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van der Vossen, J.M.B.M. (author), Kreikamp, A.P. (author), Hatt, V. (author), Ouwens, A.M.T. (author), Brasem, D.J. (author), Heerikhuisen, M. (author), Montijn, R.C. (author)Background: The global COVID-19 pandemic has resulted in a greater interest in improving the ventilation of indoor environments in order to remove aerosolized virus and thus reduce transmission. Air purification systems have been proposed as a solution to improve aerosol removal. Aim: The aim was to determine the efficacy of air purification...article 2023
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Venhorst, J. (author), van der Vossen, J.M.B.M. (author), Agamennone, V. (author)The pathogenic Clostridioides difficile and Clostridium perfringens are responsible for many health care-associated infections as well as systemic and enteric diseases. Therefore, they represent a major health threat to both humans and animals. Concerns regarding increasing antibiotic resistance (related to C. difficile and C. perfringens) have...article 2022
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Selberherr, E. (author), Grau, J.H. (author), Meixner, M. (author), Liere, K. (author), Ercolini, D. (author), de Filippo, C. (author), Caderni, G. (author), Brigidi, P. (author), Turroni, S. (author)The core microbiome, which refers to a set of consistent microbial features across populations, is of major interest in microbiome research and has been addressed by numerous studies. Understanding the core microbiome can help identify elements that lead to dysbiosis, and lead to treatments for microbiome-related health states. However, defining...article 2022
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Bikker, F.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Fidder, A. (author), van der Schans, G.P. (author), TNO Defensie en Veiligheid (author)As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming...article 2007
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Vlaminckx, B.J.M. (author), Schuren, F.H.J. (author), Montijn, R.C. (author), Caspers, M.P.M. (author), Fluit, A.C. (author), Wannet, W.J.B. (author), Schouls, L.M. (author), Verhoef, J. (author), Jansen, W.T.M. (author), TNO Kwaliteit van Leven (author)Group A streptococci (GAS), or Streptococcus pyogenes, are associated with a remarkable variety of diseases, ranging from superficial infections to life-threatening diseases such as toxic-shock-like syndrome (TSS). GAS strains belonging to M types M1 and M3 are associated with TSS. This study aims to obtain insight into the gene profiles...article 2007
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Fekete, A. (author), Vink, A.A. (author), Gaspar, S. (author), Modos, K. (author), Berces, A. (author), Ronto, Gy. (author), Roza, L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Phage T7 can be used as a biological UV dosimeter. Its reading is proportional to the inactivation rate expressed in HT7 units. To understand the influence of phage proteins on the formation of DNA UV photoproducts, cyclobutane pyrimidine dimers (CPD) and (6-4)photoproducts ((6-4)PD) were determined in T7 DNA exposed to UV radiation under...article 1999
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Modos, K. (author), Gáspár, S. (author), Kerekgyarto, T. (author), Vink, A.A. (author), Roza, L. (author), Fekete, A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To estimate the risk of enhanced UV-B radiation due to stratospheric ozone depletion, phage T7 and uracil thin-layer biological dosimeters have been developed, which weight the UV irradiance according to induced DNA damage. To study the molecular basis of the biological effects observed after UV irradiation, the spectral sensitivity curves of...article 1999
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Fekete, A. (author), Vink, A.A. (author), Gaspar, S. (author), Berces, A. (author), Modos, K. (author), Ronto, Gy. (author), Roza, L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The correlation between the biologically effective dose (BED) of a phage T7 biological dosimeter and the induction of cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts ((6-4)PD) in the phage DNA was determined using seven various UV sources. The BED is the inactivation rate of phage T7 expressed in HT7 units. The CPD and (6-4)PD were...article 1998
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Mientjes, E.J. (author), Hochleitner, K. (author), Luiten-Schuite, A. (author), van Delft, J.H.M. (author), Thomale, J. (author), Berends, F. (author), Rajewsky, M.F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)LacZ transgenic mice are suitable for short-term mutagenicity studies in vivo. Mutagenicity in these mice is determined in the lacZ transgene. Since the lacZ gene is of bacterial origin the question has been raised whether DNA-adduct formation and repair in the transgene are comparable to those in total genomic DNA. Mice were treated with N...article 1996
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Mientjes, E.J. (author), Steenwinkel, M.J.S.T. (author), van Delft, J.H.M. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)The recent introduction of the phenyl-β-D-galactopyranoside (P-gal)-based positive-selection system for screening of λlacZ phages originating from the λlacZ transgenic mouse (Muta Mouse) has made the determination of mutant frequencies (MF) a much simpler task. Previously, MF data from these mice have been collected by means of the 5-bromo-4...article 1996
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Leer, R.J. (author), van Luijk, N. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)The DNA sequences of a 2.4 kb plasmid (p353-2) from Lactobacillus pentosus MD353 and a 1.9 kb plasmid (p8014-2) from Lactobacillus plantarum ATCC 8014 show 81.5% overall similarity. Both plasmids carry elements (replication protein gene, plus-origin and minus-origin of replication), which are typical of plasmids that replicate via a rolling...article 1992
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Mattern, I.E. (author), Cocchiarella, L. (author), van Kralingen, C.G. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)Eleven platinum compounds with nitrogen donor ligands, previously tested for anti-tumour activity were studied for induction of prophage lambda and for mutagenicity in the Ames assay, with various strains of Salmonella. The compounds included cis and trans isomers of Pt(II) and Pt(IV) complexes and were tested with and without metabolic...article 1982
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Bleichrodt, J.F. (author), Roos-Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)Under conditions where the reversion of an amber mutant of bacteriophage ?? by ??-rays is enhanced by subjecting the irradiated phage to SOS repair, ??-ray-induced reversion of two T7 ambers is not influenced by this error-prone bacterial repair system. The survival of T7 ??-irradiated under anoxic conditions is somewhat enhanced by SOS repair,...article 1980
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- Bleichrodt, J.F. (author), Roos-Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author) article 1979
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Bleichrodt, J.F. (author), Roos-Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)As in the induction of r mutants in bacteriophage T4 by ??-rays, the radiation-induced reversion of T4 amber mutants to wild-type was found to depend on the product of the DNA-repair gene x of the phage. Neither the efficiency of induction of r mutants nor the efficiency of reversion of ambers was enhanced by the presence of oxygen during...article 1979
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de Mik, G. (author), de Groot, I. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)The mechanisms of inactivation of aerosolized bacteriophage phiX174 in atmospheres containing ozone, cyclohexene, or ozonized cyclohexene were studied by using 32P-labelled phage. The inactivation of the aerosolized phage in clean air or in air containing cyclohexene is due to damage of the protein coat since the deoxyribonucleic acid (DNA)...article 1977
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Bleichrodt, J.F. (author), Roos, A.L.M. (author), Roos Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)Amber mutants of bacteriophage T7 are reverted by γ-rays to pseudo wild-type particles, i.e. particles able to propagate in a suppressorless host. The yield of revertants is much higher when the phage is irradiated in the presence of oxygen than when irradiated anoxically. Under particular gas conditions the efficiency of mutation induction...article 1977
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de Mik, G. (author), de Groot, I. (author), Gerbrandy, J.L.F. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)The effects of ozone and ozonized olefins on aerosol survival of bacteriophage phiX174 were studied. The ozone concentrations used were between 0 and 110 parts/109, giving decay rates up to 0.03 min-1. The olefins used were trans 2 butene and cyclohexene in concentrations of 500 parts/109 and 2.4 parts/106, respectively. Olefins alone have no...article 1977
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van Hemmen, J.J. (author), Meuling, W.J.A. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)The radiosensitization by oxygen of biologically active bacteriophage DNA in bacterial extracts was studied. The oxygen effect in such a system appeared not to be due or only to a minor extent to the presence of endogenous sulphydryl compounds. The components in a cell extract which enable oxygen and other sensitizers to sensitize DNA could not...article 1974
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van Hemmen, J.J. (author), Meuling, W.J.A. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)Paranitroacetophenone (PNAP) and triacetoneamine N oxyl (TAN), which sensitize anoxic cells to ionizing radiation, do not increase the radiosensitivity of bacteriophage DNA irradiated in the dry state, showing that the 'direct action model' of sensitization does not hold in this experimental set up. PNAP and TAN added within 1.5 msec after...article 1973
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