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Gouka, R.J. (author), Hessing, J.G.M. (author), Punt, P.J. (author), Stam, H. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an...article 1996
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Gouka, R.J. (author), Hessing, J.G.M. (author), Stam, H. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awarnori pyrG mutant strain constructed by a selection strategy for gene-replacement in fungi. Second, a vector with a homologous pyrG selection marker containing a defined mutation at a site...article 1995
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van Gemeren, I.A. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was...article 1995
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van den Brink, J.M. (author), van Zeijl, C.M.J. (author), Brons, J.F. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In this paper, we describe the cloning and molecular characterization of the Aspergillus niger cytochrome P450 reductase (CPR) gene, cprA. Attempts to clone the cprA gene by heterologous hybridization techniques were unsuccessful. Using the polymerase chain reaction (PCR) with degenerate primers based on conserved regions found in cpr genes from...article 1995
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Punt, P.J. (author), Veldhuisen, G. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Although the application of filamentous fungi, such as Aspergillus niger for the production of extracellular proteins is well established for several decades, hardly any information is available about the molecular mechanisms of the process of protein secretion in these organisms. Two lines of research initiated towards a systematic analysis of...article 1994
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Verdoes, J.C. (author), Punt, P.J. (author), Stouthamer, A.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO Centraal Instituut voor Voedingsonderzoek TNO (author)The regulation of transcription of the glucoamylase-encoding gene (glaA) of Aspergillus niger was studied. To facilitate this study a reporter strain containing a fusion of the glaA promoter (P(glaA)) of A. niger to the β- glucuronidase-encoding gene (uidA) of Escherichia coli was constructed. To analyze whether regulatory proteins are involved...article 1994
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Verdoes, J.C. (author), Calil, M.R. (author), Punt, P.J. (author), Debets, A.J.M. (author), Swart, K. (author), Stouthamer, A.H. (author), van den Hondel, C.A.M.J.J. (author)A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF gel electrophoresis and Southern ana lysis. All of the eight linkage groups (LGs), with the exception of LG VII, have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger. Using a LG VII...article 1994
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Smits, J.P. (author), Knol, W. (author), Bol, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Two fungal strains, Aspergillus clavatus II-9 and Fusarium oxysporum @ 149, proved to be capable of degrading sinigrin and sinalbin. During the degradation of sinigrin by whole cells of the Aspergillus strain, allylcyanide accumulated in the liquid incubation mixture. After a maximum concentration had been reached, the concentration of...article 1993
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Medisch Biologisch Laboratorium TNO (author), Verdoes, J.C. (author), Punt, P.J. (author), Schrickx, J.M. (author), van Verseveld, H.W. (author), Stouthamer, A.H. (author), van den Hondel, C.A.M.J.J. (author)A strategy, based on the usage of the amdS selection marker and a cosmid vector containing four copies of the glucoamylase gene (glaA), was developed to obtain glucoamylase (GLA)-overproducing A. niger strains. With this strategy, fungal strains carrying up to 200 copies of the glaA gene could be isolated at a relatively high frequency. In each...article 1993
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Punt, P.J. (author), Kramer, C. (author), Kuyvenhoven, A. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene...article 1992
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Mattern, I.E. (author), van Noort, J.M. (author), van den Berg, P. (author), Archer, D.B. (author), Roberts, I.N. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)In the present study, the extracellular protease activity in a strain of the filamentous fungus Aspergillus niger was investigated and mutant strains deficient in the production of extracellular proteases were isolated. The major protease, which is responsible for 80-85% of the total activity, is aspergillopepsin A, a protein of ca. 43 kDa, the...article 1992
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- Medisch Biologisch Laboratorium TNO (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), van Gorcom, R.F.M. (author) article 1992
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van Noort, J.M. (author), van den Berg, P. (author), Mattern, I.E. (author), Medisch Biologisch Laboratorium TNO (author)A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel...article 1991
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Punt, P.J. (author), Zegers, N.D. (author), Busscher, M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of Aspergillus nidulans. Both the homologous gpdA and the heterologous Escherichia coli β-galactosidase ...article 1991
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Medisch Biologisch Laboratorium TNO (author), Punt, P.J. (author), Dingemanse, M.A. (author), Kuyvenhoven, A. (author), Soede, R.D.M. (author), Pouvels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Analysis of the promoter region of the highly expressed Aspergillus nidulans gpdA gene is described. The nucleotide (nt) sequence of a 1.3-kb region upstream from the ATG was determined. Comparison with promoter regions of other Aspergillus and Neurospora genes revealed several regions similar sequence. Both random and site-specific mutations...article 1990
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van Gorcom, R.F.M. (author), Boschloo, J.G. (author), Kuijvenhoven, A. (author), Lange, J. (author), van Vark, A.J. (author), Bos, C.J. (author), van Balken, J.A.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)The gene coding for benzoate-para-hydroxylase (bphA) of Aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. The nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcription start and termination sites were...article 1990
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Medisch Biologisch Laboratorium TNO (author), Kos, T. (author), Kuijvenhoven, A. (author), Hessing, H.G.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Molecular Sequence Numbers: GENBANK: X07071; Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; DNA Restriction Enzymes, EC 3.1.21; Tryptophan, 73-22-3article 1988
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Davidse, L.C. (author), Flach, W. (author), Centrum voor Plantenfysiologisch Onderzoek Nationale Raad voor Landbouwkundig Onderzoek TNO (author)Chemicals/CAS: benomyl, 17804-35-2; carbon 14, 14762-75-5; colchicine, 64-86-8; fuberidazole, 3878-19-1; griseofulvin, 126-07-8; mebendazole, 31431-39-7; melatonin, 73-31-4; nocodazole, 31430-18-9; parbendazole, 14255-87-9; podophyllotoxin, 518-28-5; tiabendazole, 148-79-8; vinblastine, 865-21-4; Benzimidazoles; Carbamates; Colchicine, 64-86-8;...article 1977
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Davidse, L.C. (author), Werkgroep voor Inwendige Therapie van Plantenziekten TNO (author)Methyl benzimidazol 2 yl carbamate was metabolized by Aspergillus nidulans mycelium to two metabolites, one of which was identified as methyl 5 hydroxybenzimidazol 2 yl carbamate. This compound was further converted to a second metabolite which was not identified. Conversion rate was highest when the culture medium was depleted of nutrients....article 1976
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Hoppe, H.H. (author), Kerkenaar, A. (author), Kaars Sypesteyn, A. (author), Organisch Chemisch Instituut TNO (author)3 Phenylindole is an antimicrobial compound active towards many fungi and gram positive bacteria. At 5 μg/ml it inhibits growth of Aspergillus niger. Higher concentrations (50 μg/ml) also suppress spore germination; they do not kill the fungus. Dry weight of the fungus still increases for 1 or 2 days after fungicide treatment. The toxicant has...article 1976