Searched for: subject:"Bovinae"
(1 - 20 of 25)

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Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), Jansen, K.M.B. (author), Verhaar, J.A.N. (author), de Groot, J. (author), Vanosch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)
To understand cartilage degenerative diseases and improve repair procedures, we investigate the influence of glycosaminoglycans (GAGs) on cartilage matrix biochemistry and functionality. Bovine articular chondrocytes were cultured in alginate beads with(out) para-nitrophenyl-beta-d-xyloside (PNPX) to inhibit GAG incorporation into newly formed...
article 2009
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Prado, M. (author), Berben, G. (author), Fumière, O. (author), van Duijn, G. (author), Mensinga-Kruize, J. (author), Reaney, S. (author), Boix, A. (author), von Holst, C. (author), TNO Kwaliteit van Leven (author)
The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of...
article 2007
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Broersen, K. (author), Elshof, M. (author), de Groot, J. (author), Voragen, A.G.J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
A large number of proteins are glycosylated, either in vivo or as a result of industrial processing. Even though the effect of glycosylation on the aggregation of proteins has been studied extensively in the past, some reports show that the aggregation process is accelerated, whereas others found that the process is inhibited by glycosylation....
article 2007
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Eskes, C. (author), Bessou, S. (author), Bruner, L. (author), Curren, R. (author), Harbell, J. (author), Jones, P. (author), Kreiling, R. (author), Liebsch, M. (author), McNamee, P. (author), Pape, W. (author), Prinsen, M.K. (author), Seidle, T. (author), Vanparys, P. (author), Worth, A. (author), Zuang, V. (author), TNO Kwaliteit van Leven (author)
article 2005
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Carol, J. (author), Gorseling, M.C.J.K. (author), de Jong, C.F. (author), Lingeman, H. (author), Kientz, C.E. (author), van Baar, B.L.M. (author), Irth, H. (author), TNO Defensie en Veiligheid (author)
A multidimensional analytical method for the rapid determination and identification of proteins has been developed. The method is based on the size-exclusion fractionation of protein-containing samples, subsequent on-line trypsin digestion and desalination, and reversed-phase high-performance liquid chromatography-electrospray mass spectrometry...
article 2005
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Verwei, M. (author), van den Berg, H. (author), Havenaar, R. (author), Groten, J.P. (author), TNO Defensie en Veiligheid TNO Kwaliteit van Leven (author)
Background: Milk products are a potential matrix for fortification with synthetic folic acid or natural 5-methyltetrahydrofolate (5-CH 3-H4folate) to enhance the daily folate intake. In milk, folate occurs bound to folatebinding proteins (FBP). Our previous studies with an in vitro gastrointestinal model showed that 70% of the initial FBP...
article 2005
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de Brabander, H.F. (author), Poelmans, S. (author), Schilt, R. (author), Stephany, R.W. (author), le Bizec, B. (author), Draisci, R. (author), Sterk, S.S. (author), van Ginkel, L.A. (author), Courtheyn, D. (author), van Hoof, N. (author), Macrì, A. (author), de Wasch, K. (author), TNO Voeding (author)
The review summarizes current knowledge on the possible illegal use of the anabolic steroid boldenone. The presence of boldenone and metabolites in different animal species and the possibility of the occurrence of endogenous boldenone and metabolites is assessed, as are the methods of analysis used for detection. Different laboratories in the...
article 2004
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Nieuwenhuizen, W.F. (author), Dekker, H.L. (author), Gröneveld, T. (author), de Koster, C.G. (author), de Jong, G.A.H. (author), TNO Voeding (author)
Bovine β-lactoglobulin (BLG) is a major component in whey and its physical properties are important for the texture of many dairy-based foods. Modification of proteins with transglutaminase from Streptoverticillium mobaraense (MTGase) can be used to alter their physical properties. MTGase-mediated modification of native BLG was until now,...
article 2004
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Valencia, J.V. (author), Weldon, S.C. (author), Quinn, D. (author), Kiers, G.H. (author), de Groot, J. (author), TeKoppele, J.M. (author), Hughes, T.E. (author), Gaubius instituut TNO (author)
Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for advanced glycation end products (RAGE). The dependence of RAGE binding on specific biochemical characteristics of AGEs is currently unknown. Using standardized procedures and a variety of...
article 2004
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Nieuwenhuizen, W.F. (author), Dekker, H.L. (author), de Koning, L.J. (author), Gröneveld, T. (author), de Koster, C.G. (author), de Jong, G.A.H. (author)
The molecular structures determine the physical properties of milk proteins and are important for the texture of many dairy-based foods. Bovine α-lactalbumin (α-LA) is a globular 123 amino acid Ca2+ binding milk protein. Modification with microbial Ca2+ independent transglutaminase (MTGase) was used to modify lysines and glutamines in holo and...
article 2003
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de Jong, G.A.H. (author), Wijngaards, G. (author), Koppelman, S.J. (author), TNO Voeding (author)
Cross-linking experiments of skimmed bovine milk with bacterial transglutaminase isolated from Streptoverticillium mobaraense showed only some degree of formation of high-molecular-weight casein polymers. Studies on the nature of this phenomenon revealed that bovine milk contains an inhibitor of transglutaminase activity. Removal of the casein...
article 2003
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Prigent, S.V.E. (author), Gruppen, H. (author), Visser, A.J.W.G. (author), van Koningsveld, G.A. (author), de Jong, G.A.H. (author), Voragen, A.G.J. (author)
The non-covalent interactions between the monomeric phenolic compound chlorogenic acid (5-CQA) and bovine serum albumin (BSA), lysozyme, and α-lactalbumin were characterized, and their effect on protein properties was examined. 5-CQA had a low affinity for all three proteins, and these interactions seemed to show a negative cooperativity. 5-CQA...
article 2003
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de Jong, G.A.H. (author), Wijngaards, G. (author), Boumans, H. (author), Koppelman, S. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A procedure for a fast and simple purification of bovine plasma transglutaminase was developed, which resulted in a homogeneous enzyme preparation. Two different procedures were developed for the purification of pig erythrocyte transglutaminase, both of which resulted in partial purification. Both enzymes were used in cross-linking reactions of...
article 2001
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Lamfers, M.L.M. (author), Lardenoye, J.H.P. (author), de Vries, M.R. (author), Aalders, M.C. (author), Engelse, M.A. (author), Grimbergen, J.M. (author), van Hinsbergh, V.W.M. (author), Quax, P.H.A. (author), Gaubius Instituut TNO (author)
Injury-induced neointimal development results from migration and proliferation of vascular smooth muscle cells (SMC). Cell migration requires controlled proteolytic degradation of extracellular matrix surrounding the cell. Plasmin is a major contributor to this process by degrading various matrix proteins directly, or indirectly by activating...
article 2001
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de Groot, J. (author), Verzijl, N. (author), Budde, M. (author), Bijlsma, J.W.J. (author), Lafeber, F.P.J.G. (author), TeKoppele, J.M. (author), Gaubius Instituut (author)
The integrity of the collagen network is essential for articular cartilage to fulfill its function in load support and distribution. Damage to the collagen network is one of the first characteristics of osteoarthritis. Since extensive collagen damage is considered irreversible, it is crucial that chondrocytes maintain a functional collagen...
article 2001
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Graaf, I.A.M. (author), Tajima, O. (author), Groten, J.P. (author), Wolterbeek, A.P.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2,2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI...
article 2000
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van Beek, E. (author), Pieterman, E. (author), Cohen, L. (author), Löwik, C. (author), Papapoulos, S. (author), Gaubius instituut TNO (author)
Bisphosphonates (Bps), inhibitors of osteoclastic bone resorption, are used in the treatment of skeletal disorders. Recent evidence indicated that farnesyl pyrophosphate (FPP) synthase and/or isopentenyl pyrophosphate (IPP) isomerase is the intracellular target(s) of bisphosphonate action. To examine which enzyme is specifically affected, we...
article 1999
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van Noort, J.M. (author), van Veelen, P. (author), Hopstaken, F. (author), TNO Preventie en Gezondheid (author)
The stress protein α B-crystallin was recently identified as a component of central nervous system myelin that is strongly immunogenic to human T cells. Stress-induced αB-crystallin that accumulates in the central nervous system is phosphorylated and recent evidence indicates that both rodent and human T cells can discriminate between...
article 1998
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Gaubius instituut TNO (author), Hendriks, W.L. (author), van Vark, L.C. (author), Schoonderwoerd, K. (author), Jansen, H. (author), Havekes, L.M. (author)
Lipoprotein lipase (LPL) purified from bovine milk showed variable abilities to stimulate the binding of low density lipoprotein (LDL) to J774 macrophages. The presence of a 37 kDa protein in the LPL sample seemed to be of importance for its stimulatory capacity. In order to investigate this, we isolated LPL from bovine milk via heparin...
article 1998
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Beekman, B. (author), Verzijl, N. (author), Bank, R.A. (author), Von Der Mark, K. (author), TeKoppele, J.M. (author), TNO Preventie en Gezondheid (author)
The extracellular matrix synthesized by articular chondrocytes cultured in alginate beads was investigated. Collagen levels increased sigmoidally with time and remained constant after 2 weeks of culture. The presence of cartilage-specific type II collagen was confirmed immunohistochemically. Predominantly type H collagen was present in the...
article 1997
Searched for: subject:"Bovinae"
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