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Beulens, J.W.J. (author), Rimm, E.B. (author), Hu, F.B. (author), Hendriks, H.F.J. (author), Mukamal, K.J. (author), TNO Kwaliteit van Leven (author)OBJECTIVE - The purpose of this study was to investigate whether adiponectin concentrations and biomarkers of inflammation, endothelial dysfunction, and insulin resistance mediate the association between alcohol consumption and diabetes. RESEARCH DESIGN AND METHODS - In a nested case-control study of 705 women with incident diabetes and 787...article 2008
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de Lau, L.M.L. (author), Cheung, E.Y.L. (author), Kluft, C. (author), Leebeek, F.W.G. (author), Meijer, P. (author), Laterveer, R. (author), Dippel, D.W.J. (author), de Maat, M.P.M. (author), TNO Kwaliteit van Leven (author)Ischemic stroke is associated with leucocyte activation. Activated leucocytes release elastase, an enzyme that can degrade fibrinogen. Fibrinogen elastase degradation products (FgEDP) may serve as a specific marker of elastase proteolytic activity. In a case-control study of 111 ischemic stroke patients and 119 controls, significantly higher...article 2008
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Hemelaar, M. (author), van der Mooren, M.J. (author), Rad, M. (author), Kluft, C. (author), Kenemans, P. (author), TNO Kwaliteit van Leven (author)Objective: To review the effects of non-oral administration of postmenopausal hormone therapy (HT) on risk markers for atherosclerotic and venous thromboembolic disease.Non-oral postmenopausal HT appears not to increase venous thromboembolic risk, whereas the effect on coronary heart disease risk is less clear. Design: Systematic review of...article 2008
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Berbée, J.F.P. (author), van der Hoogt, C.C. (author), de Haas, C.J.C. (author), van Kessel, K.P.M. (author), Dallinga-Thie, G.M. (author), Romijn, J.A. (author), Havekes, L.M. (author), van Leeuwen, H.J. (author), Rensen, P.C.N. (author), TNO Kwaliteit van Leven (author)Objective: We recently reported that apolipoprotein CI (apoCI) protects against the development of murine bacterial sepsis. We now examined the time course of plasma apoCI levels in survivors and non-survivors of severe sepsis. Design: Prospective study in patients meeting predefined criteria for severe sepsis. Setting: University hospital...article 2008
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TNO Kwaliteit van Leven (author), Adourian, A. (author), Jennings, E. (author), Balasubramanian, R. (author), Hines, W.M. (author), Damian, D. (author), Plasterer, T.N. (author), Clish, C.B. (author), Stroobant, P. (author), McBurney, R. (author), Verheij, E.R. (author), Bobeldijk, I. (author), van der Greef, J. (author), Lindberg, J. (author), Kenne, K. (author), Andersson, U. (author), Hellmold, H. (author), Nilsson, K. (author), Salter, H. (author), Schuppe-Koistinen, I. (author)High-throughput biomolecular profiling techniques such as transcriptomics, proteomics and metabolomics are increasingly being used in in vivo studies to recognize and characterize effects of xenobiotics on organs and systems. Of particular interest are biomarkers of treatment-related effects which are detectible in easily accessible biological...article 2008
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Transcriptional activity around bacterial cell death reveals molecular biomarkers for cell viabilityTNO Kwaliteit van Leven (author), Kort, R. (author), Keijser, B.J. (author), Caspers, M.P.M. (author), Schuren, F.H. (author), Montijn, R. (author)Background: In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias...article 2008
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TNO Kwaliteit van Leven (author), van Ommen, B. (author), Fairweather-Tait, S. (author), Freidig, A. (author), Kardinaal, A. (author), Scalbert, A. (author), Wopereis, S. (author)Micronutrients are involved in specific biochemical pathways and have dedicated functions in the body, but they are also interconnected in complex metabolic networks, such as oxidative-reductive and inflammatory pathways and hormonal regulation, in which the overarching function is to optimise health. Post-genomic technologies, in particular...article 2008
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TNO Kwaliteit van Leven (author), Hunter, D.J. (author), LaValley, M. (author), Li, J. (author), Bauer, D.C. (author), Nevitt, M. (author), de Groot, J. (author), Poole, R. (author), Eyre, D. (author), Guermazi, A. (author), Gale, D. (author), Totterman, S. (author), Felson, D.T. (author)Introduction: Our objective was to determine whether markers of bone resorption and formation could serve as markers for the presence of bone marrow lesions (BMLs). Methods: We conducted an analysis of data from the Boston Osteoarthritis of the Knee Study (BOKS). Knee magnetic resonance images were scored for BMLs using a semiquantitative...article 2008
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TNO Kwaliteit van Leven (author), van Ommen, B. (author), Keijer, J. (author), Kleemann, R. (author), Elliott, R. (author), Drevon, C.A. (author), McArdle, H. (author), Gibney, M. (author), Müller, M. (author)In quantifying the beneficial effect of dietary interventions in healthy subjects, nutrition research meets a number of new challenges. Inter individual variation in biomarker values often is larger than the effect related to the intervention. Healthy subjects have a remarkable capacity to maintain homeostasis, both through direct metabolic...article 2008
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Tchetverikov, I. (author), Kraan, M.C. (author), van El, B. (author), Hanemaaijer, R. (author), de Groot, J. (author), Huizinga, T.W.J. (author)Objective: To analyse the effects of leflunomide and methotrexate treatment on matrix metalloproteinase (MMP) activity levels in a2 macroglobulin/MMP (α2M/MMP) complexes in the systemic circulation of rheumatoid arthritis (RA) patients. Methods: A total of 102 RA patients from a prospective, double-blind, randomised clinical trial comparing...article 2008
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van Weel, V. (author), Toes, R.E.M. (author), Seghers, L. (author), Deckers, M.M.L. (author), de Vries, M.R. (author), Eilers, P.H. (author), Sipkens, J. (author), Schepers, A. (author), Eefting, D. (author), van Hinsbergh, V.W.M. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)OBJECTIVE - The immune system is thought to play a crucial role in regulating collateral circulation (arteriogenesis), a vital compensatory mechanism in patients with arterial obstructive disease. Here, we studied the role of lymphocytes in a murine model of hindlimb ischemia. METHODS AND RESULTS - Lymphocytes, detected with markers for NK1.1,...article 2007
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Hendriks, M.M.W.B. (author), Smit, S. (author), Akkermans, W.L.M.W. (author), Reijmers, T.H. (author), Eilers, P.H.C. (author), Hoefsloot, H.C.J. (author), Rubingh, C.M. (author), de Koster, C.G. (author), Aerts, J.M. (author), Smilde, A.K. (author), TNO Kwaliteit van Leven (author)SELDI-TOF-MS is rapidly gaining popularity as a screening tool for clinical applications of proteomics. Application of adequate statistical techniques in all the stages from measurement to information is obligatory. One of the statistical methods often used in proteomics is classification: the assignment of subjects to discrete categories, for...article 2007
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Spijkerman, A.M.W. (author), Gall, M.A. (author), Tarnow, L. (author), Twisk, J.W.R. (author), Lauritzen, E. (author), Lund-Andersen, H. (author), Emeis, J. (author), Parving, H.H. (author), Stehouwer, C.D.A. (author), TNO Kwaliteit van Leven (author)Aims: To study whether microalbuminuria, endothelial dysfunction and low-grade inflammation are associated with the presence and progression of diabetic retinopathy. Methods: Patients with Type 2 diabetes (n = 328) attending a diabetes clinic were followed for 10 years and examined annually during the last 7 years. Retinopathy was assessed after...article 2007
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van Doorn, M. (author), Vogels, J. (author), Tas, A. (author), van Hoogdalem, E.J. (author), Burggraaf, J. (author), Cohen, A. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)Aims: To explore the usefulness of metabolomics as a method to obtain a broad array of biomarkers for the pharmacological effects of rosiglitazone (RSG) in plasma and urine samples from patients with type 2 diabetes mellitus (T2DM) and healthy volunteers (HVs). Additionally, we explored the differences in metabolite concentrations between T2DM...article 2007
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Nanni, S. (author), Melandri, G. (author), Hanemaaijer, R. (author), Cervi, V. (author), Tomasi, L. (author), Altimari, A. (author), van Lent, N. (author), Tricoci, P. (author), Bacchi, L. (author), Branzi, A. (author), TNO Kwaliteit van Leven (author)Matrix metalloproteinases (MMPs) are thought to participate in the pathogenesis of coronary artery disease (CAD), particularly in the occurrence of acute coronary syndrome (ACS). Little is known about human in vivo MMP regulation in CAD. The expression and regulation of MMPs and their tissue inhibitors (TIMPs) were evaluated in premature CAD....article 2007
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Meulenbelt, I. (author), Kloppenburg, M. (author), Kroon, H.M. (author), Houwing-Duistermaat, J.J. (author), Garnero, P. (author), Hellio-Le Graverand, M.-P. (author), de Groot, J. (author), Slagboom, P.E. (author), TNO Kwaliteit van Leven (author)Objective: To assess the relationship of biochemical markers and radiographic signs of osteoarthritis (ROA) in the subjects with symptomatic osteoarthritis (OA) at multiple sites of the Genetics osteoARthritis and Progression (GARP) study. Methods: We have measured eight biochemical markers, representing tissue turnover of cartilage, bone,...article 2007
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van Tits, L.J.H. (author), van Heerde, W.L. (author), van der Vleuten, G.M. (author), de Graaf, J. (author), Grobbee, D.E. (author), van de Vijver, L.P.L. (author), Stalenhoef, A.F. (author), Princen, H.M. (author), TNO Kwaliteit van Leven (author)Exogenous radiolabeled annexin A5 is taken up by atherosclerotic tissue. We measured endogenous plasma annexin A5 and circulating oxidized low-density lipoprotein (oxLDL), a biochemical marker of atherosclerosis, in men with either severe angiographically determined coronary stenosis (n = 90) or no or only minor stenosis (n = 96). Men without...article 2007
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Schoonen, W.G.E.J. (author), Kloks, C.P.A.M. (author), Ploemen, J.P.H.T.M. (author), Horbach, G.J. (author), Smit, M.J. (author), Zandberg, P. (author), Mellema, J.R. (author), van Zuylen, C.T. (author), Tas, A.C. (author), van Nesselrooij, J.H.J. (author), Vogels, J.T.W.E. (author), TNO Kwaliteit van Leven (author)1H nuclear magnetic resonance (NMR) spectroscopy of rat urine in combination with pattern recognition analysis was evaluated for early noninvasive detection of toxicity of investigational chemical entities. Bromobenzene (B) and paracetamol (P) were administered at five single oral dosages between 2 and 500 mg/kg and between 6 and 1800 mg/kg,...article 2007
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van der Greef, J. (author), Martin, S. (author), Juhasz, P. (author), Adourian, A. (author), Plasterer, T. (author), Verheij, E.R. (author), McBurney, R.N. (author), TNO Kwaliteit van Leven (author)Systems biology has developed in recent years from a technology-driven enterprise to a new strategic tool in Life Sciences, particularly for innovative drug discovery and drug development. Combining the ultimate in systems phenotyping with in-depth investigations of biomolecular mechanisms will enable a revolution in our understanding of disease...article 2007
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Hunter, D.J. (author), Li, J. (author), LaValley, M. (author), Bauer, D.C. (author), Nevitt, M. (author), de Groot, J. (author), Poole, R. (author), Eyre, D. (author), Guermazi, A. (author), Gale, D. (author), Felson, D.T. (author), TNO Kwaliteit van Leven (author)We used data from a longitudinal observation study to determine whether markers of cartilage turnover could serve as predictors of cartilage loss on magnetic resonance imaging (MRI). We conducted a study of data from the Boston Osteoarthritis of the Knee Study (BOKS), a completed natural history study of knee osteoarthritis (OA). All subjects in...article 2007