Banned antibacterial growth promoters in animal feed: Collaborative trial on the liquid chromatography-tandem mass spectrometry method developed in the feedstuffs-radius project

A chemical confirmation method for the identification and quantification of five banned antibacterial growth promoters (AGPs) in animal feed was developed and in-house validated as part of the European Feedstuffs-RADIUS project [1]. To complete the validation process a collaborative trial was performed by eleven laboratories within the European...

Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic

A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel...