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Ghg balance of agricultural intensification & bioenergy production in the orinoquia region, colombiaRamírez-Contreras, N.E. (author), Munar-Florez, C. (author), van der Hilst, F. (author), Espinosa, J.C. (author), Ocampo-Duran, A. (author), Ruiz-Delgado, J. (author), Molina-Lopez, D.L. (author), Wicke, B. (author), Garcia-Nunez, J.A. (author), Faaij, A.P.C. (author)Energy crop expansion can increase land demand and generate displacement of food crops, which impacts greenhouse gas (GHG) emissions mainly through land-use change (LUC). Increased agricultural productivity could compensate for this. Our study aims to evaluate the regional combined GHG emissions of increasing agricultural yields for food crop...article 2021
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Zhao, Y. (author), Kumar, M. (author), Caspers, M.P.M. (author), Nierop Groot, M.N. (author), van der Vossen, J.M.B.M. (author), Abee, T. (author)Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate....article 2018
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Remington, B.C. (author), Westerhout, J. (author), Campbell, D.E. (author), Turner, P.J. (author)We analyzed reaction threshold data from 352 children undergoing open food challenges to hen's egg or cow's milk, either fresh or extensively heated into a muffin. There was no significant shift in dose-distribution curves due to the baking process, implying that existing threshold data for these allergens can be applied to allergen risk...article 2017
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Janus, M.M. (author), Keijser, B.J.F. (author), Bikker, F.J. (author), Exterkate, R.A.M. (author), Crielaard, W. (author), Krom, B.P. (author)Commensal oral biofilms, defined by the absence of pathology-related phenotypes, are ubiquitously present. In contrast to pathological biofilms commensal biofilms are rarely studied. Here, the effect of the initial inoculum and subsequent growth conditions on in vitro oral biofilms was studied. Biofilms were inoculated with saliva and grown...article 2015
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Ponstein-Simarro Doorten, A.Y. (author), van de Wiel, J.A.G. (author), Jonker, D. (author), TNO Kwaliteit van Leven (author)Tensguard™ is a milk protein hydrolysate containing the lactotripeptide IPP. It is derived from cow's milk, which is present in the human diet and has a safe history of consumption. The final Tensguard™ product, a supplement or a functional food ingredient, is intended for use by people who want to follow a healthy diet and lifestyle in order to...article 2009
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), Jansen, K.M.B. (author), Verhaar, J.A.N. (author), de Groot, J. (author), Vanosch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)To understand cartilage degenerative diseases and improve repair procedures, we investigate the influence of glycosaminoglycans (GAGs) on cartilage matrix biochemistry and functionality. Bovine articular chondrocytes were cultured in alginate beads with(out) para-nitrophenyl-beta-d-xyloside (PNPX) to inhibit GAG incorporation into newly formed...article 2009
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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), van der Linden, J.C. (author), Zuurmond, A.M. (author), Weinans, H. (author), Verhaar, J.A.N. (author), van Osch, G.J.V.M. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)Background: Damage to articular cartilage is one of the features of osteoarthritis (OA). Cartilage damage is characterised by a net loss of collagen and proteoglycans. The collagen network is considered highly important for cartilage function but little is known about processes that control composition and function of the cartilage collagen...article 2008
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Zerp, S.F. (author), Vink, S.R. (author), Ruiter, G.A. (author), Koolwijk, P. (author), Peters, E. (author), van der Luit, A.H. (author), de Jong, D. (author), Budde, M. (author), Bartelink, H. (author), van Blitterswijk, W.J. (author), Verheij, M. (author), TNO Kwaliteit van Leven (author)Synthetic alkylphospholipids (APLs), such as edelfosine, miltefosine and perifosine, constitute a new class of antineoplastic compounds with various clinical applications. Here we have evaluated the antiangiogenic properties of APLs. The sensitivity of three types of vascular endothelial cells (ECs) (bovine aortic ECs, human umbilical vein ECs...article 2008
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- Koek, M.M. (author), Muilwijk, B. (author), van Stee, L.L.P. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author) article 2008
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Ganzevles, R.A. (author), Fokkink, R. (author), van Vliet, T. (author), Cohen Stuart, M.A. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)Based on earlier reported surface rheological behaviour two factors appeared to be important for the functional behaviour of mixed protein/polysaccharide adsorbed layers at air/water interfaces: (1) protein/polysaccharide mixing ratio and (2) formation history of the layers. In this study complexes of β-lactoglobulin (positively charged at pH 4...article 2008
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TNO Kwaliteit van Leven (author), Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), Zuurmond, A.M. (author), Bank, R.A. (author), Verhaar, J.A.N. (author), de Groot, J. (author), van Osch, G.J.V.M. (author)Transforming growth factor beta (TGFbeta) is often used in cartilage tissue engineering to increase matrix formation by cells with various phenotypes. However, adverse effects of TGFbeta, such as extensive cross-linking in cultured fibroblasts, have also been reported. Our goal was to study effects of TGFbeta on collagen cross-linking and...article 2008
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Prado, M. (author), Berben, G. (author), Fumière, O. (author), van Duijn, G. (author), Mensinga-Kruize, J. (author), Reaney, S. (author), Boix, A. (author), von Holst, C. (author), TNO Kwaliteit van Leven (author)The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of...article 2007
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Halter, D. (author), Neumann, S. (author), van Dijk, S.M. (author), Wolthoorn, J. (author), de Mazière, A.M. (author), Vieira, O.V. (author), Mattjus, P. (author), Klumperman, J. (author), van Meer, G. (author), Sprong, H. (author), TNO Kwaliteit van Leven (author)Glycosphingolipids are controlled by the spatial organization of their metabolism and by transport specificity. Using immunoelectron microscopy, we localize to the Golgi stack the glycosyltransferases that produce glucosylceramide (GlcCer), lactosylceramide (LacCer), and GM3. GlcCer is synthesized on the cytosolic side and must translocate...article 2007
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Leeman, W.R. (author), van den Berg, K.J. (author), Houben, G.F. (author), TNO Kwaliteit van Leven (author)The human risk assessment of feed contaminants has often been hampered by a lack of knowledge concerning their behaviour when consumed by livestock. To gain a better understanding of the transfer of contaminants from animal feed to animal products, a meta-analysis of public literature was made. Data concerning feed contaminant concentrations,...article 2007
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Steenvoorden, M.M.C. (author), Bank, R.A. (author), Ronday, H.K. (author), Toes, R.E.M. (author), Huizinga, T.W.J. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)Objective: In vitro models for joint diseases often focus on a single cell type, such as chondrocytes in osteoarthritis (OA) or fibroblast-like synoviocytes (synoviocytes) in rheumatoid arthritis (RA). However, these joint diseases affect the whole joint and interaction between chondrocytes and synoviocytes may play an important role in disease...article 2007
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Eefting, D. (author), Grimbergen, J.M. (author), de Vries, M.R. (author), van Weel, V. (author), Kaijzel, E.L. (author), Que, I. (author), Moon, R.T. (author), Löwik, C.W. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)For the successful application of RNA interference in vivo, it is desired to achieve (local) delivery of small interfering RNAs (siRNAs) and long-term gene silencing. Nonviral electrodelivery is suitable to obtain local and prolonged expression of transgenes. By intramuscular electrodelivery of a plasmid in which two opposing human polymerase...article 2007
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Le Bizec, B. (author), Courant, F. (author), Gaudin, I. (author), Bichon, E. (author), Destrez, B. (author), Schilt, R. (author), Draisci, R. (author), Monteau, F. (author), André, F. (author), TNO Kwaliteit van Leven (author)Boldenone is an androgenic steroid that improves the growth and food conversion in food producing animals. In most countries worldwide, this anabolic steroid is forbidden for meat production. Until recently, the control of its illegal use was based either on 17β-boldenone or 17α-boldenone (its main metabolite in cattle) identification in edible...article 2006
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Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), Weinans, H. (author), Jahr, H. (author), Verhaar, J.A.N. (author), de Groot, J. (author), van Osch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)Objective: Examine effects of insulin-like growth factor 1 (IGF1), transforming growth factor β2 (TGFβ2) and fibroblast growth factor 2 (FGF2) on proteoglycan and collagen network and biomechanical properties of the newly formed cartilage matrix. Methods: Bovine articular chondrocytes were cultured in alginate beads for 3 weeks with or without...article 2006
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Valstar, D.L. (author), Schijf, M.A. (author), Arts, J.H.E. (author), Kuper, C.F. (author), Nijkamp, F.P. (author), Storm, G. (author), Bloksma, N. (author), Henricks, P.A.J. (author), TNO Kwaliteit van Leven (author)Occupational exposure to low molecular weight chemicals, like trimellitic anhydride (TMA), can result in occupational asthma. Alveolar macrophages (AMs) are among the first cells to encounter these inhaled compounds and were previously shown to affect TMA-induced asthma-like symptoms in the Brown Norway rat (Valstar et al., Toxicol. Appl....article 2006
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Appel, M.J. (author), van Veen, H.A. (author), Vietsch, H. (author), Salaheddine, M. (author), Nuijens, J.H. (author), Ziere, B. (author), de Loos, F. (author), TNO Kwaliteit van Leven (author)The oral toxicity of recombinant human lactoferrin (rhLF) produced in the milk of transgenic cows was investigated in Wistar rats by daily administration via oral gavage for 13 consecutive weeks, 7 days per week. The study used four groups of 20 rats/sex/dose. The control group received physiological saline and the three test groups received...article 2006