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Snopov, S.A. (author), de Gruijl, F.R. (author), Roza, L. (author), van der Leun, J.C. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Studies of UV-induced skin cancers show that malignisation of skin cells, as well as alterations in anti-tumor immune control, are triggered by UV-induced lesions in cellular DNA. Such lesions can probably appear in the human mononuclear leukocytes (lymphocytes) during exposure of skin to sunlight. With the aim of studying the processing of UV...article 2004
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Koppelman, S.J. (author), Wensing, M. (author), Ertmann, M. (author), Knulst, A.C. (author), Knol, E.F. (author), TNO Voeding (author)Background: A number of allergenic proteins in peanut has been described and the relative importance of these allergens is yet to be determined. Objectives: We have investigated the relevance of previously identified peanut allergens in well-characterized peanut-allergic patients by in vitro, ex vivo and in vivo assays. Methods: Thirty-two adult...article 2004
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Nagelkerken, L. (author), Haspels, I. (author), van Rijs, W. (author), Blauw, B. (author), Ferrant, J.L. (author), Hess, D.M. (author), Garber, E.A. (author), Taylor, F.R. (author), Burkly, L.C. (author), Gaubius Instituut TNO (author)It has been demonstrated that anti-CD154 mAb treatment effectively inhibits the development of experimental autoimmune encephalomyelitis (EAE). However, although it appears to prevent the induction of Th1 cells and reactivation of encephalitogenic T cells within the CNS, little information is available regarding the involvement of alternative...article 2004
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Mittag, D. (author), Akkerdaas, J. (author), Ballmer-Weber, B.K. (author), Vogel, L. (author), Wensing, M. (author), Becker, W.M. (author), Koppelman, S.J. (author), Knulst, A.C. (author), Helbling, A. (author), Hefle, S.L. (author), van Ree, R. (author), Vieths, S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)We recently described patients with soybean allergy mainly mediated by cross-reactivity to birch pollen allergens. A majority of those patients were reported to have peanut allergy. We sought to study the occurrence of peanut allergy in patients allergic to birch pollen and characterized the Bet v 1-homologous peanut allergen Ara h 8....article 2004
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van Wijk, F. (author), Hartgring, S. (author), Koppelman, S.J. (author), Pieters, R. (author), Knippels, L.M.J. (author), TNO Voeding (author)Background: Peanut allergy is known for its severity and persistence through life. Several peanut proteins have been identified as allergenic and are indicated as Ara h 1-7. Very little is known about the mechanisms that underlie sensitization to peanut proteins. Objective: The purpose of the present study was to reveal the immune responses that...article 2004
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Spaenij-Dekking, E.H.A. (author), Kooy-Winkelaar, E.M.C. (author), Nieuwenhuizen, W.F. (author), Drijfhout, J.W. (author), Koning, F. (author), TNO Voeding (author)Background: It is now generally accepted that coeliac disease (CD) is caused by inflammatory T cell responses to gluten peptides bound to HLA-DQ2 or -DQ8 molecules. There is overwhelming evidence that CD patients can mount T cell responses to peptides found in both α-gliadin and γ-gliadin molecules. Assays that would detect the presence or...article 2004
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Levasseur, A. (author), Pagès, S. (author), Fierobe, H.P. (author), Navarro, D. (author), Punt, P. (author), Belaïch, J.P. (author), Asther, M. (author), Record, E. (author)A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergilhis niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA (FAEA-Doc), whereas no chimeric protein was produced when the bacterial dockerin domain was...article 2004
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Albers, R. (author), van der Wielen, R.P.J. (author), Brink, E.J. (author), Hendriks, H.F.J. (author), Dorovska-Taran, V.N. (author), Mohede, I.C.M. (author), TNO Voeding (author)Objectives: To study the effects of two different mixtures of the main conjugated linoleic acid (CLA) isomers cis-9, trans-11 CLA and trans-10, cis-12 CLA on human immune function. Design: Double-blind, randomized, parallel, reference-controlled intervention study. Subjects and intervention: Seventy-one healthy males aged 31-69y received one of...article 2003
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van Hensbergen, Y. (author), Broxterman, H.J. (author), Peters, E. (author), Rana, S. (author), Elderkamp, Y.W. (author), van Hinsbergh, V.W.M. (author), Koolwijk, P. (author), Gaubius Instituut TNO (author)The aminopeptidase inhibitor bestatin has been shown to have anti-angiogenic effects in a number of model systems. These effects are thought to result from inhibition of CD13 activity. Because tumor angiogenesis can evolve in a fibrin-rich stroma matrix we have studied for the first time the effects of bestatin on microvascular endothelial...article 2003
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Joosten, V. (author), Lokman, C. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Voeding (author)In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of...article 2003
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de Maraschin, S. (author), Lamers, G.E.M. (author), de Pater, B.S. (author), Spaink, H.P. (author), Wang, M. (author)The members of the 14-3-3 isoform family have been shown to be developmentally regulated during animal embryogenesis, where they take part in cell differentiation processes. 14-3-3 isoform-specific expression patterns were studied in plant embryogenic processes, using barley (Hordeum vulgare L.) microspore embryogenesis as a model system. After...article 2003
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Vogels, R. (author), Zuijdgeest, D. (author), van Rijnsoever, R. (author), Hartkoorn, E. (author), Damen, I. (author), de Béthune, M.-P. (author), Kostense, S. (author), Penders, G. (author), Helmus, N. (author), Koudstaal, W. (author), Cecchini, M. (author), Wetterwald, A. (author), Sprangers, M. (author), Lemckert, A. (author), Ophorst, O. (author), Koel, B. (author), van Meerendonk, M. (author), Quax, P. (author), Panitti, L. (author), Grimbergen, J. (author), Bout, A. (author), Goudsmit, J. (author), Havenga, M. (author)Replication-deficient human adenovirus type 5 (Ad5) can be produced to high titers in complementing cell lines, such as PER.C6, and is widely used as a vaccine and gene therapy vector. However, preexisting immunity against Ad5 hampers consistency of gene transfer, immunological responses, and vector-mediated toxicities. We report the...article 2003
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Nieuwenhuizen, W.F. (author), Pieters, R.H.H. (author), Knippels, L.M.J. (author), Jansen, M.C.J.F. (author), Koppelman, S.J. (author)Coeliac disease is a T-cell-mediated autoimmune disease of the small intestine that is induced by ingestion of gluten proteins from wheat, barley, or rye. We postulate that Candida albicans is a trigger in the onset of coeliac disease. The virulence factor of C albicans - hyphal wall protein 1 (HWP1) - contains aminoacid sequences that are...article 2003
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van der Gaag, B. (author), Spath, S. (author), Dietrich, H. (author), Stigter, E. (author), Boonzaaijer, G. (author), van Osenbruggen, T. (author), Koopal, K. (author)An immunochemical biosensor assay for the detection of multiple mycotoxins in a sample is described.The inhibition assay is designed to measure four different mycotoxins in a single measurement, following extraction, sample clean-up and incubation with an appropriate cocktail of anti-mycotoxin antibodies.The different mycotoxins could be...article 2003
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Bellou, A. (author), Saint-Laudy, J. (author), Knippels, L. (author), Montémont, C. (author), Vauthier, E. (author), Gerard, P. (author), Pellegrom, H. (author), Koerkamp, E.K. (author), Lesesve, J.F. (author), Guéant, J.L. (author), Lambert, H. (author), Mallié, J.P. (author), TNO Voeding (author)Anaphylactic shock is an immunoglobulin E (IgE)-dependent hypersensitivity. Biological tests like leucocyte histamine release (LHR) and human basophil activation (HBA), frequently used in human allergy, reflect both the amount of IgE fixed on cells and the cellular reactivity. To assess whether serum-specific IgE from Brown Norway (BN) rats...article 2003
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Knippels, L.M.J. (author), Penninks, A.H. (author), TNO Voeding (author)The need for widely accepted and validated animal models to test the potential allergenicity and potency of novel (biotechnology-derived) proteins has become an important issue for their safety evaluation.In this article, we summarize the results of the development of an oral sensitization protocol for food proteins in the rat. Young Brown...article 2003
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Vančíková, Z. (author), Lodinová-Žádníková, R. (author), Radl, J. (author), Tlaskalová-Hogenová, H. (author)The development of levels of secretory immunoglobulins (SIgs) in newborns' saliva was examined under physiological conditions and after artificial colonization with nonpathogenic, probiotic bacterial strain E. coli O83. Higher levels of secretory immunoglobulin M (SIgM) and secretory immunoglobulin A (SIgA) were detected in the saliva of breast...article 2003
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Heikkilä, P. (author), Teronen, O. (author), Hirn, M.Y. (author), Sorsa, T. (author), Tervahartiala, T. (author), Salo, T. (author), Konttinen, Y.T. (author), Halttunen, T. (author), Moilanen, M. (author), Hanemaaijer, R. (author), Laitinen, M. (author)Background. Bisphosphonates reduce the bone metastasis formation and angiogenesis but the exact molecular mechanisms involved are unclear. Progelatinase A (proMMP-2; 78 KDa) is activated up during the tumor spread and metastasis by a cell surface-associated matrix metalloproteinase (membrane-type matrix metalloproteinase [MT1-MMP] or MMP-14)....article 2003
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O'Connor, D.H. (author), Mothe, B.R. (author), Weinfurter, J.T. (author), Fuenger, S. (author), Rehrauer, W.M. (author), Jing, P. (author), Rudersdorf, R.R. (author), Liebl, M.E. (author), Krebs, K. (author), Vasquez, J. (author), Dodds, E. (author), Loffredo, J. (author), Martin, S. (author), McDermott, A.B. (author), Allen, T.M. (author), Wang, C. (author), Doxiadis, G.G. (author), Montefiori, D.C. (author), Hughes, A. (author), Burton, D.R. (author), Allison, D.B. (author), Wolinsky, S.M. (author), Bontrop, R. (author), Picker, L.J. (author), Watkins, D.I. (author)Certain major histocompatibility complex class I (MHC-I) alleles are associated with delayed disease progression in individuals infected with human immunodeficiency virus (HIV) and in macaques infected with simian immunodeficiency virus (SIV). However, little is known about the influence of these MHC alleles on acute-phase cellular immune...article 2003
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TNO Voeding (author), Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G.A. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author)The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI...article 2003