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Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author), Baan, R.A. (author), Lansbergen, M.J. (author), de Bruin, P.A.F. (author), Willems, M.I. (author), Lohman, P.H.M. (author)Exposure of cells to chemical carcinogens and mutagens may result in the formation of DNA adducts, which can give rise to mutations in the genome and to cellular transformation. Methods to measure DNA-adduct formation may be useful for 'biomonitoring', to establish exposure of laboratory animals or humans to DNA-damaging agents. For such...article 1985
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Lubbe, F.H. (author), Hooijkaas, H. (author), Preesman, A.A. (author), Medisch Biologisch Laboratorium TNO (author)The effect of X-irradiation upon murine antibody-forming cell precursors (B cells) was established in cultures of spleen cells stimulated with the B cell mitogen lipopolysaccharide (LPS). At day 5 and 7 the numbers of IgM- and IgG2-secreting cells were determined in cultures of irradiated and nonirradiated spleen cells. From these numbers a D(o)...article 1982
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Lubbe, F.H. (author), Brocades Zaalberg, O. (author), Medisch Biologisch Laboratorium TNO (author)Helper T cells specific for sheep erythrocytes were generated in lethally irradiated mice. The helper effect exerted by these cells was determined in vitro in spleen cell cultures. Irradiation of the helper cells in vivo or in vitro with 1-9 Gy resulted in a bimodal relation between radiation dose and helper effect when measured either...article 1982
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van Pelt Verkuil, E. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity....article 1981
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Stitz, L. (author), Kreeftenberg, J.G. (author), Loggen, H.G. (author), Balner, H. (author), Primatencentrum TNO (author)62 Rhesus monkeys were tested at different times after vaccinia virus infection for virus-specific induction of lymphocyte proliferation in vitro or antibody production in vivo. No association was found between identifiable RhLA-controlled antigens and the strength of the cellular proliferative and/or humoral response. Chemicals/CAS: Antibodies,...article 1981
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Schellekens, H. (author), Weimar, W. (author), de Reus, A. (author), Bolhuis, R.L.H. (author), Cantell, K. (author), Instituut voor Experimentele Gerontologie TNO (author)Chemicals/CAS: Adjuvants, Immunologic; Antibodies, Viral; Interferons, 9008-11-1article 1981
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Creemers, P. (author), Radiobiologisch Instituut TNO (author)Sera from normal mice and mice bearing murine mammary tumor virus (MTV)-induced tumors showed cytotoxic reactions to the MTV-producing Mm5mt/cl cell line. The reaction could be blocked by the addition of MTV, but not of purified gp52 and p28. In sera from tumor bearers, cytotoxic responses ranged from 15 to 66%; reactivity was generally highest...article 1981
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Gaubius Instituut TNO (author), Havekes, L. (author), Vermeer, B.J. (author), Brugman, T. (author), Emeis, J. (author)Chemicals/CAS: Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDLarticle 1981
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Schuit, H.R.E. (author), Hijmans, W. (author), Instituut voor experimentele gerontologie TNO (author)The presence of surface-associated immunoglobulins and Fc receptors on mononuclear cells from normal human blood waas investigated by the direct immunofluorescence technique combined with phase-contrast microscopy. Formaldehyde-fixed cells were compared to unfixed cells and to cells preincubated at 37??C. In the unfixed samples a separate...article 1980
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- Radiobiologisch Instituut TNO (author), Mulder, J.H. (author) article 1980
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Westenbrink, F. (author), Koornstra, W. (author), Creemers, P. (author), Radiobiologisch Instituut TNO (author)Antisera raised in rabbits against purified components gp52, p28 and p12 of murine mammary tumor virus (MuMTV) were first characterized by means of immunodiffusion, immunoelectrophoresis and radioimmunoassay. No significant crossreactivity could be detected. The 3 polypeptides were detected by means of these antisera in the cytoplasm of murine...article 1979
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Radl, J. (author), de Glopper, E. (author), Schuit, H.R.E. (author), Zurcher, C. (author), Instituut voor experimentele gerontologie TNO (author)Transplantation experiments in the C57BL/KaLwRij mouse model of idiopathic paraproteinemia (IP) showed that an IP-producing clone can be further propagated in young, lethally irradiated mice and also equally as well in nonirradiated recipients by a bone marrow and/or spleen cell transfer. The latency period before the original paraprotein was...article 1979
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Bartlema, H.C. (author), Braunius, R. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: Adjuvants, Immunologic; Pertussis Vaccine; Tetanus Toxoidarticle 1979
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Widmer, M.B. (author), Balner, H. (author), Primatencentrum TNO (author)Ten antisera containing antibodies directed against specificities controlled by the Ia1 locus of rhesus monkeys were tested for their ability to inhibit the MLC reaction. In cultures with responding cells from the serum producer, all sera with anti-Ia1, activity depressed the stimulatory capacity of cells carrying the Ia1 antigen in question. In...article 1978
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Creemers, P. (author), Ouwehand, J. (author), Bentveizen, P. (author), Radiobiologisch Instituut TNO (author)The vaccines used in this study were derived from purified murine mammary tumor virus (MuMTV) preparations. Approximately 60% of the protein fractions consisted of the major viral membrane glycoprotein gp52. Inoculation sc of 10 pg MuMTV-S-derived vaccine significantly delayed the appearance of primary mammary tumors in GR and BALB/cfC3H mice ...article 1978
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Haaijman, J.J. (author), Brinkhof, J. (author), Instituut voor Experimentele Gerontologie TNO (author), IVEG (author)The IgG fractions of rabbit antisera specific for the Fc part of mouse IgA, IgM, and the four subclasses of IgG (1, 2a, 2b, and 3) were coupled covalently to Sepharose beads by the cyanogen bromide method. The beads were then incubated with different dilutions of mouse serum. The mouse immunoglobulins (Ig) binding to the beads were demonstrated...article 1977
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Lubbe, F.H. (author), Rossi, G. (author), Zaalberg, O.B. (author), Medisch Biologisch Laboratorium TNO (author)A method is described for isolation of antibody forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37°C. During incubation rabbit anti mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were...article 1976
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Instituut voor experimentele gerontologie TNO (author), Bloemmen, F.J. (author), Radl, J. (author), Haaijman, J.J. (author)Highly purified MOPC 21 IgG1, MOPC 173 IgG(2a), MOPC 195 IgG(2b), MOPC 104 E IgM, and MOPC 315 IgA paraproteins, heterogeneous mouse IgG, Fab and Fc fragments of heterogeneous IgG were prepared and coupled to Sepharose beads. These beads were then used as artificial substrates to test the specificity of fluorescent antisera against mouse...article 1976
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Instituut voor experimentele gerontologie TNO (author), Haaijman, J.J. (author), van Dalen, J.P.R. (author)In order to obtain microfluorometric standards for fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC), Sephadex G 25 Superfine beads (diameter 10-40 μ) were aminated with aminoethylsulphuric acid and stained with FITC or TRITC. Several parameters of the reaction were assessed. A high degree of linearity was...article 1974