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van Hinsbergh, V.W.M. (author), Bertina, R.M. (author), van Wijngaarden, A. (author), van Tilburg, N.H. (author), Emeis, J.J. (author), Haverkate, F. (author), Gaubius instituut TNO (author)
Confluent cultures of endothelial cells from human umbilical cord were used to study the effect of activated human protein C (APC) on the production of plasminogen activators, plasminogen activator-inhibitor, and factor VIII-related antigen. Addition of APC to the cells in a serum-free medium did not affect the production of tissue-type...
article 1985
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Emeis, J.J. (author), Kluft, C. (author), Gaubius instituut TNO (author)
Platelet-activating factor (PAF-acether; 1-O-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF...
article 1985
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van Pelt Verkuil, E. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)
Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity....
article 1981