Searched for: subject:"Animals"
(81 - 100 of 183)

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Kypreos, K.E. (author), van Dijk, K.W. (author), van der Zee, A. (author), Havekes, L.M. (author), Zannis, V.I. (author), Gaubius Instituut TNO (author)
Apolipoprotein (apo) E has been implicated in cholesterol and triglyceride homeostasis in humans. At physiological concentration apoE promotes efficient clearance of apoE-containing lipoprotein remnants. However, high apoE plasma levels correlate with high plasma triglyceride levels. We have used adenovirus-mediated gene transfer in apoE...
article 2001
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Woutersen, R.A. (author), Jonker, D. (author), Stevenson, H. (author), te Biesebeek, J.D. (author), Slob, W. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
The OECD study design, aimed at obtaining a no-observed-adverse-effect level (NOAEL), may be suboptimal for deriving a benchmark dose. Therefore the present subacute (28-day) study was carried out to evaluate a multiple dose study design and to compare the results with the common OECD design. Seven groups of 10 female rats each were...
article 2001
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Maas, W.J.M. (author), Leeman, W.R. (author), Groten, J.P. (author), van de Sandt, J.J.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Precision-cut liver slices are frequently used to study hepatic toxicity and metabolism of xenobiotics in vitro. Successful cryopreservation techniques will enhance an efficient and economic use of scarcely available (human) liver tissue. For primary hepatocytes, slow freezing has been accepted as the best approach towards successful...
article 2000
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de Beer, F. (author), van Dijk, K.W. (author), Jong, M.C. (author), van Vark, L.C. (author), van der Zee, A. (author), Hofker, M.H. (author), Fallaux, F.J. (author), Hoeben, R.C. (author), Smelt, A.H.M. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)
The apolipoprotein E2 (Lys146→Gln) variant is associated with a dominant form of familial dysbetalipoproteinemia. Heterozygous carriers of this variant have elevated levels of plasma triglycerides, cholesterol, and apolipoprotein E (apoE). It was hypothesized that the high amounts of triglycerides in the very low density lipoprotein (VLDL)...
article 2000
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Post, S.M. (author), de Roos, B. (author), Vermeulen, M. (author), Afman, L. (author), Jong, M.C. (author), Dahlmans, V.E.H. (author), Havekes, L.M. (author), Stellaard, F. (author), Katan, M.B. (author), Princen, H.M.G. (author), Gaubius Instituut TNO (author)
Cafestol, a diterpene present in unfiltered coffee, potently increases serum cholesterol levels in humans. So far, no suitable animal model has been found to study the biochemical background of this effect. We determined the effect of cafestol on serum cholesterol and triglycerides in different mouse strains and subsequently studied its...
article 2000
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Lake, B.G. (author), Rumsby, P.C. (author), Price, R.J. (author), Cunninghame, M.E. (author), TNO BIBRA TNO Kwaliteit van Leven (author)
The objective of this study was to evaluate species differences in the hepatic effects of three potent rodent peroxisome proliferators, namely methylclofenapate (MCP), ciprofibrate (CIP) and Wy-14,643 (WY), particularly with respect to effects on replicative DNA synthesis and transforming growth factor-β1 (TGF-β1) gene expression. Male Sprague...
article 2000
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van Eck, M. (author), Herijgers, N. (author), van Dijk, K.W. (author), Havekes, L.M. (author), Hofker, M.H. (author), Groot, P.H.E. (author), van Berkel, T.J.C. (author), Gaubius Instituut TNO (author)
The effect of monocyte/macrophage-derived wild-type mouse apolipoprotein E (apoE), human apoE3-Leiden, and human apoE2 on serum cholesterol levels and the development of atherosclerosis in apoE-deficient (apoe-/-) mice was investigated by using bone marrow transplantation (BMT). At 4 weeks after BMT, murine apoe+/+ bone marrow reduced serum...
article 2000
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Maas, W.J.M. (author), de Graaf, I.A.M. (author), Schoen, E.D. (author), Koster, H.J. (author), van de Sandt, J.J.M. (author), Groten, J.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A number of studies on the cryopreservation of precision-cut liver slices using various techniques have been reported. However, the identification of important factors that determine cell viability following cryopreservation is difficult because of large differences between the various methods published. The aim of this study was to evaluate...
article 2000
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Izeboud, C.A. (author), Monshouwer, M. (author), Witkamp, R.F. (author), van Miert, A.S.J. (author), TNO Voeding (author)
During infection and inflammation drug disposition and hepatic metabolism are markedly affected in mammals. Pro-inflammatory mediators play an important role in the suppression of (cytochrome-P450-mediated) drug metabolism. Inflammatory mediators like cytokines, nitric oxide (NO), reactive oxygen species (ROS) and eicosanoids are released by...
article 2000
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Edwards, A.J. (author), Price, R.J. (author), Renwick, A.B. (author), Lake, B.G. (author), TNO BIBRA TNO Kwaliteit van Leven (author)
The ability of coumarin to induce UDS in male Sprague-Dawley CD rat hepatocytes in vivo was assessed using the unscheduled DNA synthesis (UDS) assay. From a preliminary toxicity study the oral maximum tolerated dose (MTD) of coumarin was determined to be 320mg/kg body weight. For the UDS studies, rats were treated with 0 (corn oil control), 32 ...
article 2000
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Graaf, I.A.M. (author), Tajima, O. (author), Groten, J.P. (author), Wolterbeek, A.P.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2,2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI...
article 2000
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Spotti, M. (author), Maas, R.F.M. (author), de Nijs, C.M. (author), Fink-Gremmels, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The effects of the mycotoxin fumonisin B1 (FB1) on the hepatic cytochrome P450 system were investigated in male rats dosed daily by oral gavage with 3 mg FB1 per kg body weight for 9 consecutive days. FB1 treatment resulted in a reduced weight gain. At the same time, CYP2E activity was increased, which is considered to mark the metabolic changes...
article 2000
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Hempenius, R.A. (author), Lina, B.A.R. (author), Haggitt, R.C. (author)
Arachidonic acid oil (ARA-oil) derived from the fungus Mortierella alpina for use in infant nutrition was tested in a subchronic (13-week) oral toxicity study in rats, preceded by an in utero exposure phase. The ARA-oil was administered as admixture to the rodent diet at dose levels of 3000 ppm, 15,000 ppm and 75,000 ppm. An additional high-dose...
article 2000
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Rensen, P.C.N. (author), Jong, M.C. (author), van Vark, L.C. (author), van der Boom, H. (author), Hendriks, W.L. (author), van Berkel, T.J.C. (author), Biessen, E.A.L. (author), Havekes, L.M. (author), Gaubius instituut TNO (author)
Apolipoprotein E (apoE) is an important determinant for the uptake of triglyceride-rich lipoproteins and emulsions by the liver, but the intracellular pathway of apoE following particle internalization is poorly defined. In the present study, we investigated whether retroendocytosis is a unique feature of apoE as compared with apoB by studying...
article 2000
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TNO Pharma (author), Hoebe, K.H.N. (author), Monshouwer, M. (author), Witkamp, R.F. (author), Fink-Gremmels, J. (author), van Miert, A.S.J.P.A.M. (author)
article 2000
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Lansink, M. (author), Jong, M. (author), Bijsterbosch, M. (author), Bekkers, M. (author), Toet, K. (author), Havekes, L. (author), Emeis, J. (author), Kooistra, T. (author), Gaubius instituut TNO (author)
Several clinical studies have demonstrated an inverse relationship between circulating levels of estrogen and tissue-type plasminogen activator (t-PA). The present study was designed to test the hypothesis that estrogen lower plasma levels of t-PA by increasing its clearance from the bloodstream. 17α-Ethinyl estradiol (EE) treatment resulted in...
article 1999
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Koopen, N.R. (author), Post, S.M. (author), Wolters, H. (author), Havinga, R. (author), Stellaard, F. (author), Boverhof, R. (author), Kuipers, F. (author), Princen, H.M.G. (author), Gaubius instituut TNO (author)
Effects of 17α-ethinylestradiol (EE) on the neutral and acidic biosynthetic pathways of bile salt (BS) synthesis were evaluated in rats with an intact enterohepatic circulation and in rats with long-term bile diversion to induce BS synthesis. For this purpose, bile salt pool composition, synthesis of individual BS in vivo, hepatic activities,...
article 1999
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van Puymbroeck, M. (author), Kuilman, M.E.M. (author), Maas, R.F.M. (author), Witkamp, R.F. (author), Leyssens, L. (author), van Miert, A.S.J.P.A.M. (author), Hendriks, L. (author), van der Zande, D.J.M. (author), Adriaensens, P. (author), Jacobs, M.-P. (author), Raus, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The metabolism of the illegal growth promoter ethylestrenol (EES) was evaluated in bovine liver cells and subcellular fractions of bovine liver preparations. Incubations with bovine microsomal preparations revealed that EES is extensively biotransformed into norethandrolone (NE), another illegal growth promoter. Furthermore, incubations of...
article 1999
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van Dijk, K.W. (author), van Vlijmen, B.J.M. (author), van 't Hof, H.B. (author), van der Zee, A. (author), Santamarina-Fojo, S. (author), van Berkel, T.J.C. (author), Havekes, L.M. (author), Hofker, M.H. (author), TNO Preventie en Gezondheid (author)
To investigate the quantitative requirement for apolipoprotein (apo) E in the clearance of lipoproteins via the non-low density lipoprotein (LDL) receptor mediated pathway, human APOE was overexpressed at various levels in the livers of mice deficient for both the endogenous Apoe and Ldlr genes (Apoe-/- · Ldlr-/-) using adenovirus-mediated gene...
article 1999
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Bogaards, J.J.P. (author), Venekamp, J.C. (author), Salmon, F.G.C. (author), van Bladeren, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In the present study, the enzymatic conjugation of the isoprene monoepoxides 3,4 epoxy-3-methyl-1-butene (EPOX-I) and 3,4-epoxy-2-methyl-1-butene (EPOX-II) with glutathione was investigated, using purified glutathione S-transferases (GSTs) of the α, μ, π and θ-class of rat and man. HPLC analysis of incubations of EPOX-I and EPOX-II with [35S...
article 1999
Searched for: subject:"Animals"
(81 - 100 of 183)

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