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Bos, R. (author), Revet, M. (author), Nieuwenhuizen, W. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)This article describes a two-step bio-immuno assay (BIA), which determines active (free) tissue-type plasminogen activator (t-PA) in (acidified) plasma. Plasma samples, diluted in buffer of pH 6.0, are added to the wells of a microtiter plate containing an immobilised monoclonal antibody which binds t-PA without affecting t-PA activity. After an...article 1994