Searched for: subject%3A%22Bacillus%22
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Syvertsson, S. (author), Wang, B. (author), Staal, J. (author), Gao, Y. (author), Kort, R. (author), Hamoen, L.W. (author)
To cope with sudden changes in their environment, bacteria can use a bet-hedging strategy by dividing the population into cells with different properties. This so-called bimodal or bistable cellular differentiation is generally controlled by positive feedback regulation of transcriptional activators. Due to the continuous increase in cell volume...
article 2021
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Botschuijver, S. (author), Welting, O. (author), Levin, E. (author), Maria-Ferreira, D. (author), Koch, E. (author), Montijn, R.C. (author), Seppen, J. (author), Hakvoort, T.B.M. (author), Schuren, F.H.J. (author), de Jonge, W.J. (author), van den Wijngaard, R.M. (author)
Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder associated with altered gastrointestinal microflora and increased nociception to colonic distension. This visceral hypersensitivity can be reversed in our rat maternal separation model by fungicides. Menthacarin® is a proprietary combination of essential oils from...
article 2018
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de Oliveira, J.E. (author), van der Hoeven-Hangoor, E. (author), van de Linde, I.B. (author), Montijn, R.C. (author), van der Vossen, J.M.B.M. (author)
The feasibility of establishing probiotic bacteria in the intestine of broiler chickens by in ovo inoculation was investigated, followed by verifying possible subsequent protection against Salmonella Enteriditis infection. In a first study, 7 commercially available probiotics were screened for compatibility with in ovo inoculation. Two of these...
article 2014
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Sijbrandij, T. (author), Kaman, W.E. (author), Ligtenberg, A.J.M. (author), Nazmi, K. (author), Veerman, E.C.I. (author), Bikker, F.J. (author)
Toxin-antitoxin modules are necessary for the mode of action of several antibiotics. One of the most studied toxin-antitoxin modules is the quorum sensing - dependent MazEF system in Escherichia coli. The quorum sensing factor in this system is called the extracellular death factor (EDF), a linear pentapeptide with the sequence NNWNN. In spite...
article 2014
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van Melis, C.C.J. (author), Almeida, C.B. (author), Kort, R. (author), Groot, M.N.N. (author), Abee, T. (author)
In this study, the impact of a range of organic acids and structurally similar alcohols with three to six carbon backbones and increasing lipophilic character, were tested on the germination behavior of B. cereus ATCC 14579 spores. This approach allowed substantiating whether the effectivity of the various compounds was largely dictated by...
article 2012
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Hatanaka, M. (author), Nakamura, Y. (author), Maathuis, A.J.H. (author), Venema, K. (author), Murota, I. (author), Yamamoto, N. (author)
Survival and germination rate of Bacillus subtilis C-3102 spores were investigated in a stomach and small intestine model (TIM-1), while the impact of C-3102 cells that had passed through TIM-1 on human colon microbiota was evaluated in a model of the large intestine (TIM-2). The survival of C-3102 spores in TIM-1 was 99%; 8% of the spores had...
article 2012
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Paauw, A. (author)
MALDI-TOF-MS emerged as a new diagnostic tool in established clinical laboratories. Advantages compared to conventional techniques are that it is a fast, cost-effective, accurate method, which is suitable for high-throughput identification of bacteria by less skilled laboratory personnel because preliminary identification steps are unnecessary....
conference paper 2012
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Brul, S. (author), van Beilen, J. (author), Caspers, M. (author), O'Brien, A. (author), de Koster, C. (author), Oomes, S. (author), Smelt, J. (author), Kort, R. (author), ter Beek, A. (author)
Bacterial spore formers are prime organisms of concern in the food industry. Spores from the genus Bacillus are extremely stress resistant, most notably exemplified by high thermotolerance. This sometimes allows surviving spores to germinate and grow out to vegetative cells causing food spoilage and possible intoxication. Similar issues though...
article 2011
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Abhyankar, W. (author), Beek, A.T. (author), Dekker, H. (author), Kort, R. (author), Brul, S. (author), de Koster, C.G. (author)
Species from the genus Bacillus have the ability to form endospores, dormant cellular forms that are able to survive heat and acid preservation techniques commonly used in the food industry. Resistance characteristics of spores towards various environmental stresses are in part attributed to their coat proteins. Previously, 70 proteins have been...
article 2011
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Caspers, M.P.M. (author), Schuren, F.H.J. (author), van Zuijlen, A.C.M. (author), Brul, S. (author), Montijn, R.C. (author), Abee, T. (author), Kort, R. (author)
Failure of food preservation is frequently caused by thermostable spores of members of the Bacillaceae family, which show a wide spectrum of resistance to cleaning and preservation treatments. We constructed and validated a mixed-species genotyping array for 6 Bacillus species, including Bacillus subtilis, Bacillus licheniformis, Bacillus...
article 2011
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Koopman, F.W. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 compounds methanol and formaldehyde as auxiliary substrate. The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic...
article 2009
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Lee, J. (author), Tomchick, D.R. (author), Brautigam, C.A. (author), Machius, M. (author), Kort, R. (author), Hellingwerf, K.J. (author), Gardner, K.H. (author), TNO Kwaliteit van Leven (author), KvL (author)
The Bacillus subtilis KinA protein is a histidine protein kinase that controls the commitment of this organism to sporulate in response to nutrient deprivation and several other conditions. Prior studies indicated that the N-terminal Per-ARNT-Sim domain (PAS-A) plays a critical role in the catalytic activity of this enzyme, as demonstrated by...
article 2008
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ter Beek, A. (author), Keijser, B.J.F. (author), Boorsma, A. (author), Zakrzewska, A. (author), Orij, R. (author), Smits, G.J. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)
The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify potential resistance...
article 2008
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Smelt, J.P.P.M. (author), Bos, A.P. (author), Kort, R. (author), Brul, S. (author), TNO Kwaliteit van Leven (author), KvL (author)
Spores of Bacillus subtilis were subjected to relatively mild heat treatments in distilled water and properties of these spores were studied. These spores had lost all or part of their dipicolinic acid (DPA) depending on the severity of the heat treatment. Even after relatively mild heat treatments these spore lost already a small but...
article 2008
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TNO Kwaliteit van Leven (author), Kort, R. (author), Keijser, B.J. (author), Caspers, M.P.M. (author), Schuren, F.H. (author), Montijn, R. (author)
Background: In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias...
article 2008
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Ruijssenaars, H.J. (author), Sperling, E.M.G.M. (author), Wiegerinck, P.H.G. (author), Brands, F.T.L. (author), Wery, J. (author), de Bont, J.A.M. (author), TNO Kwaliteit van Leven (author)
A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the...
article 2007
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Werf, M.J.v.d. (author), Overkamp, K.M. (author), Muilwijk, B. (author), Coulier, L. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)
Achieving metabolome data with satisfactory coverage is a formidable challenge in metabolomics because metabolites are a chemically highly diverse group of compounds. Here we present a strategy for the development of an advanced analytical platform that allows the comprehensive analysis of microbial metabolomes. Our approach started with in...
article 2007
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Keijser, B.J.F. (author), ter Beek, A. (author), Rauwerda, H. (author), Schuren, F. (author), Montijn, R. (author), van der Spek, H. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)
Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we...
article 2007
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Oomes, S.J.C.M. (author), van Zuijlen, A.C.M. (author), Hehenkamp, J.O. (author), Witsenboer, H. (author), van der Vossen, J.M.B.M. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)
Spore-forming bacteria can be a problem in the food industry, especially in the canning industry. Spores present in ingredients or present in the processing environment severely challenge the preservation process since their thermal resistance may be very high. We therefore asked the question which bacterial spore formers are found in a typical...
article 2007
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TNO Defensie en Veiligheid (author), van Wuijckhuijse, A.L. (author), Degenhardt, C.E.A.M. (author), de Groote, F.P.J. (author), van Baar, B.L.M. (author), Kientz, C.E. (author)
conference paper 2007
Searched for: subject%3A%22Bacillus%22
(1 - 20 of 38)

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