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Mehrtens, A. (author), Licha, T. (author), Broers, H.P. (author), Burke, V. (author)The purpose of this long-term experiment was on gaining more insights into the environmental behaviour of veterinary antibiotics in the subsurface after application with manure. Therefore, manure spiked with a bromide tracer and eight antibiotics (enrofloxacin, lincomycin, sulfadiazine, sulfamethazine, tetracycline, tiamulin, tilmicosin and...article 2020
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Kolkman, D.G.E. (author), Rijnders, M.E.B. (author), Wouters, M.G.A.J. (author), Dommelen, P.V. (author), de Groot, C.J.M. (author), Fleuren, M.A.H. (author)Problem: Despite the introduction of preventive guidelines, no decrease in the incidence of early onset infection was observed. Background: Early onset group B streptococcal (EOGBS) infection is an important cause of neonatal morbidity and mortality. Aim: Our study was conducted to determine adherence to three guideline-based group B...article 2020
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Keijser, B.J.F. (author), Agamennone, V. (author), van den Broek, T.J. (author), Caspers, M. (author), van de Braak, A. (author), Bomers, R. (author), Havekes, M. (author), Schoen, E. (author), van Baak, M. (author), Mioch, D. (author), Bomers, L. (author), Montijn, R.C. (author)Background: Antibiotic therapy is commonly used in animal agriculture. Antibiotics excreted by the animals can contaminate farming environments, resulting in long term exposure of animals to sub-inhibitory levels of antibiotics. Little is known on the effect of this exposure on antibiotic resistance. In this study, we aimed to investigate the...article 2019
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Agamennone, V. (author), Krul, C.A.M. (author), Rijkers, G. (author), Kort, R. (author)BACKGROUND Antibiotic-associated diarrhea (AAD) is a side-effect frequently associated with the use of broad spectrum antibiotics. Although a number of clinical studies show that co-administration of specific probiotics reduces the risk for AAD, there is still unclarity among healthcare professionals on the recommendation of probiotic products....article 2019
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Agamennone, V. (author), van Straalen, J. (author), Brouwer, A. (author), de Boer, T.E. (author), Hensbergen, P.J. (author), Zaagman, N. (author), Braster, M. (author), van Straalen, N.M. (author), Roelofs, D. (author), Janssens, T.K.S. (author)Antibiotic resistance necessitates the search for new bioactive compounds with novel mechanisms of action. Natural products derived from bacteria and fungi are widely used in the field of medicine and new environments can be explored as sources of antimicrobials. Bacteria associated with springtails have shown high inhibitory activity against...article 2019
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Agamennone, V. (author), Krul, C.A.M. (author), Rijkers, G. (author), Kort, R. (author)Background Antibiotic-associated diarrhea (AAD) is a side-effect frequently associated with the use of broad spectrum antibiotics. Although a number of clinical studies show that co-administration of specific probiotics reduces the risk for AAD, there is still unclarity among healthcare professionals on the recommendation of probiotic products....article 2018
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Botschuijver, S. (author), Welting, O. (author), Levin, E. (author), Maria-Ferreira, D. (author), Koch, E. (author), Montijn, R.C. (author), Seppen, J. (author), Hakvoort, T.B.M. (author), Schuren, F.H.J. (author), de Jonge, W.J. (author), van den Wijngaard, R.M. (author)Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder associated with altered gastrointestinal microflora and increased nociception to colonic distension. This visceral hypersensitivity can be reversed in our rat maternal separation model by fungicides. Menthacarin® is a proprietary combination of essential oils from...article 2018
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Rashid, M.U. (author), Zaura, E. (author), Buijs, M.J. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Nord, C.E. (author), Weintraub, A. (author)The purpose of the study was to assess the effect of ciprofloxacin (500 mg twice daily for 10 days) or clindamycin (150 mg 4 times daily for 10 days) on the fecal microbiota of healthy humans for a period of 1 year as compared to placebo. Two different methods, culture and microbiome analysis, were used. Fecal samples were collected for analyses...article 2015
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Ladirat, S.E. (author), Schols, H.A. (author), Nauta, A. (author), Schoterman, M.H.C. (author), Schuren, F.H.J. (author), Gruppen, H. (author)In order to elaborate on the impact of amoxicillin treatment on the in vitro fermentation of specific structures of galacto-oligosaccharides (GOS), GOS was fractionated based on its degree of polymerization (DP) and the fermentation of individual DPs was studied. Different DP fractions of GOS and different isomeric structure within a DP fraction...article 2014
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Ladirat, S.E. (author), Schoterman, M.H.C. (author), Rahaoui, H. (author), Mars, M. (author), Schuren, F.H.J. (author), Gruppen, H. (author), Nauta, A. (author), Schols, H.A. (author)In the present double-blind, randomised, parallel intervention study, the effects of the intake of galacto-oligosaccharides (GOS) on the gut microbiota of twelve healthy adult subjects (aged 18-45 years with a normal BMI (18-25 kg/m2)) receiving amoxicillin (AMX) treatment were determined. All the subjects were treated with AMX (375 mg; three...article 2014
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High-throughput analysis of the impact of antibiotics on the human intestinal microbiota compositionLadirat, S.E. (author), Schols, H.A. (author), Nauta, A. (author), Schoterman, M.H.C. (author), Keijser, B.J.F. (author), Montijn, R.C. (author), Gruppen, H. (author), Schuren, F.H.J. (author)Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inoculum from healthy adults was...article 2013
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Kapoerchan, V.V. (author), Knijnenburg, A.D. (author), Keizer, P. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Otero, J.M. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)A series of gramicidin S derivatives 4-15 are presented that have four ornithine residues as polar protonated side chains and two central hydrophobic amino acids with unaltered turn regions. These peptides were screened against human erthrocytes and our standard panel of Gram negative- and Gram positive bacteria, including four MRSA strains....article 2012
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Mariman, R. (author), Kremer, S.H.A. (author), van Erk, M. (author), Lagerweij, T. (author), Koning, F. (author), Nagelkerken, L. (author)Background: Host-microbiota interactions in the intestinal mucosa play a major role in intestinal immune homeostasis and control the threshold of local inflammation. The aim of this study was to evaluate the efficacy of probiotics in the recurrent trinitrobenzene sulfonic acid (TNBS)-induced colitis model and gain more insight into protective...article 2012
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Knijnenburg, A.D. (author), Kapoerchan, V.V. (author), Grotenbreg, G.M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Otero, J.M. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), Ravensbergen, B. (author), Nibbering, P.H. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)In this paper, we describe the crystal structure of previously reported ring-extended gramicidin S (GS) derivative 2 (GS14K4), containing a d-amino acid residue in one of the β-strand regions. This structure is in agreement with a previously reported modeling study of the same molecule. The polar side chain of the additional d-amino acid residue...article 2011
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van Dijk, A. (author), Molhoek, E.M. (author), Bikker, F.J. (author), Yu, P.L. (author), Veldhuizen, E.J.A. (author), Haagsman, H.P. (author)The broad-spectrum defense system based on host defense peptides (HDPs) is evolutionary very old and many invertebrates rely on this system for protection from bacterial infections. However, in vertebrates the system remained important in spite of the superposition of a very sophisticated adaptive immune system. The cathelicidins comprise a...article 2011
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Kapoerchan, V.V. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), de Otero, J.M. (author), Ferraces-Casais, P. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van Doorn, J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author)The cyclic decapeptide gramicidin S (GS) was used as a model for the evaluation of four turn mimetics. For this purpose, one of the D-Phe-Pro two-residue turn motifs in the rigid cyclic β-hairp0in structure of GS was replaced with morpholine amino acids (MAA 2-5), differing in stereochemistry and length of the side-chain. The conformational...article 2010
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Kapoerchan, V.V. (author), Knijnenburg, A.D. (author), Niamat, M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Nibbering, P.H. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Otero, J.M. (author), Llamas-Saiz, A.L. (author), van Raaij, M.J. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)The cyclic cationic antimicrobial peptide gramicidin S (GS) is an effective topical antibacterial agent that is toxic for human red blood cells (hemolysis). Herein, we present a series of amphiphilic derivatives of GS with either two or four positive charges and characteristics ranging between very polar and very hydrophobic. Screening of this...article 2010
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Tuin, A.W. (author), Palachanis, D.K. (author), Buizert, A. (author), Grotenbreg, G.M. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)The synthesis of three new analogues of the cyclic cationic antimicrobial peptide Gramicidin S is described. These derivatives contain a modified turn region in which the DPhe-Pro motif has been replaced by a constrained furanoid sugar amino acid or a flexible linear aminoethoxy acetic acid moiety. Structural analysis revealed conformational...article 2009
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Welling, M.M. (author), Ferro-Flores, G. (author), Pirmettis, I. (author), Brouwer, C.P.J.M. (author)Infection specific radiopharmaceuticals can be used for diagnosis, decision-making in therapy and treatment follow-up. Research has been ongoing to develop infection specific markers since clinically used tracers cannot discriminate between infection and inflammation. A specific radiopharmaceutical for infection imaging should satisfy the...article 2009
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Knijnenburg, A.D. (author), Spalburg, E. (author), de Neeling, A.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Grotenbreg, G.M. (author), van der Marel, G.A. (author), Overkleeft, H.S. (author), Overhand, M. (author), TNO Defensie en Veiligheid (author)(Chemical Equation Presented) A series of ring-extended gramicidin S (GS) derivatives containing furanoid sugar amino acids were evaluated. Although the extended GS derivatives have a less well-defined secondary structure as determined by NMR and CD, some derivatives show an improved biological profile, namely, an increased antibacterial...article 2009