Searched for: subject%3A%22Bacterial%255C%2BProteins%22
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Syvertsson, S. (author), Wang, B. (author), Staal, J. (author), Gao, Y. (author), Kort, R. (author), Hamoen, L.W. (author)
To cope with sudden changes in their environment, bacteria can use a bet-hedging strategy by dividing the population into cells with different properties. This so-called bimodal or bistable cellular differentiation is generally controlled by positive feedback regulation of transcriptional activators. Due to the continuous increase in cell volume...
article 2021
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Molhoek, E.M. (author), van Dijk, A. (author), Veldhuizen, E.J.A. (author), Haagsman, H.P. (author), Bikker, F.J. (author)
A truncated version of host defense peptide chicken cathelicidin-2, C1-15, possesses potent, broad spectrum antibacterial activity. A variant of this peptide, F2,5,12W, which contains 3 phenylalanine to tryptophan substitutions, possesses improved antibacterial activity and lipopolysaccharide (LPS) neutralizing activity compared to C1-15. In...
article 2011
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Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...
article 2009
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Wierckx, N. (author), Ruijssenaars, H.J. (author), de Winde, J.H. (author), Schmid, A. (author), Blank, L.M. (author), TNO Kwaliteit van Leven (author)
The physiological effects of genetic and transcriptional changes observed in a phenol producing mutant of the solvent-tolerant Pseudomonas putida S12 were assessed with metabolic flux analysis. The upregulation of a malate/lactate dehydrogenase encoding gene could be connected to a flux increase from malate to oxaloacetate. A mutation in the...
article 2009
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Volkers, R.J.M. (author), Ballerstedt, H. (author), Ruijssenaars, H. (author), de Bont, J.A.M. (author), de Winde, J.H. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
Pseudomonas putida S12 is well known for its remarkable solvent tolerance. Transcriptomics analysis of this bacterium grown in toluene-containing chemostats revealed the differential expression of 253 genes. As expected, the genes encoding one of the major solvent tolerance mechanisms, the solvent efflux pump SrpABC and its regulatory genes...
article 2009
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Koopman, F.W. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 compounds methanol and formaldehyde as auxiliary substrate. The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic...
article 2009
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TNO-Quality of Life, Business Unit Food and Biotechnology Innovations (author), Meijnen, J.P. (author), Winde, J.H.de (author), Ruijssenaars, H.J. (author)
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...
article 2009
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Paauw, A. (author), Caspers, M.P.M. (author), Leverstein-van Hall, M.A. (author), Schuren, F.H.J. (author), Montijn, R.C. (author), Verhoef, J. (author), Fluit, A.C. (author), TNO Kwaliteit van Leven (author)
Bacterial strains differ in their ability to cause hospital outbreaks. Using comparative genomic hybridization, Enterobacter cloacae complex isolates were studied to identify genetic markers specific for Enterobacter cloacae complex outbreak strains. No outbreak-specific genes were found that were common in all investigated outbreak strains....
article 2009
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Lee, J. (author), Tomchick, D.R. (author), Brautigam, C.A. (author), Machius, M. (author), Kort, R. (author), Hellingwerf, K.J. (author), Gardner, K.H. (author), TNO Kwaliteit van Leven (author), KvL (author)
The Bacillus subtilis KinA protein is a histidine protein kinase that controls the commitment of this organism to sporulate in response to nutrient deprivation and several other conditions. Prior studies indicated that the N-terminal Per-ARNT-Sim domain (PAS-A) plays a critical role in the catalytic activity of this enzyme, as demonstrated by...
article 2008
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ter Beek, A. (author), Keijser, B.J.F. (author), Boorsma, A. (author), Zakrzewska, A. (author), Orij, R. (author), Smits, G.J. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)
The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify potential resistance...
article 2008
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Weelink, S.A.B. (author), Tan, N.C.G. (author), ten Broeke, H. (author), van den Kieboom, C. (author), van Doesburg, W. (author), Langenhoff, A.A.M. (author), Gerritse, J. (author), Junca, H. (author), Stams, A.J.M. (author), TNO Bouw en Ondergrond (author)
A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are 0.6 μm wide and 1 to 2 μm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds with...
article 2008
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Boesten, R.J. (author), de Vos, W.M. (author), TNO Kwaliteit van Leven (author)
Scientific evidence that supports a correlation between our intestinal microbiota and health status has caused significant interest in microbe-host interaction studies. It has generated a paradigm shift from analyzing pathogens to that involving commensal and probiotic bacteria. This review summarizes the interaction mechanisms described for...
article 2008
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TNO Kwaliteit van Leven (author), Kralj, S. (author), van Leeuwen, S.S. (author), Valk, V. (author), Eeuwema, W. (author), Kamerling, J.P. (author), Dijkhuizen, L. (author)
The reuteransucrase enzymes of Lactobacillus reuteri strain 121 (GTFA) and L. reuteri strain ATCC 55730 (GTFO) convert sucrose into alpha-d-glucans (labelled reuterans) with mainly alpha-(1→4) glucosidic linkages (50% and 70%, respectively), plus alpha-(1→6) linkages. In the present study, we report a detailed analysis of various hybrid GTFA/O...
article 2008
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Ruijssenaars, H.J. (author), Sperling, E.M.G.M. (author), Wiegerinck, P.H.G. (author), Brands, F.T.L. (author), Wery, J. (author), de Bont, J.A.M. (author), TNO Kwaliteit van Leven (author)
A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the...
article 2007
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Smither, S.J. (author), Hill, J. (author), van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Titball, R.W. (author), TNO Defensie en Veiligheid (author)
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through...
article 2007
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Vlaminckx, B.J.M. (author), Schuren, F.H.J. (author), Montijn, R.C. (author), Caspers, M.P.M. (author), Fluit, A.C. (author), Wannet, W.J.B. (author), Schouls, L.M. (author), Verhoef, J. (author), Jansen, W.T.M. (author), TNO Kwaliteit van Leven (author)
Group A streptococci (GAS), or Streptococcus pyogenes, are associated with a remarkable variety of diseases, ranging from superficial infections to life-threatening diseases such as toxic-shock-like syndrome (TSS). GAS strains belonging to M types M1 and M3 are associated with TSS. This study aims to obtain insight into the gene profiles...
article 2007
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Ballerstedt, H. (author), Volkers, R.J.M. (author), Mars, A.E. (author), Hallsworth, J.E. (author), Santos, V.A.M.D. (author), Puchalka, J. (author), van Duuren, J. (author), Eggink, G. (author), Timmis, K.N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas...
article 2007
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TNO Kwaliteit van Leven (author), van der Horst, M.A. (author), Arents, J.C. (author), Kort, R. (author), Hellingwerf, K.J. (author)
The bacterial photoreceptor protein photoactive yellow protein (PYP) covalently binds the chromophore 4-hydroxy coumaric acid, tuning (spectral) characteristics of this cofactor. Here, we study this binding and tuning using a combination of pointmutations and chromophore analogs. In all photosensor proteins studied to date the covalent linkage...
article 2007
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Volkers, R.J.M. (author), de Jong, A.L. (author), Hulst, A.G. (author), van Baar, B.L.M. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven TNO Defensie en Veiligheid (author)
The aim of this study was to assess the cellular response of the solvent-tolerant Pseudomonas putida S12 to toluene as the single effector. Proteomic analysis (two-dimensional difference-in-gel-electrophoresis) was used to assess the response of P. putida S12 cultured in chemostats. This approach ensures constant growth conditions, both in the...
article 2006
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van Hijum, S.A.F.T. (author), Kralj, S. (author), Ozimek, L.K. (author), Dijkhuizen, L. (author), van Geel-Schutten, G.H. (author), TNO Kwaliteit van Leven (author)
Lactic acid bacteria (LAB) employ sucrase-type enzymes to convert sucrose into homopolysaccharides consisting of either glucosyl units (glucans) or fructosyl units (fructans). The enzymes involved are labeled glucansucrases (GS) and fructansucrases (FS), respectively. The available molecular, biochemical, and structural information on sucrase...
article 2006
Searched for: subject%3A%22Bacterial%255C%2BProteins%22
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