Searched for: subject%3A%22Cell%255C%2Bline%22
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Vogels, R. (author), Zuijdgeest, D. (author), van Rijnsoever, R. (author), Hartkoorn, E. (author), Damen, I. (author), de Béthune, M.-P. (author), Kostense, S. (author), Penders, G. (author), Helmus, N. (author), Koudstaal, W. (author), Cecchini, M. (author), Wetterwald, A. (author), Sprangers, M. (author), Lemckert, A. (author), Ophorst, O. (author), Koel, B. (author), van Meerendonk, M. (author), Quax, P. (author), Panitti, L. (author), Grimbergen, J. (author), Bout, A. (author), Goudsmit, J. (author), Havenga, M. (author)
Replication-deficient human adenovirus type 5 (Ad5) can be produced to high titers in complementing cell lines, such as PER.C6, and is widely used as a vaccine and gene therapy vector. However, preexisting immunity against Ad5 hampers consistency of gene transfer, immunological responses, and vector-mediated toxicities. We report the...
article 2003
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Wortelboer, H.M. (author), Usta, M. (author), van der Velde, A.E. (author), Boersma, M.G. (author), Spenkelink, B. (author), van Zanden, J.J. (author), Rietjens, I.M.C.M. (author), van Bladeren, P.J. (author), Cnubben, N.H.P. (author)
The multidrug resistance proteins MRP1 and MRP2 are efflux transporters with broad substrate specificity, including glutathione, glucuronide, and sulfate conjugates. In the present study, the interaction of the dietary polyphenol curcumin with MRP1 and MRP2 and the interplay between curcumin-dependent MRP inhibition and its glutathione-dependent...
article 2003
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Dommels, Y.E.M. (author), Alink, G.M. (author), van Bladeren, P.J. (author), van Ommen, B. (author), TNO Voeding (author)
During the past few decades, many studies have been conducted to evaluate the effects of n-6 and n-3 polyunsaturated fatty acids (PUFAs) on colorectal carcinogenesis. This report provides a brief overview of the recent studies that have been performed in cultured colon cells, animal models as well as of the population-based and short-term...
article 2002
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Tajima, O. (author), Schoen, E.D. (author), Feron, V.J. (author), Groten, J.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
This paper presents a test strategy to detect interactive effects between several mycotoxins using a DNA synthesis inhibition assay in L929 cells. The joint action of the Fusarium mycotoxins T-2 toxin (T2), deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and fumonisin (FB1) was studied in a tiered approach. In the first stage, the...
article 2002
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Versantvoort, C.H.M. (author), Ondrewater, R.C.A. (author), Duizer, E. (author), van de Sandt, J.J.M. (author), Gilde, A.J. (author), Groten, J.P. (author), TNO Voeding (author)
Purpose: previous studies have shown that the rat small intestinal cell line IEC-18 provides a size-selective barrier for paracellularly transported hydrophilic macromolecules. In order to determine the utility of IEC-18 cells as an in vitro model to screen the passive paracellular and transcellular components of the intestinal transport of...
article 2002
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Gaubius Instituut TNO (author), Grefhorst, A. (author), Elzinga, B.M. (author), Voshol, P.J. (author), Plösch, T. (author), Kok, T. (author), Bloks, V.W. (author), van der Sluijs, F.H. (author), Havekes, L.M. (author), Romijn, J.A. (author), Verkade, H.J. (author), Kuipers, F. (author)
The oxysterol-activated liver X receptor (LXR) provides a link between sterol and fatty acid metabolism; activation of LXR induces transcription of lipogenic genes. This study shows that induction of the lipogenic genes Srebp-1c, Fas, and Acc1 upon administration of the synthetic LXR agonist T0901317 to C57BL/6J mice (10 mg/kg/day, 4 days) is...
article 2002
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Delsing, D.J.M. (author), Offerman, E.H. (author), van Duyvenvoorde, W. (author), van der Boom, H. (author), de Wit, E.C.M. (author), Gijbels, M.J.J. (author), van der Laarse, A. (author), Jukema, J.W. (author), Havekes, L.M. (author), Princen, H.M.G. (author), Gaubius Instituut TNO (author)
Background - The present study investigated whether the ACAT inhibitor avasimibe can reduce atherogenesis independently of its cholesterol-lowering effect in ApoE*3-Leiden mice. Methods and Results - Two groups of 15 female ApoE*3-Leiden mice were put on a high-cholesterol (HC) diet; 1 group received 0.01% (wt/wt) avasimibe mixed into the diet....
article 2001
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Reichl, F.X. (author), Walther, U.I. (author), Durner, J. (author), Kehe, K. (author), Hickel, R. (author), Kunzelmann, K.H. (author), Spahl, W. (author), Hume, W.R. (author), Benschop, H.P. (author), Forth, W. (author)
Objective: The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA), as well as mercuric chloride (HgCl2) and methylmercury chloride (MeHgCl) was investigated on the release of lactatedehydrogenase (LDH) from alveolar epithelial lung cell lines in vitro. Methods: The confluent cell...
article 2001
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), van den Berg, R. (author), Haenen, G.R.M.M. (author), van den Berg, H. (author), Bast, A. (author)
There is increasing interest in the involvement of transcription factors, such as of the transcription factor NF-κB (nuclear factor-κB), in the pathogenesis of various diseases. NF-κB is involved in the control of the transcription of a variety of cellular genes that regulate the inflammatory response by the production of cytokines, chemokines,...
article 2001
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Gaubius Instituut TNO (author), Gervois, P. (author), Vu-Dac, N. (author), Kleemann, R. (author), Kockx, M. (author), Dubois, G. (author), Laine, B. (author), Kosykh, V. (author), Fruchart, J.C. (author), Kooistra, T. (author), Staels, B. (author)
Fibrinogen is a coagulation factor and an acute phase reactant up-regulated by inflammatory cytokines, such as interleukin 6 (IL-6). Elevated plasma fibrinogen levels are associated with coronary heart diseases. Fibrates are clinically used hypolipidemic drugs that act via the nuclear receptor peroxisome proliferator-activated receptor alpha ...
article 2001
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Bogaards, J.J.P. (author), Hissink, E.M. (author), Briggs, M. (author), Weaver, R. (author), Jochemsen, R. (author), Jackson, P. (author), Bertrand, M. (author), van Bladeren, P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A strategy is presented to predict interindividual variation in drug plasma levels in vivo by the use of physiologically based pharmacokinetic modeling and human in vitro metabolic parameters, obtained through the combined use of microsomes containing single cytochrome P450 enzymes and a human liver microsome bank. The strategy, applied to the...
article 2000
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Kappers, W.A. (author), van Och, F.M.M. (author), de Groene, E.M. (author), Horbach, G.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Three different in vitro mutation assays were used to investigate the involvement of cytochrome P450 enzymes in the activation of the nitro- polycyclic aromatic hydrocarbons (nitroPAHs) 1-nitropyrene and 2- nitrofluorene and their reduced metabolites amino-polycyclic aromatic hydrocarbons (aminoPAHs) 1-aminopyrene and 2-aminofluorene....
article 2000
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van Stipdonk, M.J.B. (author), Willems, A.A. (author), Verbeek, R. (author), Boog, C.J.P. (author), van Noort, J.M. (author), TNO Preventie en Gezondheid (author)
The myelin-associated stress protein αB-crystallin triggers strong proliferative responses and IFN-γ production by human T cells and it is considered a candidate autoantigen in multiple sclerosis. In this study we examined the capacity of αB-crystallin or peptides derived thereof to induce experimental autoimmune encephalomyelitis (EAE) in SJL...
article 2000
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Jong, M.C. (author), Hendriks, W.L. (author), van Vark, L.C. (author), Dahlmans, V.E.H. (author), Groener, J.E.M. (author), Havekes, L.M. (author), Gaubius instituut TNO (author)
The present study examined the relative contributions of the different pathways by which oxidatively modified VLDL (oxVLDL) promotes the uptake and intracellular accumulation of lipids in J774 macrophages. VLDL was oxidized for a maximum of 4 hours, resulting in an increase in thiobarbituric acid- reactive substances and an increased...
article 2000
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Abeyama, K. (author), Eng, W. (author), Jester, J.V. (author), Vink, A.A. (author), Edelbaum, D. (author), Cockerell, C.J. (author), Bergstresser, P.R. (author), Takashima, A. (author)
Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation...
article 2000
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Pinelli, E. (author), Gebhard, D. (author), Mommaas, A.M. (author), van Hoeij, M. (author), Langermans, J.A.M. (author), Ruitenberg, E.J. (author), Rutten, V.P.M.G. (author)
We have previously shown that resistance to Leishmania infantum in dogs is associated with a Th1 type of immune response. In this study, we use a canine macrophage cell line (030-D) that can readily be infected with this protozoan parasite. Our aim is to further characterize the effector mechanisms involved in killing of Leishmania parasite in...
article 2000
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de Beer, F. (author), Hendriks, W.L. (author), van Vark, L.C. (author), Kamerling, S.W.A. (author), van Dijk, K.W. (author), Hofker, M.H. (author), Smelt, A.H.M. (author), Havekes, L.M. (author), Gaubius instituut TNO (author)
The binding of β-VLDL to heparan sulfate proteoglycans (HSPG) has been reported to be stimulated by both apoE and lipoprotein lipase (LPL). In the present study we investigated the effect of the isoform and the amount of apoE per particle, as well as the role of LPL on the binding of β-VLDL to HSPG. Therefore, we isolated β-VLDL from transgenic...
article 1999
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Izeboud, C.A. (author), Monshouwer, M. (author), van Miert, A.S.J.P.A.M. (author), Witkamp, R.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Objective and Design: To investigate the suppressive effects of the β-agonist clenbuterol on the release of TNF-α and IL-6 in a lipopolysaccharide (LPS)-model of inflammation, both in vitro and in vivo. Material and Subjects: Human U-937 cell line (monocyte-derived macrophages), and male Wistar rats (200-250 g). Treatment: U-937 macrophages were...
article 1999
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Ordentlich, A. (author), Barak, D. (author), Kronman, C. (author), Benschop, H.P. (author), de Jong, L.P.A. (author), Ariel, N. (author), Barak, R. (author), Segall, Y. (author), Velan, B. (author), Shafferman, A. (author)
The stereoselectivity of the phosphonylation reaction and the effects of adduct configuration on the aging process were examined for human acetylcholinesterase (HuAChE) and its selected active center mutants, using the four stereomers of 1,2,2-trimethylpropyl methylphosphonofluoridate (soman). The reactivity of wild type HuAChE toward the P(S)...
article 1999
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Thole, J.E.R. (author), Janson, A.A.M. (author), Cornelisse, Y. (author), Schreuder, G.M.T. (author), Wieles, B. (author), Naafs, B. (author), de Vries, R.R.P. (author), Ottenhoff, T.H.M. (author), Gaubius instituut TNO (author)
The recognition of 16 mycobacterial Ags by a panel of T cell lines from leprosy patients and healthy exposed individuals from an endemic population was examined within the context of expressed HLA-DR molecules. Although overall no significant differences were found between the frequencies of Ag recognition in the different subject groups, when...
article 1999
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