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Berghuis, N.F. (author), Mars-Groenendijk, R.H. (author), Busker, R.W. (author), Paauw, A. (author), van Leeuwen, H.C. (author)CRISPR–Cas (CC)-based detection technologies have some exceptional features, which hold the promise of developing into the nextgeneration diagnostic platforms. One of these features is the ability to trigger non-specific single-stranded DNA/RNA cleavage activity after specific target recognition and Cas enzyme activation. This cleavage activity...article 2022
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Velders, A. (author), Ossendrijver, M. (author), Keijser, B.J.F. (author), Saggiomo, V. (author)Development of a novel LAMP device which is cheap, reusable, and can be produced in large amounts in a short period of time. The device was designed such not to require chemical exothermic reactions, have limited waste produced and with a minimum cost of the device as a whole. The device was tested for the detection of SARS-CoV2 RNA. Funding:...article 2021
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Smits, M. (author), Meijerink, M. (author), Le, T.M. (author), Knulst, A. (author), de Jong, A. (author), Caspers, M.P.M. (author), Lima, E.S. (author), Babe, L. (author), Ladics, G. (author), McClain, S. (author), Houben, G.F. (author), Verhoeckx, K. (author)Background: Food proteins differ in their allergenic potential. Currently, there is no predictive and validated bio-assay to evaluate the allergenicity of novel food proteins. The objective of this study was to investigate the potential of a human peripheral blood mononuclear cell (PBMC) gene expression assay to identify biomarkers to predict...article 2021
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Byrne, F.P. (author), Nussbaumer, C.M. (author), Savin, E.J. (author), Milescu, R.A. (author), McElroy, C.R. (author), Clark, J.H. (author), van Vugt-Lussenburg, B.M.A. (author), van der Burg, B. (author), Meima, M.Y. (author), Buist, H.E. (author), Kroese, E.D. (author), Hunt, A.J. (author), Farmer, T.J. (author)Three dipolar aprotic solvents were designed to possess high dipolarity and low toxicity: N,N,N′,N′-tetrabutylsuccindiamide (TBSA), N,N′-diethyl-N,N′-dibutylsuccindiamide (EBSA), and N,N′-dimethyl-N,N′-dibutylsuccindiamide (MBSA). They were synthesized catalytically by using a K60 silica catalyst in a solventless system. Their water...article 2020
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Meijerink, M. (author), van den Broek, T.J. (author), Dulos, R. (author), Garthoff, J. (author), Knippels, L. (author), Knipping, K. (author), Harthoorn, L. (author), Houben, G. (author), Verschuren, L. (author), van Bilsen, J. (author)To assess the safety and efficacy of oral immune interventions, it is important and required by regulation to assess the impact of those interventions not only on the immune system, but also on other organs such as the gut as the porte d'entrée. Despite clear indications that the immune system interacts with several physiological functions of...article 2019
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Reisinger, K. (author), Blatz, V. (author), Brinkmann, J. (author), Downs, T.R. (author), Fischer, A. (author), Henkler, F. (author), Hoffmann, S. (author), Krul, C. (author), Liebsch, M. (author), Luch, A. (author), Pirow, R. (author), Reus, A.A. (author), Schulz, M. (author), Pfuhler, S. (author)Recently revised OECD Testing Guidelines highlight the importance of considering the first site-of-contact when investigating the genotoxic hazard. Thus far, only in vivo approaches are available to address the dermal route of exposure. The 3D Skin Comet and Reconstructed Skin Micronucleus (RSMN) assays intend to close this gap in the in vitro...article 2018
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Botschuijver, S. (author), Welting, O. (author), Levin, E. (author), Maria-Ferreira, D. (author), Koch, E. (author), Montijn, R.C. (author), Seppen, J. (author), Hakvoort, T.B.M. (author), Schuren, F.H.J. (author), de Jonge, W.J. (author), van den Wijngaard, R.M. (author)Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder associated with altered gastrointestinal microflora and increased nociception to colonic distension. This visceral hypersensitivity can be reversed in our rat maternal separation model by fungicides. Menthacarin® is a proprietary combination of essential oils from...article 2018
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Leist, M. (author), Ghallab, A. (author), Graepel, R. (author), Marchan, R. (author), Hassan, R. (author), Bennekou, S.H. (author), Limonciel, A. (author), Vinken, M. (author), Schildknecht, S. (author), Waldmann, T. (author), Danen, E. (author), van Ravenzwaay, B. (author), Kamp, H. (author), Gardner, I. (author), Godoy, P. (author), Bois, F.Y. (author), Braeuning, A. (author), Reif, R. (author), Oesch, F. (author), Drasdo, D. (author), Höhme, S. (author), Schwarz, M. (author), Hartung, T. (author), Braunbeck, T. (author), Beltman, J. (author), Vrieling, H. (author), Sanz, F. (author), Forsby, A. (author), Gadaleta, D. (author), Fisher, C. (author), Kelm, J. (author), Fluri, D. (author), Ecker, G. (author), Zdrazil, B. (author), Terron, A. (author), Jennings, P. (author), van der Burg, B. (author), Dooley, S. (author), Meijer, A.H. (author), Willighagen, E. (author), Martens, M. (author), Evelo, C. (author), Mombelli, E. (author), Taboureau, O. (author), Mantovani, A. (author), Hardy, B. (author), Koch, B. (author), Escher, S. (author), van Thriel, C. (author), Cadenas, C. (author), Kroese, D. (author), van de Water, B. (author), Hengstler, J.G. (author)Adverse outcome pathways (AOPs) are a recent toxicological construct that connects, in a formalized, transparent and quality-controlled way, mechanistic information to apical endpoints for regulatory purposes. AOP links a molecular initiating event (MIE) to the adverse outcome (AO) via key events (KE), in a way specified by key event...article 2017
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Raaphorst, R.M. (author), Savolainen, H. (author), Cantore, M. (author), van de Steeg, E. (author), van Waarde, A. (author), Colabufo, N.A. (author), Elsinga, P.H. (author), Lammertsma, A.A. (author), Windhorst, A.D. (author), Luurtsema, G. (author)Positron emission tomography (PET) imaging of P-glycoprotein (P-gp) in the blood-brain barrier can be important in neurological diseases where P-gp is affected, such as Alzheimer’s disease. Radiotracers used in the imaging studies are present at very small, nanomolar, concentration, whereas in vitro assays where these tracers are characterized,...article 2017
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Luijten, M. (author), Olthof, E.D. (author), Hakkert, B.C. (author), Rorije, E. (author), van der Laan, J.-W. (author), Woutersen, R.A. (author), van Benthem, J. (author)Assessment of genotoxic and carcinogenic potential is considered one of the basic requirements when evaluating possible human health risks associated with exposure to chemicals. Test strategies currently in place focus primarily on identifying genotoxic potential due to the strong association between the accumulation of genetic damage and cancer...article 2016
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van der Burg, B. (author), Pieterse, B. (author), Rorije, E. (author), Lewin, G. (author), van der Linden, S.C. (author), Man, H.Y. (author), Piersma, A.H. (author), Mangelsdorf, I. (author), Wolterbeek, A.P.M. (author), Kroese, E.D. (author), van Vugt-Lussenburg, B.M.A. (author)There is a great need for alternative testing methods for reproductive toxicants that are practical, fast, cost-effective and easy to interpret. Previously we followed a pragmatic approach using readily available tests, which was successful in predicting reproductive toxicity of chemicals [13]. This initial battery still contained apical tests...article 2015
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Dusinska, M. (author), Boland, S. (author), Saunders, M. (author), Juillerat-Jeanneret, L. (author), Tran, L. (author), Pojana, G. (author), Marcomini, A. (author), Volkovova, K. (author), Tulinska, J. (author), Knudsen, L.E. (author), Gombau, L. (author), Whelan, M. (author), Collins, A.R. (author), Marano, F. (author), Housiadas, C. (author), Bilanicova, D. (author), Halamoda Kenzaoui, B. (author), Correia Carreira, S. (author), Magdolenova, Z. (author), Fjellsbo, L.M. (author), Huk, A. (author), Handy, R. (author), Walker, L. (author), Barancokova, M. (author), Bartonova, A. (author), Burello, E. (author), Castell, J. (author), Cowie, H. (author), Drlickova, M. (author), Guadagnini, R. (author), Harris, G. (author), Harju, M. (author), Heimstad, E.S. (author), Hurbankova, M. (author), Kazimirova, A. (author), Kovacikova, Z. (author), Kuricova, M. (author), Liskova, A. (author), Milcamps, A. (author), Neubauerova, E. (author), Palosaari, T. (author), Papazafiri, P. (author), Pilou, M. (author), Poulsen, M.S. (author), Ross, B. (author), Runden-Pran, E. (author), Sebekova, K. (author), Staruchova, M. (author), Vallotto, D. (author), Worth, A. (author)In spite of recent advances in describing the health outcomes of exposure to nanoparticles (NPs), it still remains unclear how exactly NPs interact with their cellular targets. Size, surface, mass, geometry, and composition may all play a beneficial role as well as causing toxicity. Concerns of scientists, politicians and the public about...article 2015
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Mesens, N. (author), Crawfordb, A.D. (author), Menke, A. (author), Hung, P.D. (author), van Goethem, F. (author), Nuyts, R. (author), Hansen, E. (author), Wolterbeek, A. (author), van Gompel, J. (author), de Witte, P. (author), Esguerra, C.V. (author)Drug-induced liver injury (DILI) is poorly predicted by single-cell-based assays, probably because of the lack of physiological interactions with other cells within the liver. An intact whole liver system such as one present in zebrafish larvae could provide added value in a screening strategy for DILI; however, the possible occurrence of other...article 2015
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Kroese, E.D. (author), Bosgra, S. (author), Buist, H.E. (author), Lewin, G. (author), van der Linden, S.C. (author), Man, H.Y. (author), Piersma, A.H. (author), Rorije, E. (author), Schulpen, S.H.W. (author), Schwarz, M. (author), Uibel, F. (author), van Vugt-Lussenburg, B.M.A. (author), Wolterbeek, A.P.M. (author), van der Burg, B. (author)Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery...article 2015
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Buist, H.E. (author), Devito, S. (author), Goldbohm, R.A. (author), Stierum, R.H. (author), Venhorst, J. (author), Kroese, E.D. (author)Carbon capture and storage (CCS) technologies are considered vital and economic elements for achieving global CO2 reduction targets, and is currently introduced worldwide (for more information on CCS, consult for example the websites of the International Energy Agency (http://www.iea.org/topics/ccs/) and the Global CCS Institute (http://www...article 2015
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Teunis, M.A.T. (author), Spiekstra, S.W. (author), Smits, M. (author), Adriaens, E. (author), Eltze, T. (author), Galbiati, V. (author), Krul, C.A.M. (author), Landsiedel, R. (author), Pieters, R. (author), Reinders, J. (author), Roggen, E. (author), Corsini, E. (author), Gibbs, S. (author)This study describes the international ring trial of the epidermal-equivalent (EE) sensitizer potency assay. This assay does not distinguish a sensitizer from a non-sensitizer, but may classify known skin sensitizers according to their potency. It assesses the chemical concentration resulting in 50% cytotoxicity (EE-EC50) or the 2-fold increase...article 2014
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Zimmer, B. (author), Pallocca, G. (author), Dreser, N. (author), Foerster, S. (author), Waldmann, T. (author), Westerhout, J. (author), Julien, S. (author), Krause, K.H. (author), van Thriel, C. (author), Hengstler, J.G. (author), Sachinidis, A. (author), Bosgra, S. (author), Leist, M. (author)Developmental toxicity in vitro assays have hitherto been established as stand-alone systems, based on a limited number of toxicants. Within the embryonic stem cell-based novel alternative tests project, we developed a test battery framework that allows inclusion of any developmental toxicity assay and that explores the responses of such test...article 2014
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Piersma, A.H. (author), Bosgra, S. (author), van Duursen, M.B.M. (author), Hermsen, S.A.B. (author), Jonker, L.R.A. (author), Kroese, E.D. (author), van der Linden, S.C. (author), Man, H. (author), Roelofs, M.J.E. (author), Schulpen, S.H.W. (author), Schwarz, M. (author), Uibel, F. (author), van Vugt-Lussenburg, B.M.A. (author), Westerhout, J. (author), Wolterbeek, A.P.M. (author), van der Burg, B. (author)The application of alternative methods in developmental and reproductive toxicology is challenging in view of the complexity of mechanisms involved. A battery of complementary test systems may provide a better prediction of developmental and reproductive toxicity than single assays. We tested twelve compounds with varying mechanisms of toxic...article 2013
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Aranda, A. (author), Sequedo, L. (author), Tolosa, L. (author), Quintas, G. (author), Burello, E. (author), Castell, J.V. (author), Gombau, L. (author)No consensus exists on how to address possible toxicity of nanomaterials as they interfere with most in vitro screening tests based on colorimetric and fluorimetric probes such as the dichloro-dihydrofluorescein diacetate (DCFH-DA) assay for detection of oxidative species. In the present research, nanomaterial interaction with DCFH-DA was...article 2013
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Jacobs, J.H. (author), Fuertes, E. (author), Krop, E.J.M. (author), Spithoven, J. (author), Tromp, P. (author), Heederik, D.J.J. (author)Objectives: To describe associations among swimming, respiratory health, allergen sensitisation and Clara cell protein 16 (CC16) levels in Dutch schoolchildren. Trichloramine levels in swimming pool air were determined to assess potential exposure levels. Methods: Respiratory health and pool attendance information was collected from 2359...article 2012