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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Hissink, A.M. (author), Oudshoorn, M.J. (author), van Ommen, B. (author), van Bladeren, P.J. (author)Our goal was to characterize possible species and strain differences in the hepatic microsomal biotransformation of 1,4-dichlorobenzene (1,4-DCB). Experiments compared extent of labeled 1,4-DCB conversion to oxidized metabolites, glutathione conjugates, and covalently bound metabolites by hepatic microsomes from humans, from male B6C3F1 mice,...article 1997
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Lina, B.A.R. (author), Bos-Kuijpers, M.H.M. (author), Til, H.P. (author), Bär, A. (author)The potential toxicity and carcinogenicity of erythritol, a low-calorie sugar substitute, were examined in Wistar Crl:(WI) WU BR rats. Groups of 50 rats of each sex consumed diets with 0, 2, 5, or 10% erythritol, or 10% mannitol, for a period of 104-107 weeks. To each of these main groups, two satellite groups of 20 males each were attached for...article 1996
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Visser, C.J.T. (author), van Garderen-Hoetmer, A. (author), Klijn, J.G.M. (author), Foekens, J.A. (author), Woutersen, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)In a previous short-term study (4 months) we found that Sandostatin, when administered prophylactically, inhibited growth of putative pre-neoplastic ductular lesions induced in hamster pancreas by N-nitrosobis(2-oxopropyl)amine (BOP), but not of acinar lesions induced in rat pancreas by azaserine. The present long-term (12 months) study was...article 1996
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Farmer, P.B. (author), Sepai, O. (author), Lawrence, R. (author), Autrup, H. (author), Nielsen, P.S. (author), Baan, R.A. (author), van Delft, J.H.M. (author), Steenwinkel, M.J.S.T. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)A coordinated study was carried out on the development, evaluation and application of biomonitoring procedures for populations exposed to environmental genotoxic pollutants. The procedures used involved both direct measurement of DNA or protein damage (adducts) and assessment of secondary biological effects (mutation and cytogenetic damage)....article 1996
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Jonker, D. (author), Woutersen, R.A. (author), Feron, V.J. (author), TNO Voeding (author)The toxicity of mixtures of chemicals with the same target organ was examined in rats using nephrotoxicants with similar or dissimilar modes of action. In a 4-wk feeding study, lysinoalanine, mercuric chloride, hexachloro-1,3-butadiene and d-limonene, each affecting renal proximal tubular cells but through different modes of action, were...article 1996
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Wormhoudt, L.W. (author), Ploemen, J.H.T.M. (author), de Waziers, I. (author), Commandeur, J.N.M. (author), Beaune, P.H. (author), van Bladeren, P.J. (author), Vermeulen, N.P.E. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)1,2-Dibromoethane (1,2-DBE) is mainly used as an additive in leaded gasoline and as a soil fumigant and it is a suspected carcinogen in humans. In this study, the oxidative bioactivation of 1,2-DBE to 2-bromoacetaldehyde (2-BA) was studied using heterologously expressed human cytochrome P450 (P450) isoenzymes and human liver microsomes. Out of...article 1996
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Tang, C. (author), Biemond, I. (author), Appel, M.J. (author), Visser, C.J.T. (author), Woutersen, R.A. (author), Lamers, C.B.H.W. (author), TNO Voeding (author)The growth of pancreatic cancers may be influenced by certain gut peptides. However, the alteration of gut peptide receptors in the progress of pancreatic carcinogenesis is largely unknown. With storage phosphor autoradiography, this study visualized and characterized receptors for cholecystokinin (CCK), somatostatin (SST), bombesin (BBS),...article 1996
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Groten, J.P. (author), Schoen, E.D. (author), Feron, V.J. (author), TNO Voeding (author)The use of factorial designs, in which n chemicals are studied at x(n) dose levels (x treatment groups), has been put forward as one of the valuable statistical approaches for hazard assessment of chemical mixtures. Very recently a '25 study' was presented to describe interactions between the carcinogenic activity of five polycyclic aromatic...article 1996
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- Hissink, E. (author), van Ommen, B. (author), Bogaards, J.J. (author), van Bladeren, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) article 1996
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Appel, M.J. (author), van Garderen-Hoetmer, A. (author), Woutersen, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)The effects of vitamins E and E, β-carotene and selenium on development of N-nitrosobis(2-oxopropyl)amine (BOP)-induced pancreatic tumours in hamsters were investigated. Dietary supplementation of vitamin C, alone as well as in combination with β-carotene resulted in consistently lower numbers of advanced ductular lesions. The differences with...article 1996
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Appel, M.J. (author), Woutersen, R.A. (author), TNO Voeding (author)In the present study the effects of 0.1 or 1.0 g β-carotene/kg diet (LβC or HβC) and 1.0 mg or 2.5 mg selenium/kg diet (LSel or HSel), as well as combinations of the respective low and high concentrations of p-carotene and selenium (LMix or HMix) on the initiation/early promotion phase or on the late promotion phase of pancreatic carcinogenesis...article 1996
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Noort, D. (author), Verheij, E.R. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO TNO Voeding (author)In this paper we describe the use of tandem mass spectrometry to identify modified sites in human hemoglobin after in vitro exposure to bis(2- chloroethyl) sulfide (sulfur mustard). Globin isolated from human whole blood which had been exposed to sulfur mustard was degraded with trypsin, and the digests were analyzed by micro LC/MS. Alkylated...article 1996
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Visser, C.J.T. (author), Bruggink, A.H. (author), Korc, M. (author), Kobrin, M.S. (author), de Weger, R.A. (author), Seifert-Bock, I. (author), van Blokland, W.T.M. (author), van Garderen Hoetmer, A. (author), Woutersen, R.A. (author), TNO Voeding (author)Using immunohistochemistry, Northern blotting and a semi-quantitative PCR technique, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and epidermal growth factor receptor (EGFR) expression were studied in the pancreas of N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. After initiation pancreatic carcinogenesis was...article 1996
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Instituut CIVO-Toxicologie en Voeding TNO (author), Appel, M.J. (author), Woutersen, R.A. (author)In the present study the putative chemopreventive effect of dietary fish oil (MaxEPA) on azaserine-induced pancreatic carcinogenesis in rats was investigated. Groups of rats were maintained on a semipurified low-fat (LF; 5 wt%) diet or on semipurified high-fat (HF; 25 wt%) diets containing 5 wt% linoleic acid (LA) and including 0.0, 1.2, 2.4, 4...article 1996
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Visser, C.J.T. (author), de Weger, R.A. (author), van Blokland, W.T.M. (author), Seifert-Bock, I. (author), Kobrin, M.S. (author), Korc, M. (author), Woutersen, R.A. (author)In the present study, the expression of the epidermal growth factor receptor (EGFR) was investigated in putative preneoplastic and neoplastic acinar cell lesions induced in the rat pancreas by azaserine, using Northern blotting, in situ hybridisation (ISH) and immunohistochemistry. EGFR protein levels were decreased in putative preneoplastic...article 1996
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Wolterbeek, A.P.M. (author), Roggeband, R. (author), Baan, R.A. (author), Feron, V.J. (author), Rutten, A.A.J.J.L. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Vitamin A and β-carotene protect against respiratory tract cancer by inhibiting the formation of DNA damage and controlling cellular proliferation and differentiation, Recently, it has been shown that the p53 tumor-suppressor gene plays a crucial role in the etiology of respiratory tract cancer. In the present study, we investigated the...article 1995
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Appel, M.J. (author), Woutersen, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)In the present study the chemopreventive potential of 25% fat (HF) diets containing 2 wt% linoleic acid (LA) and including 0.0, 1.2, 2.4, 4.7, 7.1 or 9.4 wt% dietary fish oil (MaxEPA) has been investigated using the N-nitrosobis(2-oxopropyl)amine (BOP)-hamster model for pancreatic cancer. The number of pancreatic borderline lesions (BLL) was...article 1995
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Steenwinkel, M.J.S.T. (author), Roggeband, R. (author), van Delft, J.H.M. (author), Baan, R.A. (author)This contribution describes methodological modifications and improvements that may contribute to inter-assay reproducibility and more accurate adduct quantification for 32P-postlabelling. Firstly, an anion-exchange chromatography procedure was developed to determine the amount of DNA used per assay and to check its purity, in particular to...bookPart 1993
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TNO Voeding (author), Meijers, M. (author), Appel, M.J. (author), van Garderen-Hoetmer, A. (author), Lamers, C.B.H.W. (author), Rovati, L.C. (author), Jansen, J.B.M.J. (author), Woutersen, R.A. (author)Cholecystokinin and bombesin have been shown to promote pancreatic growth and development of azaserine-induced acidophilic atypical acinar cell nodules in rat pancreas after treatment for 16 weeks. Lorglumide, a specific cholescystokinin receptor antagonist, inhibited the stimulating effect of cholecystokinin, but not of bombesin. The present...article 1992
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Instituut CIVO-Toxicologie en Voeding TNO (author), Verhagen, H. (author), Schilderman, P.A.E.L. (author), Kleinjans, J.C.S. (author)Butylated hydroxyanisole (BHA) is a synthetic food antioxidant used to prevent oils, fats and shortenings from oxidative deterioration and rancidity. This review depicts the current knowledge on BHA. The physical and chemical characteristics of BHA are summarized and its function as a food antioxidant is made clear. The toxicological...article 1991