Sub-chronic (13-week) oral toxicity study in rats with recombinant human lactoferrin produced in the milk of transgenic cows

The oral toxicity of recombinant human lactoferrin (rhLF) produced in the milk of transgenic cows was investigated in Wistar rats by daily administration via oral gavage for 13 consecutive weeks, 7 days per week. The study used four groups of 20 rats/sex/dose. The control group received physiological saline and the three test groups received...

Evaluation of the toxicity of concentrated barley β-glucan in a 28-day feeding study in Wistar rats

β-Glucans are water-soluble cell-wall polysaccharides consisting of (1→3,1→4)-linked β-D-glucopyranosyl monomers that comprise a considerable proportion of soluble fiber from certain grains including oats and barley. Consumption of foods containing β-glucan or β-glucan-enriched fractions prepared from these grains lower serum cholesterol...

Effect of nondigestible oligosaccharides on large-bowel functions, blood lipid concentrations and glucose absorption in young healthy male subjects

Objective: To study the effect of the intake of 15 g nondigestible oligosaccharides per day on various parameters of large-bowel function, as well as on blood lipid concentrations and glucose absorption in man. Design: Latin square, randomized, double-blind, diet-controlled. Setting: Metabolic research unit. Subjects: Twelve apparently healthy...

Pathological and immunological effects of respirable coal fly ash in male Wistar rats

In this study the effects of inhalatory exposure to coal fly ash on lung pathology and the immune system in rats were examined. Rats were exposed to 0, 10, 30, or 100 mg/m3 coal fly ash (6 h/day, 5 days/wk) for 4 wk, or to 0 and 100 mg/m3 for 1 wk, and for 1 wk followed by a recovery in clean air of 3 wk. A concentration-related increase in lung...

Subacute toxicity of a mixture of nine chemicals in rats: Detecting interactive effects with a fractionated two-level factorial design

The present study was intended (1) to find out whether simultaneous administration of nine chemicals at a concentration equal to the 'no-observed-adverse-effect level' (NOAEL) for each of them would result in a NOAEL for the combination and (2) to test the usefulness of fractionated factorial models to detect possible interactions between...

Effect of coexposure to methyl ethyl ketone (MEK) on n-hexane toxicokinetics in human volunteers

In order to study the effects of methyl ethyl ketone (MEK) on the toxicokinetics of n-hexane and, in particular, the formation of 2,5-hexanedione from n-hexane in humans, volunteers were exposed to n-hexane (approx. 60 ppm, 2.4 μM in the inhaled air) with or without simultaneous inhalatory coexposure to MEK for 15.5 min. The concentration-time...

Human diets cooked by microwave or conventionally: Comparative sub-chronic (13-wk) toxicity study in rats

To compare the possible effects of microwave and conventional cooking on a range of common dietary components, mixed human diets containing beef, potatoes and vegetables were fed to groups of 10 male and 10 female Wistar rats for 13 wk. The diet ingredients were cooked by either of the methods in a normal and an abused manner, the latter...