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Effects of moderate alcohol consumption on platelet aggregation, fibrinolysis, and blood lipids

Pikaar, N.A. (author), Wedel, M. (author), van der Beek, E.J. (author), van Dokkum, W. (author), Kempen, H.J.M. (author), Kluft, C. (author), Ockhuizen, T. (author), Hermus, R.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

To investigate the effect of moderate alcohol consumption on blood constituents related to cardiovascular disease, 12 male volunteers consumed (instead of their usual alcoholic drinks) four different standardized amounts of red wine in addition to their habitual diet. Each dose was given to the subjects during a period of 5 weeks in a randomized...

article 1987

document

Plasminogen activator inhibitors

Sprengers, E.D. (author), Kluft, C. (author), Gaubius Instituut TNO (author)

Chemicals/CAS: fibrin, 9001-31-4; plasminogen activator inhibitor, 105844-41-5; plasminogen activator, 9039-53-6; Carrier Proteins; Glycoproteins; Plasminogen Activators, EC 3.4.21.-; Plasminogen Inactivators; Protease Inhibitors; protease-nexin

article 1987

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The 5T2 mouse multiple myeloma model: Characterization of 5T2 cells within the bone marrow

Instituut voor Experimentele Gerontologie TNO (author), Croese, J.W. (author), Vas Nunes, C.M. (author), Radl, J. (author), Enden-Vieveen, M.H.M. (author), van den Brondijk, R.J. (author), Boersma, W.J.A. (author)

The transplantable C57BL/KaLwRij mouse 5T2 multiple myeloma (MM) is a new animal model for studies on MM in man. Histological examination of the 5T2 MM cells revealed their morphological heterogeneity. In this study we investigated whether this heterogeneity reflects subpopulations of 5T2 MM cells with different biological properties. 5T2 MM...

article 1987

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A rapid micromethod for apolipoprotein E phenotyping directly in serum

Gaubius instituut TNO (author), Havekes, L.M. (author), de Knijff, P. (author), Beisiegel, U. (author), Havinga, J. (author), Smit, M. (author), Klasen, E. (author)

A new method for the apolipoprotein E phenotyping has been developed. The method is based on isoelectric focusing of either delipidated or guanidine-HCl-treated serum or plasma in a horizontal slab gel system followed by immunoblotting using either polyclonal or monoclonal anti-apolipoprotein E antibodies as first antibody. Apolipoprotein E...

article 1987

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The cofactor role of protein S in the acceleration of whole blood clot lysis by activated protein C in vitro

de Fouw, N.J. (author), Haverkate, F. (author), Bertina, R.M. (author), Gaubius Instituut TNO (author)

The effect of purified human activated protein C (APC) and protein S on fibrinolysis was studied by using an in vitro blood clot lysis technique. Blood clots were formed from citrated blood (supplemented with 125I-fibrinogen) by adding thrombin and Ca2+-ions; lysis of the clots was achieved by adding tissue-type plasminogen activator. The...

article 1986

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Assignment of the human tissue-type plasminogen activator gene (PLAT) to chromosome 8

Verheijen, J.H. (author), Visse, R. (author), Wijnen, T.J. (author), Gaubius Instituut TNO (author)

Using 1.2kb 3'-terminal Pst-I fragment of a full length tissue-type plasminogen activator (t-PA) cDNA clone (ptPA-8FL) and a set of rodent human somatic cell hydrids, the corresponding human gene PLAT was localized on chromosome 8. Chemicals/CAS: plasminogen activator, 9039-53-6; DNA Restriction Enzymes, EC 3.1.21; DNA, 9007-49-2; Genetic...

article 1986

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The mutual relationship between the two molecular forms of the major fibrinolysis inhibitor alpha-2-antiplasmin in blood

Kluft, C. (author), Los, P. (author), Jie, A.F.H. (author), Gaubius instituut TNO (author)

Alpha-2-antiplasmin, a major inhibitor of fibrinolysis, is synthesized in the liver and occurs in blood in two molecular forms: a very active plasminogen-binding (PB) form and a less active nonplasminogen-binding (NPB) form. This study investigated the origin and mutual relationship of these two forms in vivo and in vitro. Despite wide variation...

article 1986

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Excretion-retention data of steady state gas exchange in tidal breathing. I.Dependency on the blood-gas partition coefficient

Zwart, A. (author), Luijendijk, S.C.M. (author), de Vries, W.R. (author), Instituut CIVO-Toxicologie en voeding TNO (author)

article 1986

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Microdetermination of vitamin A in human plasma using high-performance liquid chromatography with fluorescence detection

Speek, A.J. (author), Wongkham, C. (author), Limratana, N. (author), Saowakontha, S. (author), Schreurs, W.H.P. (author), Instituut CIVO-Toxicologie en voeding TNO (author)

Chemicals/CAS: n,n dimethylacetamide, 127-19-5; retinol, 68-26-8, 82445-97-4; Vitamin A, 11103-57-4

article 1986

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Hemostasis and fibrinolysis in severe liver failure and their relation to hemorrhage

Gaubius instituut TNO (author), Boks, A.L. (author), Brommer, E.J.P. (author), Schalm, S.W. (author), van Vliet, H.H.D.M. (author)

In a study of severe, decompensated liver failure, we tried to find a correlation between hemorrhage and parameters of hemostasis and fibrinolysis. Three groups of patients were studied: alcoholic cirrhosis; nonalcoholic cirrhosis, and acute liver failure without known prior liver disease. The two cirrhotic groups did not differ significantly...

article 1986

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Production and characterization of a monoclonal antibody reactive with a specific neoantigenic determinant (comprising B?? 54-118) in degradation products of fibrin and of fibrinogen

Koppert, P.W. (author), Koopman, J. (author), Haverkate, F. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)

Balb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human bloot clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that produces monoclonal...

article 1986

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Effect of work load on free and sulfate-conjugated plasma catecholamines, prolactin, and cortisol

Instituut CIVO-Toxicologie en voeding TNO (author), Odink, J. (author), van der Beek, E.J. (author), van den Berg, H. (author), Bogaards, J.J.P. (author), Thissen, J.T.N.M. (author)

The effects of maximal performance capacity and different work load intensities on the response of free and sulfate-conjugated plasma catecholamines, prolactin (PRL), and cortisol were studied in six healthy male volunteers. In a first experiment, the volunteers had to perform a step-wise incremental bicycle ergometer test to determine the onset...

article 1986

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Excretion-retention data of steady state gas exchange in tidal breathing. II. Dependency on the diffusion coefficient

Centraal Instituut voor Voedingsonderzoek TNO (author), Zwart, A. (author), Luijendijk, S.C.M. (author), de Vries, W.R. (author)

article 1986

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Determination of fibrin(ogen) degradation products (FDP) in plasma, using a monoclonal antibody

Haverkate, F. (author), Koopman, J. (author), Koppert, P. (author), Nieuwenhuizen, W. (author), Gaubius Institute, Health Research Division TNO, 2313 AD Leiden, Netherlands (author)

article 1985

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Fibrinolytic shut-down after surgery: Impairment of the balance between tissue-type plasminogen activator and its specific inhibitor

D'Angelo, A. (author), Kluft, C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

In nine patients with non-malignant diseases undergoing major upper abdominal surgery, the mechanism of the postoperative fibrinolytic shut-down was investigated because of its potential significance for postoperative deep vein thrombosis by employing new and specific methods for assessing and stimulating the fibrinolytic system. The shut-down...

article 1985

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The influence of radiotherapy and chemotherapy on the vitamin status of cancer patients

Schreurs, W.H.P. (author), Odink, J. (author), Egger, R.J. (author), Wedel, M. (author), Bruning, P.F. (author), Instituut CIVO-Toxicologie en voeding TNO (author)

Chemicals/CAS: Ascorbic Acid, 50-81-7; Calcifediol, 19356-17-3; Vitamin A, 11103-57-4; Vitamin B 12, 68-19-9; Vitamin E, 1406-18-4; Vitamins

article 1985

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A monoclonal antibody, specific for human fibrinogen, fibrinopeptide A-containing fragments and not reacting with free fibrinopeptide A

Koppert, P.W. (author), Huijsmans, C.M.G. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)

Spleen cells of BALB/c mice, immunized with fragments Y of normal human fibrinogen, were fused with P3 x 63 Ag 8653 myeloma cells. A clone was found which produces monoclonal antibodies (Mab-Y18) of the IgM?? type. Mab-Y18 is immunoreactive with normal human fibrinogen, and its fragments X, Y, N-terminal disulphide knot, A??-chain, and A??...

article 1985

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Effects of feeding stannous chloride on different parts of the gastrointestinal tract of the rat

Janssen, P.J.M. (author), Bosland, M.C. (author), van Hees, J.P. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)

The effects of feeding inorganic tin on the gastrointestinal tract were examined in rats. Three groups of male weanling Wistar rats were fed a diet to which 0, 250, or 500 ppm Sn2+ had been added as SnCl2. A fourth group was subjected to feed restriction by pair feeding with the 500-ppm group. Comparison of the data from the tin-fed groups with...

article 1985

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Activated protein C decreases plasminogen activator-inhibitor activity in endothelial cell-conditioned medium

van Hinsbergh, V.W.M. (author), Bertina, R.M. (author), van Wijngaarden, A. (author), van Tilburg, N.H. (author), Emeis, J.J. (author), Haverkate, F. (author), Gaubius instituut TNO (author)

Confluent cultures of endothelial cells from human umbilical cord were used to study the effect of activated human protein C (APC) on the production of plasminogen activators, plasminogen activator-inhibitor, and factor VIII-related antigen. Addition of APC to the cells in a serum-free medium did not affect the production of tissue-type...

article 1985

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PAF-acether-induced release of tissue-type plasminogen activator from vessel walls

Emeis, J.J. (author), Kluft, C. (author), Gaubius instituut TNO (author)

Platelet-activating factor (PAF-acether; 1-O-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF...

article 1985