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- Gaubius Instituut TNO (author), van der Laan, W.H. (author), Ronday, H.K. (author), Grimbergen, J.M. (author), Huisman, L.G.M. (author), Breedveld, F.C. (author), te Koppele, J.M. (author), Huizinga, T.W.J. (author), Quax, P.H.A. (author), Verheijen, J.H. (author) article 1999
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- Gaubius Instituut TNO (author), Kluft, C. (author), Meijer, P. (author), Gram, J. (author), Jespersen, J. (author), Sidelmann, J. (author), te Koppele, J.M. (author), Buytenhek, M. (author) conference paper 1999
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Gaubius instituut TNO (author), Bank, R.A. (author), Tekoppele, J.M. (author), Oostingh, G. (author), Hazleman, B.L. (author), Riley, G.P. (author)Objectives - To investigate age related and site specific variations in turnover and chemistry of the collagen network in healthy tendons as well as the role of collagen remodelling in the degeneration of the supraspinatus tendon (ST-D) in rotator cuff tendinitis. Methods - Collagen content and the amount of hydroxylysine (Hyl),...article 1999
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Ronday, H.K. (author), TeKoppele, J.M. (author), Greenwald, R.A. (author), Moak, S.A. (author), de Roos, J.A.D.M. (author), Dijkmans, B.A.C. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)The plasminogen activation system is one of the enzyme systems held responsible for bone and cartilage degradation in rheumatoid arthritis (RA). In this study, we evaluated the effect of tranexamic acid (TEA), an inhibitor of plasminogen activation, on urinary collagen cross-link excretion and radiological joint damage in rat adjuvant arthritis ...article 1998
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Bank, R.A. (author), Bayliss, M.T. (author), Lafeber, F.P.J.G. (author), Maroudas, A. (author), Tekoppele, J.M. (author), Gaubius Instituut TNO (author)A biomechanical failure of the collagen network is postulated in many hypotheses of the development of osteoarthritis with advancing age. Here we investigate the accumulation of non-enzymatic glycation (NEG) products in healthy human articular cartilage, its relation to tissue remodelling and its role in tissue stiffening. Pentosidine levels...article 1998
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TeKoppele, J.M. (author), Beekman, B. (author), Verzijl, N. (author), Koopman, J.L. (author), de Groot, J. (author), Bank, R.A. (author), Gaubius Instituut TNO (author)Doxycycline (DOX) profoundly inhibited collagen synthesis by differentiated articular chondrocytes. At 25 microM, the rate of collagen synthesis was suppressed by more than 50% without affecting cell proliferation (DNA levels) and general protein synthesis (35S-Met and 35S-Cys incorporation). Steady-state mRNA levels of type II collagen were...article 1998
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Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), TeKoppele, J.M. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)Objective: To determine the role of interleukin-1β (IL-1β) in the degradation of proteoglycans and collagen by articular chondrocytes. Design: Chondrocytes were cultured in alginate beads for 2 weeks to produce extracellular matrix, followed by the addition of IL-1β for 1 or 2 days. Breakdown of extracellular matrix (with and without activation...article 1998
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Smulders, R.A. (author), Stehouwer, C.D.A. (author), Schalkwijk, C.G. (author), Donker, A.J.M. (author), van Hinsbergh, V.W.M. (author), TeKoppele, J.M. (author), TNO Preventie en Gezondheid (author)Dysfunction of the vascular endothelium is considered an early step in the development of diabetic angiopathy. Hyperglycaemia results in endothelial dysfunction, both through direct effects of glucose and through formation of advanced glycosylation end-products (AGEs). We hypothesized that the effects of glucose and AGEs on endothelial function...article 1998
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- Gaubius Instituut TNO (author), Veraart, J.R. (author), Kok, S.J. (author), TeKoppele, J.M. (author), Gooijer, C. (author), Lingeman, H. (author), Velthorst, N.H. (author), Brinkman, U.A.Th. (author) article 1998
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Gaubius Instituut TNO (author), Brama, P.A.J. (author), TeKoppele, J.M. (author), Beekman, B. (author), van Weeren, P.R. (author), Barneveld, A. (author)Objective - To investigate the influence of age, osteoarthritis (OA), and osteochondrosis (OC) on the matrix metalloproteinase (MMP) activity in the synovial fluid (SF) of equine joints. Methods - SF was collected from normal and osteoarthritic metacarpophalangeal joints (normal: 14 adult, 28 juvenile; OA: 22 adult). And from normal and...article 1998
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- Bank, R.A. (author), Krikken, M. (author), Beekman, B. (author), Stoop, R. (author), Maroudas, A. (author), Lafeber, F.P.J.G. (author), TeKoppele, J.M. (author) article 1997
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- van Valburg, A.A. (author), Wenting, M.J.G. (author), Beekman, B. (author), TeKoppele, J.M. (author), Lafeber, F.P.G.J. (author), Bijlsma, J.W.J. (author) article 1997
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Beekman, B. (author), Verzijl, N. (author), Bank, R.A. (author), Von Der Mark, K. (author), TeKoppele, J.M. (author), TNO Preventie en Gezondheid (author)The extracellular matrix synthesized by articular chondrocytes cultured in alginate beads was investigated. Collagen levels increased sigmoidally with time and remained constant after 2 weeks of culture. The presence of cartilage-specific type II collagen was confirmed immunohistochemically. Predominantly type H collagen was present in the...article 1997
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Bank, R.A. (author), Beekman, B. (author), Tenni, R. (author), Tekoppele, J.M. (author), Gaubius Instituut TNO (author)Measurement of the glycosylated hydroxylysines galactosyl- and glucosylgalactosylhydroxylysine (GH and GGH) in combination with other amino acids has been based on ion-exchange chromatography followed by reaction with ninhydrin. Here, a rapid and sensitive high-performance liquid chromatographic method with fluorimetric detection has been...article 1997
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Bank, R.A. (author), Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), Nico Sakkee, A. (author), Tekoppele, J.M. (author), TNO Preventie en Gezondheid (author)A high-performance liquid chromatographic assay was developed for pyridinium crosslinks and pentosidine in mature collagen of a wide variety of connective tissue hydrolysates by a simple two-step isocratic assay using a reversed-phase column. The crosslinks (including the internal standard pyridoxine) were optimally detected by their native...article 1997
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Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), Koopman, J.L. (author), Tekoppele, J.M. (author), Leiden, Netherlands Gaubius Instituut TNO (author)Doxycycline is known for its ability to inhibit matrix metalloproteinases (MMPs), a family of enzymes that play a role in cartilage breakdown in arthritides. Its prophylactic effect in reducing joint degradation in osteoarthritis is mainly attributed to this property. In this study, we show that doxycycline exhibits a profound inhibition of...article 1997
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Gaubius Instituut TNO (author), Beekman, B. (author), van El, B. (author), Drijfhout, J.W. (author), Ronday, H.K. (author), TeKoppele, J.M. (author)Stromelysin-1 (MMP-3) is an important member of the matrix metalloproteinase family. In joint-degrading diseases like arthritis, elevated levels of MMP-3 protein are detected in synovial fluid using immunological methods. However, these methods do not discriminate between active and inactive enzyme. In the present study, a specific stromelysin...article 1997
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Meulenbelt, I. (author), Bijkerk, C. (author), de Wildt, S.C.M. (author), Miedema, H.S. (author), Valkenburg, H.A. (author), Breedveld, F.C. (author), Pols, H.A.P. (author), TeKoppele, J.M. (author), Sloos, V.F.G. (author), Hofman, A. (author), Slagboom, P.E. (author), van Duijn, C.M. (author)Objective. To investigate whether radiographically evident osteoarthritis (ROA) in 55-65-year-old men and women is associated with specific alleles or genotypes of the cartilage matrix protein (CRTM) and cartilage link protein (CRTL1) genes. Methods. Cases were selected from a population-based study on the presence of ROA of the knee or hip....article 1997
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- Bank, R.A. (author), Jansen, E.J. (author), Beekman, B. (author), te Koppele, J.M. (author), Gaubius Instituut TNO (author) article 1996
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- te Koppele, J.M. (author), Lucassen, P.J. (author), Sakkee, A.N. (author), van Asten, J.G. (author), Ravid, R. (author), Swaab, D.F. (author), van Bezooijen, C.F.A. (author), Gaubius Instituut TNO (author) article 1996