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Reimann, J. (author), Burger, H. (author), Radiobiologisch Instituut TNO (author)
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. Medium,...
article 1979
document
Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)
Highly purified suspensions of parenchymal, endothelial and Kupffer cells were prepared from the rat liver. The respective roles of these cell classes in the degradation of proteins was investigated by analysing the cellular distribution of two lysomal proteases. The specific arginine naphthylamidase activity was 2 times higher in Kupffer cells...
article 1977
document
Instituut voor experimentele gerontologie TNO (author), Knook, D.L. (author), Blansjaar, N. (author), Sleyster, E.C. (author)
Non-parenchymal cell suspensions were prepared from rat livers by three different methods based on a collagenase, a pronase and a combined collagenase-pronase treatment. The highest yield of Kupffer and endothelial cells was obtained with the pronase treatment. Attempts were made for a further purification of these cells by Metrizamide density...
article 1977