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Heiniö, R.L. (author), Noort, M.W.J. (author), Katina, K. (author), Alam, S.A. (author), Sozer, N. (author), de Kock, H.L. (author), Hersleth, M. (author), Poutanen, K. (author)Background: Wholegrain foods are known to be health-beneficial but their sensory characteristics may be a limiting factor for consumption. The scientific literature of factors influencing sensory quality of wholegrain foods is rather scarce. Scope and approach: Many cereals like rye, oats, barley and sorghum are actually used mainly as...article 2016
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Zha, Y. (author), Westerhuis, J.A. (author), Muilwijk, B. (author), Overkamp, K.M. (author), Nijmeijer, B.M. (author), Coulier, L. (author), Smilde, A.K. (author), Punt, P.J. (author)Background: Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellulosic biomass. An exometabolomics approach was applied to systematically identify inhibitors in lignocellulosic biomass hydrolysates.Results: We studied the composition and fermentability of 24 different...article 2014
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Jonker, D. (author), Hasselwander, O. (author), Tervilä-Wilo, A. (author), Tenning, P.P. (author), TNO Kwaliteit van Leven (author)Beta-glucans are glucose polymers present in cereal grains, particularly barley and oat. Consumption of these grains or concentrated beta-glucan preparations has been shown to lower blood cholesterol. The present study was conducted to assess the safety of a high purity (>75%) barley beta-glucan (Glucagel™). The product was fed to Wistar rats (5...article 2010
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Maraschin, S.D.F. (author), Caspers, M. (author), Potokina, E. (author), Wülfert, F. (author), Graner, A. (author), Spaink, H.P. (author), Wang, M. (author), TNO Kwaliteit van Leven (author)Different aspects of androgenesis induction have been studied in detail, but little is known about the molecular mechanisms associated with this developmental switch. We have employed macroarrays containing 1421 expressed sequence tags covering the early stages of barley zygotic embryogenesis to compare the gene expression profiles of stress...article 2006
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Time-lapse tracking of barley androgenesis reveals position-determined cell death within pro-embryosMaraschin, S.D.F. (author), Vennik, M. (author), Lamers, G.E.M. (author), Spaink, H.P. (author), Wang, M. (author)Following abiotic stress to induce barley (Hordeum vulgare L.) androgenesis, the development of 794 enlarged microspores in culture was monitored by time-lapse tracking. In total, 11% of the microspores tracked developed into embryo-like structures (type-I pathway), 36% formed multicellular structures (type-II pathway) and 53% of the microspores...article 2005
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TNO Kwaliteit van Leven (author), van den Wijngaard, P.W.J. (author), Sinnige, M.P. (author), Roobeek, I. (author), Reumer, A. (author), Schoonheim, P.J. (author), Mol, J.N.M. (author), Wang, M. (author), de Boer, A.H. (author)Germination of seeds proceeds in general in two phases, an initial imbibition phase and a subsequent growth phase. In grasses like barley, the latter phase is evident as the emergence of the embryonic root (radicle). The hormone abscisic acid (ABA) inhibits germination because it prevents the embryo from entering and completing the growth phase....article 2005
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Maraschin, S.D.F. (author), Gaussand, G. (author), Pulido, A. (author), Olmedilla, A. (author), Lamers, G.E.M. (author), Korthout, H. (author), Spaink, H.P. (author), Wang, M. (author)Androgenesis represents one of the most fascinating examples of cell differentiation in plants. In barley, the conversion of stressed uninucleate microspores into embryo-like structures is highly efficient. One of the bottlenecks in this process is the successful release of embryo-like structures out of the exine wall of microspores. In the...article 2005
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Delaney, B. (author), de Vogel, N. (author), Krul, C.A.M. (author), TNO Voeding (author)Chemicals / CAS: corn oil, 8001-30-7; mitomycin C, 50-07-7, 74349-48-7; Glucans; Mutagensarticle 2004
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Spaenij-Dekking, E.H.A. (author), Kooy-Winkelaar, E.M.C. (author), Nieuwenhuizen, W.F. (author), Drijfhout, J.W. (author), Koning, F. (author), TNO Voeding (author)Background: It is now generally accepted that coeliac disease (CD) is caused by inflammatory T cell responses to gluten peptides bound to HLA-DQ2 or -DQ8 molecules. There is overwhelming evidence that CD patients can mount T cell responses to peptides found in both α-gliadin and γ-gliadin molecules. Assays that would detect the presence or...article 2004
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Delaney, B. (author), Carlson, T. (author), Zheng, G.-H. (author), Hess, R. (author), Knutson, N. (author), Frazer, S. (author), Ostergren, K. (author), van Zijverden, M. (author), Knippels, L. (author), Jonker, D. (author), Penninks, A. (author), TNO Voeding (author)The cholesterol-lowering effect observed following consumption of oats and barley is attributable to the β-glucan component of the soluble fiber fraction of these cereal grains. β-Glucan has also been reported to modulate immune activity, however, few studies have evaluated the hematological effects of β-glucan following oral exposure. In the...article 2003
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de Maraschin, S. (author), Lamers, G.E.M. (author), de Pater, B.S. (author), Spaink, H.P. (author), Wang, M. (author)The members of the 14-3-3 isoform family have been shown to be developmentally regulated during animal embryogenesis, where they take part in cell differentiation processes. 14-3-3 isoform-specific expression patterns were studied in plant embryogenic processes, using barley (Hordeum vulgare L.) microspore embryogenesis as a model system. After...article 2003
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Maraschin, S.D.F. (author), Louwerse, J.D. (author), Lamers, G.E.M. (author), Spaink, H.P. (author), Wang, M. (author)Conserved 14-3-3 proteins have been shown to play regulatory roles in eukaryotic cells, including cell cycle control and differentiation. We were interested in the possible involvement of 14-3-3 proteins in the embryogenic process of barley (Hordeum vulgare L.). Barley microspore-derived embryo development was used as a model system....article 2003
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Delaney, B. (author), Carlson, T. (author), Frazer, S. (author), Zheng, T. (author), Hess, R. (author), Ostergren, K. (author), Kierzek, K. (author), Haworth, J. (author), Knutson, N. (author), Junker, K. (author), Jonker, D. (author), TNO Voeding (author)β-Glucans are water-soluble cell-wall polysaccharides consisting of (1→3,1→4)-linked β-D-glucopyranosyl monomers that comprise a considerable proportion of soluble fiber from certain grains including oats and barley. Consumption of foods containing β-glucan or β-glucan-enriched fractions prepared from these grains lower serum cholesterol...article 2003
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TNO Animal Nutrition (author), Collier, C.T. (author), van der Klis, J.D. (author), Deplancke, B. (author), Anderson, D.B. (author), Gaskins, H.R. (author)Necrotic enteritis (NE) is a worldwide poultry disease caused by the alpha toxin-producing bacterium Clostridium perfringens. Disease risk factors include concurrent coccidial infection and the dietary use of cereal grains high in nonstarch polysaccharides (NSP), such as wheat, barley, rye, and oats. Outbreaks of NE can be prevented or treated...article 2003
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Korthout, H.A.A.J (author), Caspers, M.P.M (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author)Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2...article 2002
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Holtman, W.L. (author), Roberts, M.R. (author), Wang, M. (author)Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms...article 2000
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Holtman, W.L. (author), Roberts, M.R. (author), Oppedijk, B.J. (author), Testerink, C. (author), van Zeijl, M.J. (author), Wang, M. (author)Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were...article 2000
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van Zeijl, M.J. (author), Testerink, C. (author), Kijne, J.W. (author), Wang, M. (author)Expression and post-translational modification of barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, were investigated using isoform-specific antibodies. Although all three isoforms were shown to be present in the cytosolic, the nuclear and the microsomal cell fractions, differences in post-translational modification were identified for the...article 2000
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van Mechelen, J.R. (author), Schuurink, R.C. (author), Smits, M. (author), Graner, A. (author), Douma, A.C. (author), Sedee, N.J.A. (author), Schmitt, N.F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and...article 1999
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van Lonkhuijsen, H.J. (author), Douma, A.C. (author), Angelino, S.A.G.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)New malting barley varieties are annually tested for their malting and brewing potential according to a field trial set-up combined with quality evaluation on pilot scale. To assess the effects of trial year and location on quality evaluation data, a data base consisting of quality data from Dutch malting and brewing trials of new malting barley...article 1998
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