N...article 1982
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Klein, J.C. (author), Radiobiologisch Instituut TNO (author)In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. Two of them are claimed to be useful as test systems for the screening of new anticancer drugs and even for drug sensitivity testing of individual human tumours in devising individualized cancer chemotherapy regimens. Three assays were...article 1981
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Zelle, B. (author), Berends, F. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)The xeroderma pignemtosum fibroblast strains XP2RO, complementation group E, and XP23OS, group F were compared with normal human primary fibroblasts UV. regard to repair of damage induced by 254-nn UV> In XP2RO cells, repair DNA synthesis, measured by autoradiography (unscheduled DNA synthesis = UDS), was about 50% of the value found in normal...article 1980
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- Radiobiologisch Instituut TNO (author), Hermens, A.F. (author) article 1980