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van der Schans, G.P. (author), Centen, H.B. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Endonucleases, EC 3.1.-; Sulfhydryl Compoundsarticle 1979
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Rijken, D.C. (author), Wijngaards, G. (author), Zaal-de Jong, M. (author), Welbergen, J. (author), Gaubius instituut TNO (author)A procedure was developed for the purification of a plasminogen activator from human uterine tissue. It involves six consecutive steps: extraction of the plasminogen activator from delipidated uterine tissue with 0.3 M potassium acetate buffer, pH 4.2; ammonium sulphate precipitation; zinc chelate-agarose chromatography; n-butyl-agarose...article 1979
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Slaaf, D.W. (author), Hooisma, J. (author), Meeter, E. (author), Stevens, W.F. (author), Medisch Biologisch Laboratorium TNO (author)Electrophysiological and pharmacological properties of neuromuscular junctions formed in tissue culture between chick ciliary ganglia and chick skeletal muscle cells have been studied. Functional neuromuscular junctions are formed already within 24h. No functional acetylcholine (ACh) esterase is present at these endplates. The neurites conduct...article 1979
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Kal, H.B. (author), Barendsen, G.W. (author), Janse, H.C. (author), Radiobiologisch Instituut TNO (author)The interaction of irradiation and vinblastine was investigated in a solid rat rhabdomyosarcoma. Growth delay of the tumor and cell survival by an in vitro assay were the endpoints measured. Vinblastine was administered at a dose of 1.5 mg/kg body weight at different time intervals before or after doses of 10 and 20 Gy of X-rays. Excess growth...article 1979
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Zelle, B. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Pyrimidine Dimersarticle 1979
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Zipori, D. (author), Bol, S. (author), Radiobiologisch Instituut TNO (author)Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. The latter were characterized by means of cell density...article 1979
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Reimann, J. (author), Burger, H. (author), Radiobiologisch Instituut TNO (author)Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. Medium,...article 1979
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Vos, O. (author), Wilschut, I.J.C. (author), Medisch Biologisch Laboratorium TNO (author)Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after...article 1979
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Gaubius Instituut TNO (author), Nieuwenhuizen, W. (author), Vermond, A. (author), Nooijen, W.J. (author), Haverkate, F. (author)Here the results obtained with human fibrinogen and its degradation products are reported. Our results differ from those obtained in (9) but strongly support model suggested earlier by us for Ca2(+)-binding by rat fibrinogen. Chemicals/CAS: calcium, 7440-70-2; fibrin, 9001-31-4; fibrinogen, 9001-32-5; Calcium, 7440-70-2; Fibrin, 9001-31-4;...article 1979
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Gezondheidsorganisatie TNO Gaubius Instituut TNO (author), Hoekstra, W.P.M. (author), Havekes, A.M. (author)To study the role of the E. coli recipient cell in conjugation recipient cell mutants deficient in conjugation (Con-) were isolated. Mutants specific for F-type E. coli donor cells (ConF-) and mutants specific deficient in conjugation with I-type donor cells (ConI-) were isolated. Both ConF- and ConI- mutants were blocked in stable mating pair...article 1979
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Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)Ageing is associated with declines in the functional capacities of several organs. General causes for the decline can be divided into: 1. intrinsic cellular causes and 2. extracellular causes, e.g., changes in blood circulation and distribution. For the first group of causes, there is evidence for a decrease in the numbers of long-lived cells in...article 1978
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Vos, O. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: Lipopolysaccharidesarticle 1978
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Hooisma, J. (author), Slaaf, D.W. (author), Meeter, E. (author), Stevens, W.F. (author), Medisch Biologisch Laboratorium TNO (author)The influence of various neural explants on the morphology and the survival of chick muscle fibers was studied. A method was developed to evaluate the condition of the muscle fibers using the following four morphological parameters: cross striation, thickness, number of fibers and absence of vacuoles. Chick as well as mouse spinal cord explants...article 1978
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Stevens, W.F. (author), Slaaf, D.W. (author), Hooisma, J. (author), Magchielse, T. (author), Meeter, E. (author), Medisch Biologisch Laboratorium TNO (author)A less complicated source of neurons suitable for this type of studies is the parasympathetic ciliary ganglion. In the pigeon and in the chick this ganglion is known to contain only two classes of neurons, both of which are cholinoceptive and cholinergic and that innervate the muscle fibres of the choroid plexus of the eye, the sphincter iridis...article 1978
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- Gezondheidsorganisatie TNO Instituut voor experimentele gerontologie TNO (author), Kruisbeek, A.M. (author) doctoral thesis 1978
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Radiobiologisch Instituut TNO (author), Maat, B. (author)Recent data on extrapulmonary colony formation after heparin administration are inconclusive. A systemic study of this topic was undertaken with 4 experimental tumour systems and 2 distinct periods of reduced clotting capacity in rats and mice. I.v. injection of various numbers of tumour cells into i.p. heparinized animals leads to: Significant...article 1978
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Instituut voor experimentele gerontologie TNO (author), Blankwater, M.J. (author)A review of the present knowledge of the effect of age on the humoral immune response of mice is presented. It is shown that the capacity to mount an antibody response decreases with age, especially when the immune system is challenged for the first time by low doses of antigen, and when T cells are required for the response. Literature data are...article 1978
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Instituut voor experimentele gerontologie TNO (author), Knook, D.L. (author), Blansjaar, N. (author), Sleyster, E.C. (author)Non-parenchymal cell suspensions were prepared from rat livers by three different methods based on a collagenase, a pronase and a combined collagenase-pronase treatment. The highest yield of Kupffer and endothelial cells was obtained with the pronase treatment. Attempts were made for a further purification of these cells by Metrizamide density...article 1977
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Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)Highly purified suspensions of parenchymal, endothelial and Kupffer cells were prepared from the rat liver. The respective roles of these cell classes in the degradation of proteins was investigated by analysing the cellular distribution of two lysomal proteases. The specific arginine naphthylamidase activity was 2 times higher in Kupffer cells...article 1977
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Lubbe, F.H. (author), Rossi, G. (author), Zaalberg, O.B. (author), Medisch Biologisch Laboratorium TNO (author)A method is described for isolation of antibody forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37°C. During incubation rabbit anti mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were...article 1976