- document
-
Haaijman, J.J. (author), Brinkhof, J. (author), Instituut voor Experimentele Gerontologie TNO (author), IVEG (author)The IgG fractions of rabbit antisera specific for the Fc part of mouse IgA, IgM, and the four subclasses of IgG (1, 2a, 2b, and 3) were coupled covalently to Sepharose beads by the cyanogen bromide method. The beads were then incubated with different dilutions of mouse serum. The mouse immunoglobulins (Ig) binding to the beads were demonstrated...article 1977
- document
-
Lubbe, F.H. (author), Rossi, G. (author), Zaalberg, O.B. (author), Medisch Biologisch Laboratorium TNO (author)A method is described for isolation of antibody forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37°C. During incubation rabbit anti mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were...article 1976
- document
-
Instituut voor experimentele gerontologie TNO (author), Bloemmen, F.J. (author), Radl, J. (author), Haaijman, J.J. (author)Highly purified MOPC 21 IgG1, MOPC 173 IgG(2a), MOPC 195 IgG(2b), MOPC 104 E IgM, and MOPC 315 IgA paraproteins, heterogeneous mouse IgG, Fab and Fc fragments of heterogeneous IgG were prepared and coupled to Sepharose beads. These beads were then used as artificial substrates to test the specificity of fluorescent antisera against mouse...article 1976
- document
-
Instituut voor experimentele gerontologie TNO (author), Haaijman, J.J. (author), van Dalen, J.P.R. (author)In order to obtain microfluorometric standards for fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC), Sephadex G 25 Superfine beads (diameter 10-40 μ) were aminated with aminoethylsulphuric acid and stained with FITC or TRITC. Several parameters of the reaction were assessed. A high degree of linearity was...article 1974